α-Klotho Expression in Mouse Tissues Following Acute Exhaustive Exercise

Abstract

α-Klotho, a multifunctional protein, has been demonstrated to protect tissues from injury via anti-oxidation and anti-inflammatory effects. The expression of α-klotho is regulated by several physiological and pathological factors, including acute inflammatory stress, oxidative stress, hypertension, and chronic renal failure. Exhaustive exercise has been reported to result in tissue damage, which is induced by inflammation, oxidative stress, and energy metabolism disturbance. However, little is known about the effects of exhaustive exercise on the expression of α-klotho in various tissues. To determine the effects, the treadmill exhaustion test in mice was performed and the mice were sacrificed at different time points following exhaustive exercise. Our results confirmed that the full-length (130 kDa) and shorter-form (65 kDa) α-klotho were primarily expressed in the kidneys. Moreover, we found that, except for the kidneys and brain, other tissues primarily expressed the shorter-form α-klotho, including liver, which was in contrast to previous reports. Furthermore, the shorter-form α-klotho was decreased immediately following the acute exhaustive exercise and was then restored to the pre-exercise level or even higher levels in the next few days. Our results indicate that α-klotho may play a key role in the body exhaustion and recovery following exhaustive exercise.

Effect of combined aerobic and resistance exercise on serum Klotho secretion in healthy young men -a pilot study-

“…Increasing attention has been paid to the effects of KL on the prevention of cardiometabolic diseases. In this line, previous studies have investigated the impact of aerobic and/or resistance exercise on blood KL responses ( Matsubara et al, 2014 ; Santos-Dias et al, 2017 ; Baghaiee et al, 2018 ; Tan et al, 2018 ; Ramez et al, 2019 ; Rao et al, 2019 ). However, no study has examined the impact of combined resistance and aerobic exercise on blood KL concentrations.…”

“…In studies that focused on the impact of acute exercise, a significant increase in serum KL concentrations is observed in men and women ( Santos-Dias et al, 2017 ; Tan et al, 2018 ). By contrast, a single bout of exhaustive exercise in mice causes a significant reduction in serum KL levels ( Rao et al, 2019 ). We recently demonstrated that acute high-intensity resistance exercise increases serum KL concentrations in healthy young men ( Morishima and Ochi, 2021 ).…”

Impact of a single bout of resistance exercise on serum Klotho in healthy young men

“…Previous studies have investigated the impact of acute and long‐term aerobic exercise on blood Klotho responses (Baghaiee et al, 2018; Matsubara et al, 2014; Ramez et al, 2019; Rao et al, 2019; Santos‐Dias et al, 2017; Tan et al, 2018). However, no study examined whether a single bout of resistance exercise affects blood Klotho concentration.…”

“…Interestingly, serum Klotho concentrations were increased with a single bout of running exercise in men and women (Santos‐Dias et al, 2017; Tan et al, 2018). By contrast, a single bout of exhaustive exercise in mice causes a significant reduction in serum Klotho (Rao et al, 2019). Therefore, the blood Klotho concentration after a single bout of exercise is a controversial topic.…”

Potential Physiological and Cellular Mechanisms of Exercise That Decrease the Risk of Severe Complications and Mortality Following SARS-CoV-2 Infection

“…In rats, endurance training results in increased Klotho levels in the brain and kidneys, accompanied by an increased life span and attenuation of excessive ROS production [ 238 ]. In mice, the protein level of Klotho is acutely decreased in the skeletal muscle following one bout of exhaustive exercise, accompanied by a marked increase within 24 h compared to baseline [ 239 ]. The acute decrease in Klotho in the skeletal muscle may contribute to the increase in S-Klotho seen in studies of humans, which indicate a role of S-Klotho as a health-preserving myokine in the prevention of severe COVID-19.…”