α-methylacyl-CoA racemase (P504S)/34βE12/p63 triple cocktail stain in prostatic adenocarcinoma after hormonal therapy

Sung, Ming Tse; Jiang, Zhong; Montironi, Rodolfo; MacLennan, Gregory T.; Mazzucchelli, Roberta; Liu, Cheng

doi:10.1016/j.humpath.2006.08.016

Cited by 39 publications

(18 citation statements)

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“…The occasional absence of basal cells in partial atrophy and adenosis along with outpouching of highgrade prostatic intraepithelial neoplasia (HPIN) may yield false-negative results with basal cell markers (high-molecular weight keratin and p63) [6,7]. AMACR reactivity is absent in approximately 25% of prostate cancers [7,8] and 36% of HPIN [9]. Other limitations of AMACR include cytoplasmic staining, which can be ambiguous, and the lack of increased staining intensity with increasing Gleason pattern [5,6].…”

Section: Introductionmentioning

confidence: 99%

Histone H1.5, a novel prostatic cancer marker: an immunohistochemical study

et al. 2014

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Section: Introductionmentioning

confidence: 99%

Histone H1.5, a novel prostatic cancer marker: an immunohistochemical study

et al. 2014

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“…23 immune reaction. New multiplexing techniques that combine tyramide-signal amplification and multispectral imaging are being developed and permit complex analyses of the spatial interactions between tumors and many classes of immune cells, eg, see Stack et al 3 Prostate cancer diagnostics could also benefit from clinically directed multiplexing, as core biopsies could be routinely examined using antibodies defining basal and luminal cells and cells with upregulated racemase, 37,38 as well as antibody panels that might survey protein products of the Genomic Health prostate panel of 17 genes that has been developed to stratify patients into progression-risk categories. 39 It is difficult to get an accurate evaluation of the falsepositive and false-negative rates of prostate core biopsies, but it is clear that the use of combined 3-antibody IHC has become much more frequent and has probably decreased these rates.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemistry and mass spectrometry for highly multiplexed cellular molecular imaging

Levenson

Borowsky

Angelo

2015

Laboratory Investigation

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The role of immunohistochemistry (IHC) in the management of cancer has expanded to provide improved diagnostic classification, as well as guidance on disease prognosis, therapy, and relapse. These new tasks require evaluation of an increasing number of protein targets; however, conventional multiplexing, usually achieved using serial tissue sections stained for a single analyte per slide, can exhaust small biopsy specimens, complicate slide-to-slide protein expression correlation, and leave insufficient material for additional molecular assays. A new approach, mass spectrometry immunohistochemistry (MSIHC), compatible with high levels of target multiplexing and suitable for use on formalin-fixed, paraffin-embedded samples can circumvent many of these issues. The strategy employs antibodies that are labeled with elemental mass tags, such as isotopically pure lanthanides not typically found in biological specimens, rather than with typical fluorophores or chromogens. The metal-labeled antibodies are then detected in tissue using lasers or ion beams to liberate the tags for subsequent mass spectrometry detection. Within a given multiplexed IHC panel, the metal labels are selected so that their respective masses do not overlap. More than 30 antibodies have been imaged simultaneously, and up to 100 antibodies could potentially be detected at once if the full available mass spectrum is deployed. MSIHC has a number of advantages over conventional IHC techniques. Background due to autofluorescence is absent and the dynamic range is 10 5 , exceeding immunofluorescence and chromogenic IHC by 100-fold and 1000-fold, respectively. Detection of labeled primary antibodies improves assay linearity over both chromogenic and fluorescent IHC. Multiplexed mass-tagged antibodies incubated simultaneously with tissue do not appear to cross-interfere, and because the mass tags do not degrade, samples are stable indefinitely. The imaging resolution of multiplexed ion-beam imaging can be better than light microscopy. With appropriate instrumentation, MSIHC has the potential to transform research and clinical pathology practice.

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“…When we reviewed prostate specimens obtained through fine needle biopsy, we noted especial difficulties in interpreting minute foci of atypical acini (atypical hyperplasia (AHP) vs atypical small acinar proliferation (ASAP) vs welldifferentiated prostate adenocarcinoma (PCA) (Montironi EU 2006) [43], and of atypia in neoplastic prostate glands after hormonal or radiotherapy (Sung HP 2007) [44]. The main causes of discordance in the prostate specimens obtained through TURP were problems in assessing the histological grade of intraepithelial prostatic neoplasia (PIN) (low-grade vs high-grade PIN; high-grade PIN vs microinvasive PCA), and in assessing the Gleason score histologic grading (Montironi NCPU 2007) [45] (Montironi JCP 2007) [46].…”

Section: Discussionmentioning

confidence: 99%

Diagnostic 'Errors' in a Single Urogenital Pathology Unit

Cardillo¹

2008

TOPATJ

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Abstract:To understand better the factors that contribute to diagnostic errors in urogenital pathology units, as part of our ongoing research into urogenital cancers, from a consecutive series of 6304 histologic reports for the years 2003 to 2006 we selected for review those containing more than one evident error. We reviewed 1746 urogenital histopathologic diagnoses, 1459 (83.56%) referred to biopsies and 287 (16.43%) to surgical specimens. Of the 1746 reports reviewed, 1643 diagnoses (94.10%) were in agreement and 103 (5.89%) in disagreement with the reviewer's rating. Among these 103 diagnoses, 77 (70.75%) had justifiable and 27 (26.21%) unjustifiable discordances. Of these 27 discordances, 23 were unjustifiable discordances containing errors with consequences that affected patient management. The poor diagnostic efficiency found in a single anatomopathological unit raises concern and suggests considerable room for improvement. Efficiency could be substantially improved if pathological services strictly applied the existing international measures for quality control.

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α-methylacyl-CoA racemase (P504S)/34βE12/p63 triple cocktail stain in prostatic adenocarcinoma after hormonal therapy

Cited by 39 publications

References 50 publications

Histone H1.5, a novel prostatic cancer marker: an immunohistochemical study

Histone H1.5, a novel prostatic cancer marker: an immunohistochemical study

Immunohistochemistry and mass spectrometry for highly multiplexed cellular molecular imaging

Diagnostic 'Errors' in a Single Urogenital Pathology Unit

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