Zymomonas mobilis immobilization in polymeric membranes for improved resistance to lignocellulose-derived inhibitors in bioethanol fermentation

Nguyen, Duong Thi; Praveen, Prashant; Loh, Kai‐Chee

doi:10.1016/j.bej.2018.09.003

Cited by 23 publications

(9 citation statements)

References 34 publications

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“…This method has advantages such as improved process design, a decrease in operating cost, the integration of the operating unit, higher productivity, and fewer byproducts . Furthermore, membrane separations are easy to scale up and also involve low energy requirements, since no phase change usually takes place in such processes . Therefore, the advantages of integrating enzyme immobilization and the membrane separation process makes the enzymatic membrane reactor (EMR) an appropriate alternative for dye removal.…”

Section: Introductionmentioning

confidence: 99%

Decolorization of dye solutions by tyrosinase in enzymatic membrane reactors

Veismoradi

Mousavi

Taherian

2019

J of Chemical Tech & Biotech

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BACKGROUND:The main aim of this study was to investigate the decolorization performance of enzymatic membrane reactors (EMRs) using tyrosinase, including direct contact membrane reactor (DCMR) and immobilized enzyme membrane reactor (IEMR). The crosslinked polyacrylonitrile (PAN)/chitosan composite membrane was employed in a separation unit as support for tyrosinase immobilization to decolorize two common Acid Blue 113 (AB113) and Direct Black 22 (DB22) azo dyes for the first time.RESULTS: According to the obtained results, the optimum enzyme amount, which could be immobilized on the membrane surface, was 25 units. The proper operating conditions for both DCMR and IEMR were determined as pH 6.5 and temperature 30 ∘ C. Both the reactors initially accounted for around 96% and 94% decolorization for AB113 and DB22, respectively. To determine the reusability of EMRs after 10 cycles, 83.1% and 78.5% dye removal were achieved using IEMR for AB133 and DB22, respectively; while for DCMR, this amount reduced to 60.5% and 58.3% for AB113 and DB22, respectively. CONCLUSION:The proposed IEMR maintained a high dye removal amount through the studied pH and temperature ranges. However, the performance of DCMR considerably reduced under the intensive operating conditions as well as in the long-term operation. As a result, the suggested immobilized enzyme showed superior and stable performance compared to the free one in the long-term operation.

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Section: Introductionmentioning

confidence: 99%

Decolorization of dye solutions by tyrosinase in enzymatic membrane reactors

Veismoradi

Mousavi

Taherian

2019

J of Chemical Tech & Biotech

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“…Compared with free‐cell fermentation, Zymomonas mobilis immobilized on the hollow fiber membrane exhibited good bacterial growth and glucose uptake rate in the presence of these inhibitors. The final ethanol production yield could reach 95% of the theoretical yield under conditions where inhibitors are highly concentrated 57 . The immobilized cells in the hollow fiber membrane bioreactor were also very stable, which could be operated for 20 consecutive times 57 .…”

Section: Application Of Polymer Membrane Materials In the Field Of Biological Fermentationmentioning

confidence: 99%

“…Membrane‐based immobilization does not require any covalent cross‐linking or ionic bonding, and cells can simply diffuse inside and outside the membrane 56 . Membrane‐mediated cell immobilization can be used in the microbial fermentation process under some harsh conditions, such as biofuel production from the undetoxified lignocellulosic hydrolysates 57 . It is well known that a single inhibitor of lignocellulosic hydrolytic products, including vanillin, syringa, 5‐hydroxymethylfurfural, and 4‐hydroxy‐3‐methoxy cinnamaldehyde is toxic to cells, while simultaneous exposure to multiple inhibitors is more toxic to cells 27 .…”

