Zymomonas mobilis ZM4 Utilizes an NADP
            <sup>+</sup>
            -Dependent Acetaldehyde Dehydrogenase To Produce Acetate

Felczak, Magdalena M.; TerAvest, Michaela A.; Maupin-Furlow, ed.

doi:10.1128/jb.00563-21

Cited by 12 publications

(4 citation statements)

References 26 publications

(41 reference statements)

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“…The negative effect of oxygen on Z. mobilis has been attributed to accumulation of the toxic metabolic intermediate acetaldehyde (6, 7). While some of the acetaldehyde is converted to acetate by an acetaldehyde dehydrogenase, residual acetaldehyde is often observed in aerobic cultures of Z. mobilis (8).…”

Section: Introductionmentioning

confidence: 99%

Respiration is essential for aerobic growth ofZymomonas mobilisZM4

Felczak

TerAvest

2023

Preprint

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Zymomonas mobilisis an alpha-proteobacterium that is a promising platform for industrial scale production of biofuels or valuable products due to its efficient ethanol fermentation and low biomass generation.Z. mobilishas also intriguing physiology, sometimes difficult to explain by the rules and strategies commonly observed in other bacteria. One of the most mysterious features ofZ. mobilisis its growth in oxic conditions.Z. mobilisis an aerotolerant bacterium that encodes a complete respiratory electron transport chain but the benefit of respiration for growth in oxic conditions has never been confirmed, despite decades of research. Quite the opposite, growth and ethanol production of WTZ. mobilisis poor in oxic conditions indicating that it does not benefit from oxidative phosphorylation. Additionally, in previous studies, aerobic growth improved significantly when respiratory genes were disrupted (ndh) or acquired point mutations (cydA, cydB)even if respiration was significantly reduced by these changes. Here, we obtained clean deletions of respiratory genesndhandcydAB, individually and in combination, and showed, for the first time, that deletion ofcydABcompletely inhibited O2respiration and dramatically reduced growth in oxic conditions. Both respiration and aerobic growth were restored by expressing a heterologous, water-forming NADH oxidase,noxE. This result shows that the main role of the electron transport chain inZ. mobilisis reducing the toxicity of molecular oxygen, helping to explain why it is beneficial forZ. mobilisto use electron transport chain complexes that contribute little to oxidative phosphorylation.ImportanceA key to producing next generation biofuels is to engineer microbes that efficiently convert non-food materials into drop-in fuels and to engineer microbes effectively we must understand their metabolism thoroughly.Zymomonas mobilisis a bacterium that is a promising candidate biofuel producer but its metabolism remains poorly understood, especially its metabolism when exposed to oxygen. AlthoughZ. mobilisrespires with oxygen, its aerobic growth is poor and disruption of genes related to respiration counterintuitively improves aerobic growth. This unusual result has sparked decades of research and debate regarding the function of respiration inZ. mobilis. Here, we used a new set of mutants to determine that respiration is essential for aerobic growth and likely protects the cells from oxidative damage caused by molecular oxygen. These results indicate that respiration has a non-canonical function inZ. mobilisand expand our understanding of the role of respiration in metabolism and oxidative stress.

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Section: Introductionmentioning

confidence: 99%

Respiration is essential for aerobic growth ofZymomonas mobilisZM4

Felczak

TerAvest

2023

Preprint

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“…Although the latest published model i ZM4_478 has improved GPR association through pooled transposon mutant fitness experiments [ 44 ], i ZM516 further corrected GPR association based on experiment results such as 13 C-labelled metabolomics data [ 39 ] and the latest literature, such as ZMO1754 encoding an NADP + -dependent acetaldehyde dehydrogenase that was determined recently [ 45 ]. All manual curation reaction list can be found in supplementary materials ( Supplementary file 2 ).…”

Section: Resultsmentioning

confidence: 99%

Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories

Yuan

Yang

et al. 2023

Synthetic and Systems Biotechnology

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“…Another potential detoxifying agent present in the arsenal of P. pachyrhizi is the effector jgi_PhapaK8108_3062326, which we identified as having Aldehyde/histidinol dehydrogenase activity with a molecular oxidation reductase function. Aldehyde dehydrogenase, as well as SOD, are collectors of oxidative species present in the medium (Felczak;TerAvest, 2022). Gene deletion in Magnaporthe oryzae impacted pathogenicity (Abdul et al, 2018).…”

Section: Functional Annotation and Carbohydrate-active Enzymes (Cazym...mentioning

confidence: 99%

Deciphering the Soybean Rust Arsenal: Effectorome Studies and Host-Pathogen Interaction (HPI) network inPhakopsora pachyrhiziandGlycine maxPathosystem

Oliveira,

Pereira

2024

Preprint

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Asian soybean rust (ASR), cause by the fungal pathogenPhakopsora pachyrhizi, stands out as the paramount menace to soybean cultivation, resulting in staggering yield decreases that can surpass 90%. Despite extensive endeavors, the development of cultivars endowed with comprehensive or enduring resistance against the myriad pathotypes remains elusive, thereby underscoring the imperative for pioneering improvement tactics. Fungal effectors, orchestrating the infiltration and colonization of hosts, constitute pivotal determinants in the infection cascade, thus emerging as prime targets for unraveling pathogenicity mechanisms. In this investigation, we endeavored to integrate effectoromics analysis with host-pathogen protein-protein interaction networks (HPPI) to delineate the modus operandi ofP. pachyrhizieffectors in subverting plant host immunity. By meticulously delineating the repertoire of predicted effectors and prognosticating protein-protein interactions between effectors and soybean target proteins, we discerned pivotal candidate targets. Notably, a repertoire of 324 host proteins was anticipated to engage in interactions with 21 effectors, predominantly hailing from the Lysophospholipase (LysoPLs) cohort. Particularly conspicuous was the identification of a robust effector candidate (PhapaK8108_7408047) implicated in the targeting of 171 soybean proteins. Its functional involvement in RNA splicing, chromatin organization, histone deacetylation, protein stabilization, and protein folding signifies its pivotal role in the infectious process. Furthermore, our endeavor encompassed the categorization of effectors into carbohydrate-active families, illuminating the prominence of Carbohydrate esterases family 12, primarily catalyzing cellulose and xylan degradation. By amalgamating these multifaceted methodologies, our study provides invaluable insights leading to the formulation of more effective ASR molecular strategies, demonstrating its relevance and innovation in the fields of plant pathology and molecular biology.

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Zymomonas mobilis ZM4 Utilizes an NADP ⁺ -Dependent Acetaldehyde Dehydrogenase To Produce Acetate

Cited by 12 publications

References 26 publications

Respiration is essential for aerobic growth ofZymomonas mobilisZM4

Respiration is essential for aerobic growth ofZymomonas mobilisZM4

Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories

Deciphering the Soybean Rust Arsenal: Effectorome Studies and Host-Pathogen Interaction (HPI) network inPhakopsora pachyrhiziandGlycine maxPathosystem

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Zymomonas mobilis ZM4 Utilizes an NADP + -Dependent Acetaldehyde Dehydrogenase To Produce Acetate

Cited by 12 publications

References 26 publications

Respiration is essential for aerobic growth ofZymomonas mobilisZM4

Respiration is essential for aerobic growth ofZymomonas mobilisZM4

Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories

Deciphering the Soybean Rust Arsenal: Effectorome Studies and Host-Pathogen Interaction (HPI) network inPhakopsora pachyrhiziandGlycine maxPathosystem

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Zymomonas mobilis ZM4 Utilizes an NADP ⁺ -Dependent Acetaldehyde Dehydrogenase To Produce Acetate