Zymomonas mobilis—Towards Bacterial Biofuel

Clarke, Kim Gail; Mokomele, Thapelo; Callanan, Linda H.; Groenewald, J. W.

doi:10.1007/978-3-319-63612-2_13

Cited by 2 publications

(2 citation statements)

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“…The overall ethanol productivity of each cycle was kept between 2.4 and 2.6 g L –1 h –1 after six cycles (Figure 4). This was higher than the study reported by Kim et al, who used Z. mobilis 8b for fermentation at sugar concentration of 100 g/L and obtained 1.43 g L –1 h –1 (Clarke et al, 2018). The increased ethanol productivity was related with the high cell density fermentation with cell recycling, which can accelerate fermentation in the reactor (Santos et al, 2016).…”

Section: Resultscontrasting

confidence: 59%

“…However, our previous studies focused on the improvement of fermentation efficiencies of Saccharomyces cerevisiae , while other commonly used fermenting microorganisms were not assessed. Compared with S. cerevisiae , Zymomonas mobilis 8b has been reported to exhibit excellent ethanol productivity, high ethanol tolerance and efficient sugar uptake (Rogers et al, 2007; Clarke et al, 2018; Xia et al, 2019). Recently, many studies have engineered Z. mobilis strains to enable it to utilize both five-carbon and six-carbon sugars (Yang S. et al, 2018; Yang Y. et al, 2018; Yang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Boosting Ethanol Productivity of Zymomonas mobilis 8b in Enzymatic Hydrolysate of Dilute Acid and Ammonia Pretreated Corn Stover Through Medium Optimization, High Cell Density Fermentation and Cell Recycling

Li¹,

Zhai²,

Jiang³

et al. 2019

Front. Microbiol.

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The presence of toxic degradation products in lignocellulosic hydrolysate typically reduced fermentation rates and xylose consumption rate, resulting in a decreased ethanol productivity. In the present study, Zymomonas mobilis 8b was investigated for high cell density fermentation with cell recycling to improve the ethanol productivity in lignocellulosic hydrolysate. The fermentation performances of Z. mobilis 8b at various conditions were first studied in yeast extract-tryptone medium. It was found that nutrient level was essential for glucose and xylose co-fermentation by Z. mobilis 8b and high cell density fermentation with cell recycling worked well in yeast extract-tryptone medium for 6 rounds fermentation. Z. mobilis 8b was then studied in enzymatic hydrolysates derived from dilute acid (DA) pretreated corn stover (CS) and ammonia pretreated CS for high cell density fermentation with cell recycling. Ethanol productivity obtained was around three times higher compared to traditional fermentation. Ethanol titer and metabolic yield were also enhanced with high cell density fermentation. Z. mobilis 8b cells showed high recyclability in ammonia pretreated CS hydrolysate.

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Section: Resultscontrasting

confidence: 59%