α‐Amylase and cellulase production by novel halotolerant <i>Bacillus</i> sp.PM06 isolated from sugarcane pressmud

Rajesh, Rekha; Gummadi, Sathyanarayana N.

doi:10.1002/bab.2091

Cited by 8 publications

(5 citation statements)

References 46 publications

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“…Similar findings were observed in Bacillus sp. PM06 [65]. In another study, maximum production of cellulase was observed at pH 9 by Bacillus sp.…”

Section: Cellulase Production and Characterization Effect Of Ph On Ce...mentioning

confidence: 92%

“…A similar outcome was discovered in Bacillus sp. PM06 [65]. Maximum cellulase production was reached within 72 h for both Streptomyces sp.…”

Section: Effect Of the Incubation Periodmentioning

confidence: 96%

“…Similar findings were reported for Bacillus sp. PM06 [65]. In contrast, Pseudomonas fluorescens and Bacillus subtilis had maximum cellulase production when glucose was used as a carbon source [76].…”

Section: Effect Of Carbon and Nitrogen Sourcementioning

confidence: 99%

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Screening and Production of Industrially Relevant Enzymes by Bacillus paranthracis Strain MHDS3, a Potential Probiotic

Tshisikhawe,

Diale,

Abrahams

et al. 2023

Fermentation

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The digestive process and intestinal protein absorption are influenced by a variety of factors. Due to their numerous health advantages, including potential favorable effects on protein digestion and absorption, probiotics have gained increased attention in recent years. Probiotics can control the intestinal microflora, which in turn affects the intestinal bacteria responsible for proteolysis. Additionally, certain probiotics can release exoenzymes that aid in the digestion of proteins and others can stimulate the host’s digestive protease and peptidase activity. By boosting transport and enhancing the epithelium’s capacity for absorption, probiotics can also improve the absorption of tiny peptides and amino acids as well as lessen detrimental protein fermentation, which lowers the toxicity of metabolites. The present study explored the production of enzymes by Bacillus paranthracis strain MHDS3, a probiotic candidate isolated from Pellaea calomelanos. Bacillus paranthracis displayed enzyme activities of amylase (31,788.59 IU), cellulase (4487.486 IU), and pectinase (13.98986 IU) through submerged fermentation. The CAZyme analysis of B. paranthracis revealed 16 CAZyme gene clusters associated with cellulose, amylase, and pectinase activity. Thus, B. paranthracis is a promising probiotic strain that can produce enzymes with biotechnological applications.

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“…Similar findings were observed in Bacillus sp. PM06 [65]. In another study, maximum production of cellulase was observed at pH 9 by Bacillus sp.…”

Section: Cellulase Production and Characterization Effect Of Ph On Ce...mentioning

confidence: 92%

“…A similar outcome was discovered in Bacillus sp. PM06 [65]. Maximum cellulase production was reached within 72 h for both Streptomyces sp.…”

Section: Effect Of the Incubation Periodmentioning

confidence: 96%

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Screening and Production of Industrially Relevant Enzymes by Bacillus paranthracis Strain MHDS3, a Potential Probiotic

Tshisikhawe,

Diale,

Abrahams

et al. 2023

Fermentation

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“…Although mutants have been described in the literature regarding SecYEG components [10,[14][15][16], we use a different approach based on protein-protein interactions for the SecA proteins of the SecYEG system, TasA and TapA [12]. Tey are involved in the formation of bioflms and secretion of AmyE and AprE, which represent, respectively, amylase [17] and proteases [18][19][20][21][22]. For this work, recombinant proteins were fused with targeted proteins.…”

Section: Introductionmentioning

confidence: 99%

Involvement of the Bacillus SecYEG Pathway in Biosurfactant Production and Biofilm Formation

