α‐Synuclein concentration increases over time in plasma supernatant of single donor platelets

Stefaniuk, Catherine M.; Hong, Hong; Maitta, Robert W.

doi:10.1111/ejh.13152

Cited by 8 publications

(8 citation statements)

References 29 publications

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“…The plasma α-synuclein level was significantly higher than the α-synuclein level in serum. Higher plasma α-synuclein level may be attributed to cell lysis of free erythrocyte and platelets (38), and to more α-synucleincontaining exosomes from free erythrocytes (33), whereas serum might contain fewer exosomes because of erythrocytes that are trapped in the fibrin complex. Furthermore, proteases such as plasmin in platelet activation cleaved free α-synuclein from cell lysis in serum (39) during clot formation; by contrast, materials in exosome may be protected (40).…”

Section: Discussionmentioning

confidence: 99%

Plasma and Serum Alpha-Synuclein as a Biomarker of Diagnosis in Patients With Parkinson's Disease

Chang

Yang²,

Yang³

et al. 2020

Front. Neurol.

107

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Background: Parkinson's disease (PD) is the second most common neurodegenerative disease, and α-synuclein plays a critical role in the pathogenesis of PD. Studies have revealed controversial results regarding the correlation between motor severity and α-synuclein levels in peripheral blood from patients with PD. Objective: We examined α-synuclein levels in plasma or serum in patients with PD and investigated the relationship between plasma or serum α-synuclein level and motor symptom severity. Methods: We recruited 88 participants (48 patients with PD and 40 healthy controls). Clinical information was collected, and venous blood was drawn from each participant to be processed to obtain plasma or serum. The plasma or serum α-synuclein level was detected using monoclonal antibodies with magnetic nanoparticles, and was measured through immunomagnetic reduction. Plasma or serum α-synuclein levels were quantitatively detected. Results: In patients with PD, the means of plasma and serum α-synuclein level were 3.60 ± 2.53 and 0.03 ± 0.04 pg/mL, respectively. The areas under the receiver operating characteristic curve of plasma and serum α-synuclein for distinguishing patients with PD from healthy controls were 0.992 and 0.917, respectively. The serum α-synuclein level also showed a significant correlation with patients in H-Y stages 1-3 (r = 0.40, p = 0.025), implying that the serum α-synuclein level may be a potential marker of motor symptom severity in patients with early PD. Conclusions: Our data suggest that the α-synuclein level in serum or plasma can differentiate between healthy controls and patients with PD. Serum α-synuclein levels moderately correlate with motor severity in patients with early PD.

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Section: Discussionmentioning

confidence: 99%

Plasma and Serum Alpha-Synuclein as a Biomarker of Diagnosis in Patients With Parkinson's Disease

Chang

Yang²,

Yang³

et al. 2020

Front. Neurol.

107

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“…Of interest, α-syn concentration increases in a time-dependent manner in plasma of single donor platelets during storage. This increase could result from platelets releasing their contents due to senescence or because of storage-dependent changes to platelets [31].…”

Section: Main Textmentioning

confidence: 99%

“…Notably, increased concentrations of plasma α-syn are observed in PD patients secondary to neuronal degeneration that increases its concentration in CSF with subsequent efflux into plasma [29]. Interestingly, release of α-syn into plasma can also occur in a time-dependent manner secondary to aging of platelets as recently described in single donor platelet units during storage making it also a potential biomarker for studying biochemical changes during platelet storage [31].…”

Section: Introductionmentioning

confidence: 99%

Alpha synuclein in hematopoiesis and immunity

Pei

Maitta

2019

Heliyon

Self Cite

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A B S T R A C TParkinson's disease (PD) is the second most common neurodegenerative condition and intracellular deposition of Lewy bodies in the substantia nigra (SN), which can cause dopaminergic neuronal death, is the hallmark of this syndrome. α-synuclein (syn) is a small protein expressed mainly in neurons but can also be found in a number of tissues. It can be present as a soluble monomer under normal physiological conditions, but can be toxic in its oligomeric or fibrillary forms. Most of the available literature has focused on the effects of α-syn pathology in the mechanisms leading to PD. However, the normal functions of α-syn still remain to be fully elucidated. Notably, α-syn in the hematopoietic system seems to mediate important functions as indicated by anemia and incomplete cell maturation when this protein is absent. This review will summarize basic genetic and structural findings, and critical information that suggests an essential role of α-syn in the development and activation of the hematopoietic system and immunity.

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“…α-synuclein expression has also been reported to be a specific marker of neoplastic changes in the megakryocytic lineage [7]. Platelets have been shown to have the highest concentration of α-synuclein per milligram of cellular protein in peripheral blood [8], and time-dependent α-synuclein concentration increases in plasma supernatant of stored platelets may be indicative of changes that platelets undergo while in storage [9]. Importantly, αsynuclein in platelets has been shown to inhibit α-granule release in a calcium dependent manner [10].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural changes in peripheral blood leukocytes in α-synuclein knockout mice

Tashkandi

Shameli

Maitta

2018

Blood Cells, Molecules, and Diseases

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Effects of α-synuclein deficiency on cellular blood components have not been extensively investigated. This study evaluated ultrastructural changes of leukocytes in α-synuclein knockout (KO) mice using electron microscopy (EM). The following ultrastructural characteristics were quantified in leukocytes: mitochondria, primary granules, specific granules (SG), Golgi apparatus (GA), inclusions, rough-endoplasmic reticulum (RER), smooth-endoplasmic reticulum (SER), and cellular projections (CP). EM showed increased numbers or amounts of SG, inclusions, and SER in KO group (5.3 ± 4.5 in WT vs. 14.1 ± 10.3 in KO, p = 0.02; 0.4 ± 0.9 in WT vs. 3.2 ± 2.8 in KO, p = 0.007; and 7.7 ± 6.7 in WT vs. 17.7 ± 12.2 in KO, p = 0.03, respectively). Although CP number was not significantly different between the two groups (13.4 ± 5.3 in WT vs. 16.3 ± 7.5 in KO, p = 0.32), their size and shapes were altered in KO mice. Notably, findings occurred in the setting of significant lymphopenia. α-Synuclein deficiency leads to changes in size and shape of secretory particles and increases in SER, SG, and inclusions, indicating a potential role for α-synuclein in vesicular trafficking in leukocytes. Further studies are needed to elucidate functions mediated by α-synuclein.

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α‐Synuclein concentration increases over time in plasma supernatant of single donor platelets

Cited by 8 publications

References 29 publications

Plasma and Serum Alpha-Synuclein as a Biomarker of Diagnosis in Patients With Parkinson's Disease

Plasma and Serum Alpha-Synuclein as a Biomarker of Diagnosis in Patients With Parkinson's Disease

Alpha synuclein in hematopoiesis and immunity

Ultrastructural changes in peripheral blood leukocytes in α-synuclein knockout mice

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