Zygospore initiation, development and germination in Phycomyces blakesleeanus

Hocking, D.

doi:10.1016/s0007-1536(67)80031-7

Cited by 18 publications

(10 citation statements)

References 16 publications

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“…Asn given as a single N-source liberates Am by the action of asparaginase, which is more active in cultures grown in the dark than in the light (Hilgenberg et al, 1987). As the progress of mating continues well in the dark (Hocking, 1967;, in the case of SlY, the accumulation of Am may be sufficient to inhibit mating. This implies that the nitrogen metabolic pathway is essentially involved in the mating responses of this fungus, especially in zygospore development.…”

Section: Discussionmentioning

confidence: 99%

“…The mating process has been divided into seven steps (A-G), with a somewhat ambiguous classification (Hocking, 1967). Sutter (1975) clearly classified the process into eight developmental stages ($1-8): formation of zygophores as swollen and highly-branched hyphae (Sl); intertwining of two zygophores of different mating types ($2); progametangium formation upright in the air after enlargement of the paired zygophores ($3); looping of progametangial cells due to splitting in the middle region of the enlarged tongs ($4); formation of gametangia and suspensors along both progametangia by delimitation with furrowing septa ($5); appearance of thorn-like appendages on the suspensors (S6); zygote formation by fusion of two gametangia ($7); and maturation of zygote to zygospore with the maximum size and a thick black coat ($8).…”

Section: The Heterothallic Fungus Phycomyces Blakesleeanusmentioning

confidence: 99%

“…The mating of Phycomyces is greatly influenced by medium composition as well as external conditions such as temperature, light, pH, moisture, and aeration (Robbins and Schmitt, 1945;Hocking, 1967;Sutter, 1975; ~The first and the second authors contributed equally to this work. The first author's present address: Photodynamics Research Center, The Institute of Physical and Chemical Research (PDC-RIKEN), Sendai 980-0868, Japan.…”

Section: The Heterothallic Fungus Phycomyces Blakesleeanusmentioning

confidence: 99%

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Effects of chemical components and nitrogen sources on zygospore development in Phycomyces blakesleeanus

et al. 2001

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We examined the effects of chemical components and nitrogen sources on zygospore development, using 62 different ingredients based on Sutter's synthetic medium SI, which has been widely used for studies of sexual physiology in Phycomyces, An increase of inorganic microelements such as ZnS04, NaMo04 and CaCI2 promoted an increase in the number of zygospores per unit area. Glutamate (Glu) contained in SI as the sole nitrogen source was indispensable for sexual development, and replacement of Glu with NH4+(Am) strongly inhibited it, mainly because of growth inhibition. However, zygospore production was enhanced 1.8-fold by equivalent amounts of both Glu and Am as compared with Glu alone. A newly developed medium, mSl+Am, enriched with Am and the above-mentioned effective microelements doubled the number of zygospores formed per unit area (density), compared with Sutter's original SI, and increased both the density and the weight (volume) of zygospores, 1.6-and 2-fold, respectively, compared with potatodextrose-agar medium enriched with yeast extract and casitone (PDAYC). Sexual stimulation by mSl+Am was also observed in the mating of a pair of ~-carotene-deficient mutants. Methionine sulfoxime, an inhibitor of glutamine synthetase, strongly inhibited the progress of mating without significant growth inhibition.

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Section: Discussionmentioning

confidence: 99%

Section: The Heterothallic Fungus Phycomyces Blakesleeanusmentioning

confidence: 99%

Section: The Heterothallic Fungus Phycomyces Blakesleeanusmentioning

confidence: 99%

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Effects of chemical components and nitrogen sources on zygospore development in Phycomyces blakesleeanus

et al. 2001

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“…This would explain the 1:1:2 ratio among (+), (-), and (+,-) zygospore cultures from the crosses between near isogenic parents, and account for the (+,-) zygospore cultures with frequency below 50% among zygospore progenies from the crosses between dissimilar parents, leading to the deviation from the 1:1:2 ratio (Tables 8 and 10). This may also explain the finding that the reported frequencies of mating type heterokaryons in the zygospore progenies of P. blakesleeanus varied from 0 to nearly 50% (Hocking 1967;Eslava et al 1975a). Our results show that near isogenic pair of C. cucurbitarum are an ideal pair of organisms for laboratory exercise in genetics class, as they are easy and fast to perform.…”

Section: No Of Nucleimentioning

confidence: 95%

The inheritance of mating type and growth rate inChoanephora cucurbitarum

Yu¹,

Ko²

1999

Can. J. Bot.

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When isolates of Choanephora cucurbitarum (Berk. & Rav.) Thaxt with similar growth rate and colony appearance were crossed, the mating-type distribution of zygospore progeny fit the ratio of 1(+):1(-):2(+,-). However, germsporangiospore progeny from the same pairs fit a 1(+):1(-) ratio but not a 1(+):1(-):2(+,-) ratio. When isolates with different growth type (F for fast and S for slow) were crossed, the resulting zygospore progenies fit a 1(+):1(-) ratio but not a 1(+):1(-):2(+,-) ratio. Their growth type distribution also fit a 1F:1S ratio. All the sporangiospores tested were multinucleate.

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“…13). A report by Hocking (1967) from studies of Phy comyces blakesleeanus shows that at least immature zygospores could be induced to undergo precocious germination with hyphal growth. These germinating zygospo res were, however, young, -only 2-4 days old and had not yet developed the thick, dark exospore.…”

Section: Sporangium Formationmentioning

confidence: 99%

Cytological studies on the life-cycle in Absidia glauca Hagem (Mucorales).

Duus

Laane

1984

CYTOLOGIA

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The life-cycle and nuclear conditions in Zygomycetes present still problems in spite of more than 100 years research. The process of conjugation was discovered as early as 1820 by Ehrenberg. In two papers from 1984 Dangeard and Leger made important observations on nuclear behaviour in the zygospores. Blakeslee (1904, 1906) examined different species, with the aim of establishing them as experimental material and to develop a field of fungal genetics. Unfortunately, nuclear behav iour both during formation, dormancy and germination of the zygospores remained obscure. Different problems arose, involving absence of regular germination of the zygospores and not at least, problems of staining and studying the tiny nuclei.

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Zygospore initiation, development and germination in Phycomyces blakesleeanus

Cited by 18 publications

References 16 publications

Effects of chemical components and nitrogen sources on zygospore development in Phycomyces blakesleeanus

Effects of chemical components and nitrogen sources on zygospore development in Phycomyces blakesleeanus

The inheritance of mating type and growth rate inChoanephora cucurbitarum

Cytological studies on the life-cycle in Absidia glauca Hagem (Mucorales).

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