Zwitterion-Pair chromatography of nucleotides and related species

Knox, John H.; Jurand, Jadwiga

doi:10.1016/s0021-9673(00)80283-6

Cited by 68 publications

(18 citation statements)

References 14 publications

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“…These were as described by Harvey et al (1982), except that some samples for nucleotide analysis were extracted in trichloroacetic acid (6%, w/v, final concentrations) and assayed by the method of Knox & Jurand (1981) by using a reverse-phase highpressure-liquid-chromatographic column after extensive washing with water-saturated diethyl ether.…”

Section: Assaysmentioning

confidence: 99%

The effect of [Ca2+] and [H+] on the functional recovery of rat brain synaptosomes from anoxic insult in vitro

Harvey¹,

Booth²,

Clark³

1983

Biochemical Journal

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The energy status (as measured by the ATP/ADP ratio), oxidative metabolism (14CO2 output) and neurotransmitter synthesis ( [14C]acetylcholine production) by rat brain synaptosomes utilizing [U-14C]glucose has been studied. The ability of anoxia in vitro to permanently alter these parameters was investigated with reference to external [Ca2+] and [H+]. It has previously been shown that anoxic damage to synaptosomal preparations is only apparent when their metabolism is stimulated by veratridine [Harvey, Booth & Clark (1982) Biochem. J. 206, 433-439]. It is concluded that low [Ca2+] ameliorates, and high [H+] exacerbates, the damage sustained by veratridine-stimulated anoxic synaptosomes. The combined effects of low pH, anoxia and veratridine stimulation on synaptosomal metabolism most closely approximated to the irreversible damage to brain metabolism observed during acute hypoxia in vivo [Booth, Harvey & Clark (1983) J. Neurochem. 40, 106-110]. Suitably treated synaptosomal preparations may therefore be usefully employed as models to study impaired neurotransmitter synthesis in vivo.

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Section: Assaysmentioning

confidence: 99%

The effect of [Ca2+] and [H+] on the functional recovery of rat brain synaptosomes from anoxic insult in vitro

Harvey¹,

Booth²,

Clark³

1983

Biochemical Journal

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“…Interestingly, the k' of a strong organic acid, p-toluenesulfonic acid, decreased rapidly with increasing the pH value of mobile phase, while that of zwitterion compounds, phenylalanine, leucine and alanine, had a volcano at pH about 4. (1) p-toluenesulfonic acid, (2) benzylamine, (3) aniline, (4) benzoic acid, (5) 1-rkphthylaectic acid, (6) phenvlalanine, (7) aniline.…”

Section: Resultsmentioning

confidence: 99%

Multifunctional Zwitterion-Exchange Stationary Phase for HPLC

Chou

Yang

1996

Journal of Liquid Chromatography & Related Technologies

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A zwitterion-exchange stationary phase (ZIXSP) was prepared for the separation of the mixture of organic bases, organic acids and amino acids by high performance liquid chromatography (HPLC). It was synthesized by the reaction of cyanuric chloride with sulfanilic acid and then bonded onto the silica gel on which the silane coupling agent was preboiided. The chromatographic behaviours of the prepared ZIXSP revealed that the capacity factors of each analyte varied with the pH value of the mobile phase. By applying the concept of acid-base equilibrium the correlation W e e n the capacity factor on the ZIXSP and pH of the mobile phase could be explained reasonably. Based on our results, it is concluded that ZIXSP is a zwitterion exchanger that additionally provides R-X chargetransfer interaction and hydrophobic interaction functions.

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“…In 1981, Knox and Jurand [3] separated nucleotides, which have both positive and negative charges, on a reversed-phase column using an eluent containing a zwitterion, (1.25 mM 11-aminodecanoic acid) in addition to 75 mM ammonium phosphate. The retention mechanism was attributed to the formation of a quadripole between the zwitterion and two oppositely charged sites on the nucleotide.…”

Section: Simultaneous Separation Of Anions and Cationsmentioning

confidence: 99%