ZYX-1, the unique zyxin protein of<i>Caenorhabditis elegans</i>, is involved in dystrophin-dependent muscle degeneration

Lecroisey, Claire; Brouilly, Nicolas; Qadota, Hiroshi; Mariol, Marie Christine; Rochette, Nicolas C.; Martin, Edwige; Benian, Guy M.; Ségalat, Laurent; Mounier, Nicole; Gieseler, Kathrin

doi:10.1091/mbc.e12-09-0679

Cited by 31 publications

(56 citation statements)

References 103 publications

(103 reference statements)

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“…Prior work in C. elegans has examined the role of zyx-1 in muscle (Lecroisey et al, 2008(Lecroisey et al, , 2013. As mentioned earlier, ZYX-1 is localized to dense bodies in muscle and this localization is ATN-1 dependent.…”

Section: Discussionmentioning

confidence: 91%

“…Zyxin has been demonstrated to interact with focal adhesions via α-actinin (Reinhard et al, 1999). Furthermore, prior studies revealed that zyx-1 muscle phenotypes (in a dys-1 hlh-1 mutant background) are mediated via the worm α-actinin ATN-1 (Lecroisey et al, 2013). However, atn-1 mutants formed PLM synapses normally ( Table 1), indicating that ZYX-1 functions via different interacting proteins in muscle versus PLM neurons.…”

Section: Developmental Studies Confirm Synaptic Lossmentioning

confidence: 98%

“…ZYX-1B functions cell-autonomously in PLMs to regulate synapse development zyx-1 is a complex gene that is expressed as multiple different isoforms in both muscle and neurons (Lecroisey et al, 2013) (supplementary material Fig. S7).…”

Section: Developmental Studies Confirm Synaptic Lossmentioning

confidence: 99%

“…Together, our results show that the second and third ZYX-1 LIM domains act cell-autonomously to regulate mechanosensory synapse development. Lecroisey et al, 2008Lecroisey et al, , 2013. To define potential mechanisms of action for zyx-1 in mediating PLM synapse development, we examined C. elegans mutants in which these components are disrupted.…”

Section: Developmental Studies Confirm Synaptic Lossmentioning

confidence: 99%

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The conserved LIM domain-containing focal adhesion protein ZYX-1 regulates synapse maintenance inCaenorhabditis elegans

et al. 2014

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We describe the identification of zyxin as a regulator of synapse maintenance in mechanosensory neurons in C. elegans. zyx-1 mutants lacked PLM mechanosensory synapses as adult animals. However, most PLM synapses initially formed during development but were subsequently lost as the animals developed. Vertebrate zyxin regulates cytoskeletal responses to mechanical stress in culture. Our work provides in vivo evidence in support of such a role for zyxin. In particular, zyx-1 mutant synaptogenesis phenotypes were suppressed by disrupting locomotion of the mutant animals, suggesting that zyx-1 protects mechanosensory synapses from locomotion-induced forces. In cultured cells, zyxin is recruited to focal adhesions and stress fibers via C-terminal LIM domains and modulates cytoskeletal organization via the N-terminal domain. The synapse-stabilizing activity was mediated by a short isoform of ZYX-1 containing only the LIM domains. Consistent with this notion, PLM synaptogenesis was independent of α-actinin and ENA-VASP, both of which bind to the N-terminal domain of zyxin. Our results demonstrate that the LIM domain moiety of zyxin functions autonomously to mediate responses to mechanical stress and provide in vivo evidence for a role of zyxin in neuronal development.

