Zygotorulaspora dagestanica sp. nov., a novel ascomycetous yeast species associated with the Georgian honeysuckle (Lonicera iberica M. Bieb.)

Abstract

During an investigation of the yeast communities associated with wild fruit shrubs in Dagestan (Caucasus, Russia), four fermenting ascospore-producing yeast strains were isolated from leaves of the Georgian honeysuckle (Lonicera iberica M. Bieb.) and from soil underneath this plant. Phylogenetic analyses based on concatenated sequences of the ITS region and D1/D2 domains of the large subunit rRNA gene and concatenated sequences of the ribosomal DNA cystron, RPB2 and TEF1 genes showed that the isolated strains represented a new species of the genus Zygotorulaspora. The new species was placed in the basal position to other species of the clade and close to Zygotorulaspora mrakii. Based on the results of phylogenetic analyses and the phenotypic characteristics of the four studied strains, a novel species is described, for which the name Zygotorulaspora dagestanica sp. nov. is proposed. The holotype is KBP Y-4591T, three metabolically inactive cryopreserved isotype cultures are DSM 100088, VKM Y-3060 and VKPM Y-4318. The MycoBank number is MB 838285.

Fungi under Modified Atmosphere—The Effects of CO2 Stress on Cell Membranes and Description of New Yeast Stenotrophomyces fumitolerans gen. nov., sp. nov.

“…The sub-clade containing genera Kazachstania, Yueomyces, Tetrapisispora and the novel yeast received moderate (bootstrap value of 70% for the ML tree) support in the analysis. The phylogenetic analysis was consistent with previous analyses by Kachalkin et al [33] and Shen et al [39]. The potential new yeast species was placed in the phylogenetic tree close to the genera Tetrapisispora and Yueomyces, although the support for the placement was weak (Figure 1).…”

“…For strain WT5, nucleotide sequence alignments were aligned in the dataset previously used by Kachalkin et al [33] in the description and placement of Zygotorulaspora dagestanica using the online version of the MAFFT algorithm [34]. Phylogenetic relationships in the concatenated alignment of the five loci were inferred by the Maximum-Likelihood (ML) method with RAxML GUI 2.0 [35] with 100 thorough bootstrap replicates.…”