α-Glucosidase, Sperm ATP Concentrations, and Epididymal Function

Fourie, M. H.; Toit, D. Du; Bornman, M. S.; Merwe, M. P. Van Der; Plessis, Du

doi:10.3109/01485019108987636

Cited by 23 publications

(10 citation statements)

References 7 publications

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“…This correlation is in agreement with Tremblay et al. (1979) and Fourie et al. (1991) who found that seminal plasma α ‐1,4‐glucosidase‐specific activity of semen correlated with sperm motility and that an abnormal function of the epididymis might be expected with a decreased production of this enzyme.…”

Section: Discussionsupporting

confidence: 91%

?-1,4-Glucosidase activity in infertile oligoasthenozoospermic men with and without varicocele

et al. 2007

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This work aimed to assess seminal alpha-1,4-glucosidase activity in infertile oligoasthenozoospermic men associated with and without scrotal varicocele. Eighty men were investigated. They were divided into three groups: group 1 (n = 20), fertile normozoospermic men; group 2 (n = 30), oligoasthenozoospermia with varicocele; and group 3 (n = 30), oligoasthenozoospermia without varicocele. The patients underwent medical history, clinical examination, conventional semen analysis and estimation of seminal plasma alpha-1,4-glucosidase activity by double-beam spectrophotometer method and serum testosterone by radioimmunoassay method. There was a significant decrease in the mean seminal alpha-1,4-glucosidase activity levels in infertile men versus controls (mean +/- SD; 7.66 +/- 0.433, 2.088 +/- 0.565, 5.384 +/- 0.85 mU ml(-1) respectively). Mean serum testosterone levels demonstrated nonsignificant differences between studied groups. Seminal alpha-1,4-glucosidase activity levels demonstrated significant correlation with sperm count, sperm motility percentage and serum testosterone in oligoasthenozoospermia with varicocele group and demonstrated nonsignificant correlation in other groups. It is concluded that varicocele-induced hypoxia is the adverse effect that causes both oligoasthenozoospermia and decreased seminal alpha-1,4-glucosidase levels.

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Section: Discussionsupporting

confidence: 91%

?-1,4-Glucosidase activity in infertile oligoasthenozoospermic men with and without varicocele

et al. 2007

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“…Functional competence is acquired during passage through the epididymal lumen [Dacheux et al 2003]. In a multiple regression analysis model, a positive significant association between NAG and percentage progressive motile spermatozoa was observed in this study, as reported by others [Fourie et al 1991;Viljoen et al 1990], indicating the importance of this organ for sperm maturation. The present study demonstrated decreased total activity of NAG among men over 50 years of age compared to younger men.…”

Section: Discussionsupporting

confidence: 67%

Association between Age and Epididymal and Accessory Sex Gland Function and their Relation to Sperm Motility

Elzanaty

2007

Archives of Andrology

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Increased male age has been associated with significant reduction in pregnancy rates. This study investigated the association between age, the function of epididymal and accessory sex glands, and their relation to sperm motility. Ejaculates from 498 men assessed for infertility were analysed according to WHO [1999] guidelines. Seminal markers of epididymal (neutral a-glucosidase (NAG)), prostatic (prostate-specific antigen (PSA) and zinc), and seminal vesicle function (fructose) were measured. Four groups according to age were defined: G 21-30 (21-30 years), G 31-40 (31-40 years), G 41-50 (41-50 years), and G > 50 (51-66 years). Percentage progressive motility was significantly lower in G > 50 compared with G 21-30 . NAG, PSA, zinc, and fructose were significantly lower in G > 50 compared with G 21-30 . In a multiple regression analysis model, NAG and PSA showed positive significant association with percentage progressive motility. The opposite trend was found regarding zinc. No association between fructose and percentage progressive motility was shown. In this cross-sectional study, declined sperm motility observed in men over 50 years of age might be due to age-dependent changes in epididymal and accessory sex gland function.

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“…Thus, the more the amount of sperm was, the higher the concentration of alpha-glucosidase in epididymis. There was a correlation between the level of alpha-glucosidase and sperm motility, especially significant correlation between the level of alpha-glucosidase and the sperm progressive motility [7][8][9]11]. However, our data showed that there was no any apparent correlation between the alpha-glucosidase activity in seminal plasma and total motile sperm or grades a and b motile sperm, similar to some other studies [11].…”

Section: Discussionsupporting

confidence: 87%

The Effect of Chymotrypsin on the Determination of Total Alpha-Glucosidase Activity in Seminal Plasma and the Correlation Between Alpha-Glucosidase Level and Semen Parameters

Xu¹,

Lü²,

Chen³

et al. 2006

Archives of Andrology

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& To evaluate the effect of chymotrypsin on the examination of alpha-glucosidase activity in seminal plasma, thirty-nine samples of fresh liquefied semen with or without chymotrypsin and forty-eight samples of fresh un-liquefied semen with chymotrypsin were determined for the total alpha-glucosidase activity in seminal plasma. The total alpha-glucosidase level of each sample was assayed by the method of glucose oxidase. The correlations between alpha-glucosidase level and semen parameters, including semen volume, pH, sperm concentration, grade a and b motility and total motility, were analyzed with SPSS 11.0 software. The results showed that chymotrypsin had no effect on seminal alpha-glucosidase activity determination. Chymotrypsin could improve the liquefaction for un-liquefied semen, and there was no significant difference of alpha-glucosidase activity between liquefied and un-liquefied semen samples. There were significantly positive correlations between seminal alpha-glucosidase activity (U=ml) and sperm concentration (r ¼ 0.338, p ¼ 0.015) and between total alpha-glucosidase activity (U=ejaculate) and semen volume (r ¼ 0.677, p ¼ 0.000). However, there was no significant correlation between alpha-glucosidase level (U=ml) and semen volume, pH, sperm motility or grade a and b motility (r ¼ À0.234 $ 0.077, p ¼ 0.099 $ 0.993). The data indicated that chymotrypsin could be added into the Address correspondence to Dr. Jin-Chun Lu, Department of Reproduction and Genetics, Nanjing General Hospital, PLA, 305 East Zhongshan Road, Nanjing 210002, P. R. China. E-mail: jclu@jlonline. un-liquefied semen samples for alpha-glucosidase activity determination, and there were different correlations between seminal alpha-glucosidase level and various semen parameters.

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α-Glucosidase, Sperm ATP Concentrations, and Epididymal Function

Cited by 23 publications

References 7 publications

?-1,4-Glucosidase activity in infertile oligoasthenozoospermic men with and without varicocele

?-1,4-Glucosidase activity in infertile oligoasthenozoospermic men with and without varicocele

Association between Age and Epididymal and Accessory Sex Gland Function and their Relation to Sperm Motility

The Effect of Chymotrypsin on the Determination of Total Alpha-Glucosidase Activity in Seminal Plasma and the Correlation Between Alpha-Glucosidase Level and Semen Parameters

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