Okouakoua,

Kayath,

Mokemiabeka

et al. 2024

International Journal of Microbiology

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With Bacillus species, about 30% of extracellular proteins are translocated through the cytoplasmic membrane, coordinated by the Sec translocase. This system mainly consists of the cytoplasmic ATPase SecA and the membrane-embedded SecYEG channel. The purpose of this work was to investigate the effects of the SecYEG export system on the production of industrial biomolecules, such as biosurfactants, proteases, amylases, and cellulases. Fifty-two isolates of Bacillus species were obtained from traditional fermented foods and then characterized using molecular microbiology methods. The isolates secreted exoenzymes that included cellulases, amylases, and proteases. We present evidence that a biosurfactant-like molecule requires the SecA ATPase and the SecYEG membrane channel for its secretion. In addition, we showed that biomolecules involved in biofilm formation required the SecYEG pathway. This work presents a novel seven-target fragment multiplex PCR assay capable of identification at the species level of Bacillus through a unique SecDF chromosomal gene. The bacterial membrane protein SecDF allowed the discrimination of Bacillus subtilis, B. licheniformis, B. amyloliquefaciens, and B. sonorensis. SecA was able to interact with AprE, AmyE, and TasA. The Rose Bengal inhibitor of SecA crucially affected the interaction of AprE, AmyE, TapA, and TasA with recombinant Gst-SecA. The Rose Bengal prevented Bacillus species from secreting and producing proteases, cellulases, amylases, and biosurfactant-like molecules. It also inhibited the formation of biofilm cell communities. The data support, for the first time, that the SecYEG translocon mediates the secretion of a biosurfactant-like molecule.

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“…When salt is used as a preservation agent, halotolerant bacteria may contaminate the drysalted and brine-salted food products. In previous studies, halotolerant bacteria were isolated from salted fish [10], fish sauce [11], cheese brine [12], fermented food [13], salt crystal [14], sugarcane press mud [15], grasshopper sub shrimp paste [16], cheese rinds [17], and fermented Solanum macrocarpon food [18].…”

Section: Introductionmentioning

confidence: 99%

Gıda Tuzlarında Halotolerant Bakterilerin İncelenmesi

Birbir

HELVA

Çağlayan

2022

International Journal of Advances in Engineering and Pure Sciences

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Twenty-five food salts, collected from seven-country, were examined for moisture contents and ash contents, pH values, total numbers of bacteria, and total numbers of bacteria halotolerant bacteria. The growth and biochemical reactions of halotolerant bacteria at different NaCl concentrations, pH, and temperature values were investigated. The efficacy of dry-heat sterilization and high-pressure steam sterilization for the inactivation of bacteria was examined using six food salts. Moisture contents, ash contents, and pH values of salts were respectively found as 2.3-3.7%, 95-97%, and 6.37-9.94. Total numbers of bacteria and halotolerant bacteria were detected as between 1-58 CFU/g and 1-40 CFU/g on Modified Nutrient Agar media, respectively. While bacteria were found in twenty salt samples, halotolerant bacteria were detected in seventeen samples. One hundred thirty- eight rod-shaped halotolerant bacteria were isolated from seventeen samples. One hundred eighteen and twenty isolates were found Gram-positive and Gram-negative, respectively. While 131 isolates were motile, seven isolates were observed as non-motile. All isolates grew in both absence of NaCl and presence of 10% NaCl, pH 5, 7, and 9, 24°C, and 37°C. Eighty-six isolates showed different pigmentations. Ammonia production was detected at sixty isolates. Catalase, oxidase, protease, lipase, amylase, caseinase were produced by 138, 83, 84, 48, 92, 100 isolates, respectively. Fifty-seven percent of halotolerant bacteria formed endospores. All bacteria in six salts were killed using both dry-heat sterilization (175oC for 2 hours) and high-pressure steam sterilization (121oC for 30 minutes). In conclusion, we suggest using dry-heat sterilization or high-pressure steam sterilization to kill Gram-negative halotolerant bacteria containing endotoxin and Gram-positive halotolerant bacteria with endospores in food salts to prevent food spoilage and foodborne diseases in humans.

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α‐Amylase and cellulase production by novel halotolerant Bacillus sp.PM06 isolated from sugarcane pressmud

Cited by 8 publications

References 46 publications

Screening and Production of Industrially Relevant Enzymes by Bacillus paranthracis Strain MHDS3, a Potential Probiotic

Screening and Production of Industrially Relevant Enzymes by Bacillus paranthracis Strain MHDS3, a Potential Probiotic

Involvement of the Bacillus SecYEG Pathway in Biosurfactant Production and Biofilm Formation

Gıda Tuzlarında Halotolerant Bakterilerin İncelenmesi

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