α-Mangostin from Cratoxylum arborescens: An in vitro and in vivo toxicological evaluation

Ibrahim, Mohamed Yousif; Hashim, Najihah M.; Mohan, Syam; Abdulla, Mahmood Ameen; Abdelwahab, Siddig Ibrahim; Arbab, Ismail Adam; Yahayu, Maizatulakmal; Ali, Landa Zeenelabdin; Ishag, Omer Elhag

doi:10.1016/j.arabjc.2013.11.017

Cited by 43 publications

(38 citation statements)

References 40 publications

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“…On the 15 th day, the animals were overdosed with xylazine and ketamine. Histological, hematological and serum biochemical parameters were assessed following standard methods1718.…”

Section: Methodology and Materialsmentioning

confidence: 99%

In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

El-Ferjani

Ahmad

Dhiyaaldeen

et al. 2016

Sci Rep

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Co (II) complex (CMLA) was investigated to evaluate the rate of wound healing in rats. Animals were placed into four groups: gum acacia, Intrasite gel, 10 and 20 mg/ml of CMLA. Wounds were made on the dorsal neck area, then treated with Intrasite gel or CMLA; both of these treatments led to faster healing than with gum acacia. Histology of the wounds dressed with CMLA or Intrasite gel displayed a smaller scar width, required less time to heal and showed more collagen staining and fewer inflammatory cells in comparison to wounds dressed with the vehicle. Immunohistochemistry for Hsp70 and TGF-β showed greater staining intensity in the treated groups compared to the vehicle group. Bax staining was less intense in treated groups compared to the vehicle group, suggesting that CMLA and Intrasite gel provoked apoptosis, responsible for the development of granulation tissue into a scar. CD31 protein analysis showed that the treated groups enhanced angiogenesis and increased vascularization compared to the control group. Furthermore, a significant increase in the levels of GPx and SOD and a decrease in MDA were also observed in the treated groups. This results suggest that CMLA is a potentially promising agent for the wounds treatment.

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“…On the 15 th day, the animals were overdosed with xylazine and ketamine. Histological, hematological and serum biochemical parameters were assessed following standard methods1718.…”

Section: Methodology and Materialsmentioning

confidence: 99%

In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

El-Ferjani

Ahmad

Dhiyaaldeen

et al. 2016

Sci Rep

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“…Death cases were recorded over a duration of 14 consecutive days. All the rats were killed via an overdose of xylazine and ketamine anesthesia on the 15 th day and then histological evaluation and serum analysis were implemented following the standard techniques1718.…”

Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: Acute Toxicity and Gastroprotection Studies of a New Schiff Base Derived Manganese (II) Complex against HCl/Ethanol-Induced Gastric Ulcerations in Rats

Ibrahim

Hashim

Dhiyaaldeen

et al. 2016

Sci Rep

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Manganese is a crucial element for health. In this study, the gastroprotective efficacy of Mn (II) complex (MDLA) against acidified ethanol (HCl/Ethanol)-induced gastric ulceration in rats was evaluated. The animals were distributed into 5 groups. Groups 1 and 2 received carboxymethylcellulose (CMC), group 3 was pretreated with omeprazole, and groups 4 and 5 were given 10 and 20 mg/kg of MDLA, respectively. After one hour, CMC and HCl/Ethanol were given to groups 2–5 whilst the animals in group 1 were ingested with CMC. After sacrifice, gastric lesions were evaluated by wall mucus, gross appearance, histology, antioxidant enzymes and immunohistochemistry. Group 2 displayed severe gastric damage with a significant reduction in wall mucus. Conversely, gastric lesions were reduced in groups 3–5 by 85.72%, 56.51% and 65.93%, respectively. The rats in groups 3–5 showed up-regulation of heat shock protein 70 (Hsp70) with down-regulation of Bcl-2-associated protein x (Bax). Pretreatment with omeprazole or MDLA led to an increase in the uptake of Periodic Acid Schiff (PAS) stain in the glandular part of the gastric tissue, raised levels of prostaglandin E2 (PGE2) and superoxide dismutase (SOD), and a reduction in malondialdehyde (MDA) concentrations. These results suggested the gastroprotective action of Mn (II) complex.

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“…Similarly, previous study also revealed little cytotoxicity of α-M in normal cells and normal animals. 21 Here we demonstrate that the process involves caspase-3 and caspase-9 because these were significantly increased by the induction of α-M. Both of these caspases are known to either initiate or execute programmed cell death.…”

Section: Discussionmentioning

confidence: 62%

α‐Mangostin promotes apoptosis of human rheumatoid arthritis fibroblast‐like synoviocytes by reactive oxygen species‐dependent activation of ERK1/2 mitogen‐activated protein kinase

Sheng

Zhang

et al. 2019

J of Cellular Biochemistry

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α‐Mangostin (α‐M) is a commonly used traditional medicine with various biological and pharmacological activities. Our study aimed to explore the effects and mechanism of α‐M in regulating apoptosis of rheumatoid arthritis fibroblast‐like synoviocytes (RA‐FLS). α‐M of 10 to 100 μM was used to treat RA‐FLS for 24 hours, followed by measuring cell viability and apoptosis. The involvement of reactive oxygen species (ROS) and mitogen‐activated protein kinases was detected. Treatment of α‐M promoted apoptosis and reduced viability of RA‐FLS in a dose‐dependent manner. The mitochondrial membrane potential in RA‐FLS was remarkably reduced by α‐M treatment, accompanied by the cytochrome c accumulation in the cytosol and increased activities of caspase‐3 and caspase‐9. Moreover, we found that α‐M treatment promoted ROS production and extracellular signal‐regulated kinase 1/2 (ERK1/2) phosphorylation. The proapoptotic activity of α‐M in RA‐FLS was markedly reversed by the co‐induction with the ERK1/2 inhibitor LY3214996 or ROS scavenger N‐acetyl‐l‐cysteine. In conclusion, our studies found that α‐M had remarkable proapoptotic activities in RA‐FLS, which is regulated by the induction of ROS accumulation and ERK1/2 phosphorylation. α‐M may thus have potential therapeutic effects for rheumatoid arthritis.

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α-Mangostin from Cratoxylum arborescens: An in vitro and in vivo toxicological evaluation

Cited by 43 publications

References 40 publications

In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

RETRACTED ARTICLE: Acute Toxicity and Gastroprotection Studies of a New Schiff Base Derived Manganese (II) Complex against HCl/Ethanol-Induced Gastric Ulcerations in Rats

α‐Mangostin promotes apoptosis of human rheumatoid arthritis fibroblast‐like synoviocytes by reactive oxygen species‐dependent activation of ERK1/2 mitogen‐activated protein kinase

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