α-L-fucosidase enhances capacitation-associated events in porcine spermatozoa

Romero-Aguirregomezcorta, Jon; Matás, Carmen; Coy, Pilar

doi:10.1016/j.tvjl.2014.11.006

Cited by 10 publications

(4 citation statements)

References 51 publications

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“…Previous studies showed the participation of fucosidase during fertilization and embryo development. Pre-incubation of oocytes with FUCA decreased the number of sperm bound to ZP [ 67 ], while the inhibition of FUCA activity reduced sperm penetration during bovine in vitro fertilization [ 68 , 69 ], and in turn, the oocyte was unable to pass the 2-pronuclear stage [ 69 ]. In the present study, higher levels of FUCA1 (Angus heifers) and FUCA2 (all animals) in the ipsilateral infundibulum linked to an upregulation of FUCA1 in the ipsilateral ampulla of LFC heifers, and suggests a positive regulation of fucosidase by ovulation; perhaps to guarantee fertilization and embryo development.…”

Section: Discussionmentioning

confidence: 99%

Can the antral follicular count modulate the gene expression of bovine oviducts in Aberdeen Angus and Nelore heifers?

et al. 2018

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The number of visible ovarian antral follicles (antral follicle count—AFC) is repeatable in bovine individuals, but highly variable between animals, and with differences between Bos taurus and Bos indicus breeds. Several studies have tried to determine the correlation between AFC and increased fertility in cattle. While the impacts of AFC on embryo production, hormonal levels, and pregnancy rates have been described, the molecular effects of AFC on bovine oviducts have not yet been investigated. Here, the aim was to investigate the impact of breeds, such as Aberdeen Angus and Nelore heifer with high or low AFC, on abundance of transcripts and protein related to oviductal transport, sperm reservoir formation, monospermy control, and gamete interaction in the oviducts. In summary, the ovulation side was the major factor that affected transcript abundance on bovine oviducts. However, a discreet effect among AFC and cattle breeds was also observed. Based on this, we concluded and reinforced here that differential microenvironments between ipsilateral and contralateral oviducts have a major effect on modulating the transcripts related to oviductal transport, sperm reservoir formation, monospermy control, and gamete interaction. However, we cannot exclude that there is minimal effect of AFC or breed on regulation of some genes (such as AGTR1, ACE1, FUCA1, and VEGFA) in bovine oviducts.

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Section: Discussionmentioning

confidence: 99%

Can the antral follicular count modulate the gene expression of bovine oviducts in Aberdeen Angus and Nelore heifers?

et al. 2018

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“…The stimulatory effect of E2 on oviduct fluid secretion (Ghersevich, Massa, & Zumoffen, ) results in the production of the greatest volume of fluid by the oviduct during estrus (Killian, ). Proteins like OVGP1, HSPA5 (also known as GRP78), FUCA1, and FUCA2 have been associated with spermatozoa and/or the cumulus‐oocyte complex (Boilard et al, ; Coy et al, ; Phopin, Nimlamool, Bartlett, & Bean, ; Phopin et al, ), acting on polyspermy control by hardening the zona pellucida before fertilization (Coy et al, ; Miller, Gong, Decker, & Shur, ; Mondéjar, Martínez‐Martínez, Avilés, & Coy, ), and modulating sperm‐ zona pellucida interactions, indicating an active role in the oviduct during the fertilization process (Coy et al, ; Marín‐Briggiler et al, ; Romero‐Aguirregomezcorta, Matás, & Coy, ; Venditti, Swann, & Bean, ). In this study, owing to the increase in local E2 levels in cows submitted to the FSH/eCG superstimulation protocol, we analyzed the transcript levels of genes involved in fertilization and gamete interactions.…”

Section: Discussionmentioning

confidence: 99%

Equine chorionic gonadotropin increases estradiol levels in the bovine oviduct and drives the transcription of genes related to fertilization in superstimulated cows

Fontes

Razza

Pupulim

et al. 2019

Molecular Reproduction Devel

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In the bovine oviduct, estradiol (E2) stimulates secretion and cell proliferation, whereas progesterone (P4) suppresses them. In this study, we have evaluated the effect of two superstimulatory protocols (follicle‐stimulating hormone [FSH] or FSH combined with equine chorionic gonadotropin [eCG]) on the oviductal levels of E2 and P4 and its outcome on oviductal cells. Compared with the control group (a single pre‐ovulatory follicle), we have observed that the cows submitted to FSH/eCG treatment showed a higher concentration of E2 in the oviduct tissue, together with a higher abundance of messenger RNA encoding steroid receptors (ESR1 and progesterone receptor), and genes linked to gamete interactions and regulation of polyspermy (oviduct‐specific glycoprotein 1, heat‐shock protein family A member 5, α‐l‐fucosidase 1 [FUCA1], and FUCA2) in the infundibulum and ampulla segments of the oviduct. However, we did not observe any modulation of gene expression in the isthmus segment. Even though the FSH protocol upregulated some of the genes analyzed, we may infer that the steady effect of FSH combined with eCG on oviduct regulation might benefit fertilization and may potentially increase pregnancy rates.

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“…Another compound, α-L-fucosidase, promotes sperm capacitation and prevents a premature acrosome reaction by increasing the sperm intracellular calcium concentration and tyrosine phosphorylation. It was also reported that α-L-fucosidase increased the ZP (zona pellucida) binding and penetration by the spermatozoa (Romero- Aguirregomezcorta et al 2015).…”

Section: Important Factors Involved In Sperm Capacitationmentioning

confidence: 93%

Polyspermic fertilisation of porcine oocytes in vitro - a current review

Chundekkad¹,

Stankiewicz

et al. 2019

Vet. Med. - Czech

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Polyspermic fertilisation has been a continual problem in porcine in vitro fertilisation. Over the years, researchers have tried to resolve this persistent problem, but have not been entirely successful in their endeavour. This can be attributed to several factors. This review discusses the role of sperm capacitation in the in vitro fertilisation and the most recent studies on the capacitation media. This includes the results obtained by testing the effect of various compounds on sperm capacitation. Additionally, the importance of the oviductal fluid on the porcine oocytes prior to fertilisation is also discussed. This is of significance, as the experiments show that oviductal fluid is crucial for the pre-fertilisation zona pellucida hardening. Also, the significance of using high quality oocytes for the in vitro fertilisation is highlighted. Lastly, the different and latest techniques to prevent or reduce polyspermic fertilisation have been reviewed.

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α-L-fucosidase enhances capacitation-associated events in porcine spermatozoa

Cited by 10 publications

References 51 publications

Can the antral follicular count modulate the gene expression of bovine oviducts in Aberdeen Angus and Nelore heifers?

Can the antral follicular count modulate the gene expression of bovine oviducts in Aberdeen Angus and Nelore heifers?

Equine chorionic gonadotropin increases estradiol levels in the bovine oviduct and drives the transcription of genes related to fertilization in superstimulated cows

Polyspermic fertilisation of porcine oocytes in vitro - a current review

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