α1,3 Glucans Are Dispensable in Aspergillus fumigatus

Henry, Christine; Latgé, Jean Paul; Beauvais, Anne

doi:10.1128/ec.05270-11

Cited by 86 publications

(95 citation statements)

References 29 publications

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“…Even though the cell wall of the triple ags mutant is devoid of a (1,3) glucan, fungal growth and conidiation is not affected in vitro [25]. However, this mutant was avirulent in neutropenic mice due to a block in conidial germination [20 ].…”

Section: A(13) Glucan: a Polysaccharide With Dual Functionsmentioning

confidence: 99%

Functional duality of the cell wall

Latgé¹,

Beauvais²

2014

Current Opinion in Microbiology

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121

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Section: A(13) Glucan: a Polysaccharide With Dual Functionsmentioning

confidence: 99%

Functional duality of the cell wall

Latgé¹,

Beauvais²

2014

Current Opinion in Microbiology

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121

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“…Fission yeast contains four additional Ags1 homologues (Mok11 to Mok14), which are detected only during sporulation (81). Ags1 orthologues are not found in budding yeasts but are widely extended in filamentous, dimorphic, and pathogenic fungi (82,83).…”

Section: ␣(13)-d-glucan Synthase: Ags1/mok1mentioning

confidence: 99%

The Cell Biology of Fission Yeast Septation

Cortés

Ramos

Osumi

et al. 2016

Microbiol Mol Biol Rev

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SUMMARYIn animal cells, cytokinesis requires the formation of a cleavage furrow that divides the cell into two daughter cells. Furrow formation is achieved by constriction of an actomyosin ring that invaginates the plasma membrane. However, fungal cells contain a rigid extracellular cell wall surrounding the plasma membrane; thus, fungal cytokinesis also requires the formation of a special septum wall structure between the dividing cells. The septum biosynthesis must be strictly coordinated with the deposition of new plasma membrane material and actomyosin ring closure and must occur in such a way that no breach in the cell wall occurs at any time. Because of the high turgor pressure in the fungal cell, even a minor local defect might lead to cell lysis and death. Here we review our knowledge of the septum structure in the fission yeastSchizosaccharomyces pombeand of the recent advances in our understanding of the relationship between septum biosynthesis and actomyosin ring constriction and how the two collaborate to build a cross-walled septum able to support the high turgor pressure of the cell. In addition, we discuss the importance of the septum biosynthesis for the steady ingression of the cleavage furrow.

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“…Among the Aspergillus species, the functions of α-1,3-glucan synthase genes have been studied in A. fumigatus and A. niger [11]–[14]. Aspergillus fumigatus contains three AGS genes, ags 1 to ags 3.…”

Section: Introductionmentioning

confidence: 99%

“…The expression of agsA (an orthologue of A. fumigatus ags3 ) and agsE (an orthologue of A. fumigatus ags1 and A. nidulans agsB ) was induced in the presence of cell wall stress–inducing compounds such as calcofluor white (CFW), sodium dodecyl sulfate, and caspofungin [13]. More recently, a triple-mutant strain of A. fumigatus lacking the three α-1,3-glucan synthase genes ( ags1 , ags2 , and ags3 ) was generated, and growth of the triple mutant in plate culture was similar to that of the parental strain [14]. The triple mutant showed slightly decreased conidiogenesis, as did the single ags1 and ags2 mutants [11], [14], and the lack of cell wall α-1,3-glucan led to an increase in β-1,3-glucan and chitin levels in mycelia of the triple mutant [14].…”

Section: Introductionmentioning

confidence: 99%

“…More recently, a triple-mutant strain of A. fumigatus lacking the three α-1,3-glucan synthase genes ( ags1 , ags2 , and ags3 ) was generated, and growth of the triple mutant in plate culture was similar to that of the parental strain [14]. The triple mutant showed slightly decreased conidiogenesis, as did the single ags1 and ags2 mutants [11], [14], and the lack of cell wall α-1,3-glucan led to an increase in β-1,3-glucan and chitin levels in mycelia of the triple mutant [14]. Despite these studies of the functions of α-1,3-glucan synthase genes, the biological roles of α-1,3-glucan are only partly understood.…”

Section: Introductionmentioning

confidence: 99%

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Functional Analysis of the α-1,3-Glucan Synthase Genes agsA and agsB in Aspergillus nidulans: AgsB Is the Major α-1,3-Glucan Synthase in This Fungus

et al. 2013

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Although α-1,3-glucan is one of the major cell wall polysaccharides in filamentous fungi, the physiological roles of α-1,3-glucan remain unclear. The model fungus Aspergillus nidulans possesses two α-1,3-glucan synthase (AGS) genes, agsA and agsB. For functional analysis of these genes, we constructed several mutant strains in A. nidulans: agsA disruption, agsB disruption, and double-disruption strains. We also constructed several CagsB strains in which agsB expression was controlled by the inducible alcA promoter, with or without the agsA-disrupting mutation. The agsA disruption strains did not show markedly different phenotypes from those of the wild-type strain. The agsB disruption strains formed dispersed hyphal cells under liquid culture conditions, regardless of the agsA genetic background. Dispersed hyphal cells were also observed in liquid culture of the CagsB strains when agsB expression was repressed, whereas these strains grew normally in plate culture even under the agsB-repressed conditions. Fractionation of the cell wall based on the alkali solubility of its components, quantification of sugars, and 13C-NMR spectroscopic analysis revealed that α-1,3-glucan was the main component of the alkali-soluble fraction in the wild-type and agsA disruption strains, but almost no α-1,3-glucan was found in the alkali-soluble fraction derived from either the agsB disruption strain or the CagsB strain under the agsB-repressed conditions, regardless of the agsA genetic background. Taken together, our data demonstrate that the two AGS genes are dispensable in A. nidulans, but that AgsB is required for normal growth characteristics under liquid culture conditions and is the major AGS in this species.

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α1,3 Glucans Are Dispensable in Aspergillus fumigatus

Cited by 86 publications

References 29 publications

Functional duality of the cell wall

Functional duality of the cell wall

The Cell Biology of Fission Yeast Septation

Functional Analysis of the α-1,3-Glucan Synthase Genes agsA and agsB in Aspergillus nidulans: AgsB Is the Major α-1,3-Glucan Synthase in This Fungus

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