Section: Application Of Polymer Membrane Materials In the Field Of Biological Fermentationmentioning

confidence: 99%

“…It is well known that a single inhibitor of lignocellulosic hydrolytic products, including vanillin, syringa, 5‐hydroxymethylfurfural, and 4‐hydroxy‐3‐methoxy cinnamaldehyde is toxic to cells, while simultaneous exposure to multiple inhibitors is more toxic to cells 27 . Immobilized cells in hollow fiber membrane bioreactors can reduce the inhibitors’ toxicity to cells 57 . Compared with free‐cell fermentation, Zymomonas mobilis immobilized on the hollow fiber membrane exhibited good bacterial growth and glucose uptake rate in the presence of these inhibitors.…”

Section: Application Of Polymer Membrane Materials In the Field Of Biological Fermentationmentioning

confidence: 99%

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Material‐mediated cell immobilization technology in the biological fermentation proces

Gao

Jiang

et al. 2021

Biofuels Bioprod Bioref

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Cell immobilization technology has shown much potential in the field of biological fermentation owing to its simple operation, maintenance of long‐term cell viability, repeatability, good stability, and high tolerance. Compared with free‐cell fermentation, immobilized cells show strong growth and metabolism performance in complex environments, such as environments with low pH, or with high concentrations of substrate and product. The metabolic properties of immobilized cells mainly depend on the carrier materials. In recent years, polymer membrane materials have attracted much attention because of their good mechanical properties and flexible support structure. In addition to improving the activity and stability of immobilized cells, biocompatibility modification can also be carried out on the surface of the carrier, which can reduce specific non‐biological interactions between the cells and the carrier. In this review, current advances in cell immobilization using different materials in the field of biological fermentation are reviewed. Perspectives on immobilized cells and the challenges associated with them in the field of biological fermentation are also addressed. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…Bioethanol production from various carbohydrate-based substrates and wastes such as DFW has been explored by many researchers that have proposed different fermentation strategies via monocultures of bacteria or yeasts [5][6][7][8][9][10] but also co-cultures of yeasts, fungi, and bacteria [11][12][13][14][15]. Unlike other types of biomass, such as agricultural and forestry residues, prunings, olive mill wastes, etc.…”

Section: Introductionmentioning

confidence: 99%

Sustainable Second-Generation Bioethanol Production from Enzymatically Hydrolyzed Domestic Food Waste Using Pichia anomala as Biocatalyst

2020

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In the current study, a domestic food waste containing more than 50% of carbohydrates was assessed as feedstock to produce second-generation bioethanol. Aiming to the maximum exploitation of the carbohydrate fraction of the waste, its hydrolysis via cellulolytic and amylolytic enzymatic blends was investigated and the saccharification efficiency was assessed in each case. Fermentation experiments were performed using the non-conventional yeast Pichia anomala (Wickerhamomyces anomalus) under both separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) modes to evaluate the conversion efficiencies and ethanol yields for different enzymatic loadings. It was shown that the fermentation efficiency of the yeast was not affected by the fermentation mode and was high for all handlings, reaching 83%, whereas the enzymatic blend containing the highest amount of both cellulolytic and amylolytic enzymes led to almost complete liquefaction of the waste, resulting also in ethanol yields reaching 141.06 ± 6.81 g ethanol/kg waste (0.40 ± 0.03 g ethanol/g consumed carbohydrates). In the sequel, a scale-up fermentation experiment was performed with the highest loading of enzymes in SHF mode, from which the maximum specific growth rate, μmax, and the biomass yield, Yx/s, of the yeast from the hydrolyzed waste were estimated. The ethanol yields that were achieved were similar to those of the respective small scale experiments reaching 138.67 ± 5.69 g ethanol/kg waste (0.40 ± 0.01 g ethanol/g consumed carbohydrates).

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Zymomonas mobilis immobilization in polymeric membranes for improved resistance to lignocellulose-derived inhibitors in bioethanol fermentation

Cited by 23 publications

References 34 publications

Decolorization of dye solutions by tyrosinase in enzymatic membrane reactors

Decolorization of dye solutions by tyrosinase in enzymatic membrane reactors

Material‐mediated cell immobilization technology in the biological fermentation proces

Sustainable Second-Generation Bioethanol Production from Enzymatically Hydrolyzed Domestic Food Waste Using Pichia anomala as Biocatalyst

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