Α-Glucosidase INHIBITION ACTIVITY OF STANDARDIZED EXTRACT OF ALOE VERA COLLECTED FROM YAYO BIOSPHERE RESERVE FOREST, ETHIOPIA

Abstract

In recent years, there has been an increased interest in the development of alternative medicines for maintaining glucose homeostasis in diabetes, specifically by screening plant extracts as well as their isolated compounds for their ability to delay or prevent glucose metabolism and absorption. The objective of present study was to evaluate the in-vitro α-glucosidase inhibitory activity of standardized extract of Aloe vera. In this study, we standardized ethanolic extracts of Aloe vera by using thin layer chromatography (TLC) and UV-Visible spectroscopy and then standardized extract was selected for in-vitro α-glucosidase inhibitory assay. Standardized ethanolic extract of Aloe vera showed concentration dependent inhibition of αglucosidase varying from 13.96±0.67 to 76.10%±0.84 % for 100 to 1000 µg/mL, respectively. The IC50 values for Aloe vera extract and acarbose were found as 530±7.18 and 265±5.31 µg/ mL, respectively. The present study indicated the potential of Aloe vera to prevent postprandial hyperglycemia by inhibition of α-glucosidase enzyme.