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Section: Discussionmentioning

confidence: 91%

Section: Developmental Studies Confirm Synaptic Lossmentioning

confidence: 98%

Section: Developmental Studies Confirm Synaptic Lossmentioning

confidence: 99%

Section: Developmental Studies Confirm Synaptic Lossmentioning

confidence: 99%

See 2 more Smart Citations

The conserved LIM domain-containing focal adhesion protein ZYX-1 regulates synapse maintenance inCaenorhabditis elegans

et al. 2014

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“…Strains that overexpress a given transgene are useful tools to study i) the subcellular localization of proteins, which exhibit a low endogenous expression level, ii) the effect of the overexpression of a protein on worm physiology, or iii) the effect of the overexpression of a protein in a particular tissue 11 . Several applications require however the use of low or single copy integrated transgenes that can be generated with alternative methods such as coinjection of the transgene of interest along with single stranded oligos 2 , bombardment 12 , Mos1 transposon mediated Single Copy gene Insertion (MosSCI) 13 or Cas-9-triggered homologous recombination 14 .…”

Section: Discussionmentioning

confidence: 99%

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the <em>C. elegans</em> Genome

Mariol

Walter

Bellemin

et al. 2013

JoVE

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Microinjecting DNA into the cytoplasm of the syncytial gonad of Caenorhabditis elegans is the main technique used to establish transgenic lines that exhibit partial and variable transmission rates of extrachromosomal arrays to the next generation. In addition, transgenic animals are mosaic and express the transgene in a variable number of cells. Extrachromosomal arrays can be integrated into the C. elegans genome using UV irradiation to establish nonmosaic transgenic strains with 100% transmission rate of the transgene. To that extent, F1 progenies of UV irradiated transgenic animals are screened for animals carrying a heterozygous integration of the transgene, which leads to a 75% Mendelian transmission rate to the F2 progeny. One of the challenges of this method is to distinguish between the percentage of transgene transmission in a population before (X% transgenic animals) and after integration (≥75% transgenic F2 animals). Thus, this method requires choosing a nonintegrated transgenic line with a percentage of transgenic animals that is significantly lower than the Mendelian segregation of 75%. Consequently, nonintegrated transgenic lines with an extrachromosomal array transmission rate to the next generation ≤60% are usually preferred for integration, and transgene integration in highly transmitting strains is difficult. Here we show that the efficiency of extrachromosomal arrays integration into the genome is increased when using highly transmitting transgenic lines (≥80%). The described protocol allows for easy selection of several independent lines with homozygous transgene integration into the genome after UV irradiation of transgenic worms exhibiting a high rate of extrachromosomal array transmission. Furthermore, this method is quite fast and low material consuming. The possibility of rapidly generating different lines that express a particular integrated transgene is of great interest for studies focusing on gene expression pattern and regulation, protein localization, and overexpression, as well as for the development of subcellular markers. Video LinkThe video component of this article can be found at

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FLN‐1/filamin is required to anchor the actomyosin cytoskeleton and for global organization of sub‐cellular organelles in a contractile tissue

et al. 2020

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Actomyosin networks are organized in space, direction, size, and connectivity to produce coordinated contractions across cells. We use the C. elegans spermatheca, a tube composed of contractile myoepithelial cells, to study how actomyosin structures are organized. FLN-1/filamin is required for the formation and stabilization of a regular array of parallel, contractile, actomyosin fibers in this tissue. Loss of fln-1 results in the detachment of actin fibers from the basal surface, which then accumulate along the cell junctions and are stabilized by spectrin. In addition, actin and myosin are captured at the nucleus by the linker of nucleoskeleton and cytoskeleton complex (LINC) complex, where they form large foci. Nuclear positioning and morphology, distribution of the endoplasmic reticulum and the mitochondrial network are also disrupted. These results demonstrate that filamin is required to prevent large actin bundle formation and detachment, to prevent excess nuclear localization of actin and myosin, and to ensure correct positioning of organelles.

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ZYX-1, the unique zyxin protein ofCaenorhabditis elegans, is involved in dystrophin-dependent muscle degeneration

Cited by 31 publications

References 103 publications

The conserved LIM domain-containing focal adhesion protein ZYX-1 regulates synapse maintenance inCaenorhabditis elegans

The conserved LIM domain-containing focal adhesion protein ZYX-1 regulates synapse maintenance inCaenorhabditis elegans

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the <em>C. elegans</em> Genome

FLN‐1/filamin is required to anchor the actomyosin cytoskeleton and for global organization of sub‐cellular organelles in a contractile tissue

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