β-1,3-glucanases expression of Beauveria bassiana in culture with extract of the phytopathogenic Peronospora variabilis and Fusarium oxysporum

Montoya, W. Jhoel; Nolasco, Oscar; Acuña, Rosalyn; Gutiérrez, Ana

doi:10.17268/sci.agropecu.2016.03.13

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…These are synthesized at the tip of the hyphae during cell growth [59,76], but in Fol it is found as an internal layer [61]. The ability of B. bassiana to use F. oxysporum as a carbon source in the production of β-1,3-glucanases has been observed in the in vitro test carried out by [77], where expression of 60% more Exo-β-1,3-glucanase genes was reported as of 7 dai. The results found by [78] show that the β-1,3-glucanases produced by Bacillus sp., show a peak activity in the middle of experimentation (15.61 U mL −1 •min −1 ), which subsequently decrease.…”

Section: Enzymatic Assay Of Solanum Lycopersicum Plants Inoculated Wi...mentioning

confidence: 99%

Antifungal Potential of Beauveria bassiana on Solanum lycopersicum L. Infected with Fusarium oxysporum f. sp. lycopersici

L髉ez-L髉ez¹,

Ruíz-Lau²,

Meza-Gordillo³

et al. 2023

Phyton

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The objective of this work was to evaluate the effect of Beauveria bassiana (Bb 1205) on controlling Fusarium oxysporum f. sp. lycopersici (Fol 17108) in tomato plants in greenhouse conditions. Inoculation of Bb 1205 was the most promising among the agronomic variables and expression of the activity of the enzymes β-1,3-glucanases and chitinases. Inoculation of Bb 1205 occurred at a concentration of 1 × 10 8 conidia•mL −1 , which was administered onto the leaves, directly into the soil and via injection. Infection with Fol 17108 occurred with 1 × 10 6 spores•mL −1 , which were added directly to the soil. Spectrophotometry was used for measuring agronomic parameters, namely activity of chitinases and β-1,3-glucanases in foliage and roots. When Bb 1205 was added to the soil, the chlorophyll index and aerial part length showed significant differences. In addition, it was determined that root length, fresh weight of foliage, flower, and fruit count increased 82 days after inoculation (dai). Chitinase activity induced by Bb 1205 in leaves and roots of tomato plants infected with Fol 17108 was observed when injected into the stem at 32 dai (41.8 and 11.6-fold, respectively). Inoculation on the foliage showed a 10-fold increase of β-1,3-glucanases in the roots after 82 dpi. As for leaves, a 3.8-fold increase was found when the stem was inoculated. In the different in vivo applications, Bb 1205 activated its defenses by expressing the chitinase enzymes and β-1,3-glucanase, thus reducing the damage caused by Fol 17108, demonstrating increase plant growth thereafter.

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Section: Enzymatic Assay Of Solanum Lycopersicum Plants Inoculated Wi...mentioning

confidence: 99%

Antifungal Potential of Beauveria bassiana on Solanum lycopersicum L. Infected with Fusarium oxysporum f. sp. lycopersici

L髉ez-L髉ez¹,

Ruíz-Lau²,

Meza-Gordillo³

et al. 2023

Phyton

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“…For example, Bacillus subtilis produced beta-1,3-glucanase and exerted antifungal activity [19]. Entomopathogenic fungi such as the fungus Beauveria bassiana Vuill are also valuable antagonists of pathogenic fungi, as they produce a β-1,3-glucanase useful for biotechnology [20]. Anaerobic bacteria such as Thermotoga maritima MSB8, whose optimum temperature is 80 • C, also show interest in producing β-1,3-glucanase active at elevated temperatures [21].…”

Section: Introductionmentioning

confidence: 99%

A First Expression, Purification and Characterization of Endo-β-1,3-Glucanase from Penicillium expansum

Wang,

Huai,

Tan

et al. 2023

JoF

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β-1,3-glucanase plays an important role in the biodegradation, reconstruction, and development of β-1,3-glucan. An endo-β-1,3-glucanase which was encoded by PeBgl1 was expressed, purified and characterized from Penicillium expansum for the first time. The PeBgl1 gene was amplified and transformed into the competent cells of E. coli Rosetta strain with the help of the pET-30a cloning vector. The recombinant protein PeBgl1 was expressed successfully at the induction conditions of 0.8 mmol/L IPTG at 16 °C for 16 h and then was purified by nickel ion affinity chromatography. The optimum reaction temperature of PeBgl1 was 55 °C and it had maximal activity at pH 6.0 according to the enzymatic analysis. Na2HPO4-NaH2PO4 buffer (pH 6.0) and NaCl have inhibitory and enhancing effects on the enzyme activities, respectively. SDS, TritonX-100 and some metal ions (Mg2+, Ca2+, Ba2+, Cu2+, and Zn2+) have an inhibitory effect on the enzyme activity. The results showed that PeBgl1 protein has good enzyme activity at 50–60 °C and at pH 5.0–9.0, and it is not a metal dependent enzyme, which makes it robust for storage and transportation, ultimately holding great promise in green biotechnology and biorefining.

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Mechanisms of action of the yeast Debaryomyces nepalensis for control of the pathogen Colletotrichum gloeosporioides in mango fruit

Zhou

Zeng

et al. 2018

Biological Control

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β-1,3-glucanases expression of Beauveria bassiana in culture with extract of the phytopathogenic Peronospora variabilis and Fusarium oxysporum

Cited by 3 publications

References 14 publications

Antifungal Potential of Beauveria bassiana on Solanum lycopersicum L. Infected with Fusarium oxysporum f. sp. lycopersici

Antifungal Potential of Beauveria bassiana on Solanum lycopersicum L. Infected with Fusarium oxysporum f. sp. lycopersici

A First Expression, Purification and Characterization of Endo-β-1,3-Glucanase from Penicillium expansum

Mechanisms of action of the yeast Debaryomyces nepalensis for control of the pathogen Colletotrichum gloeosporioides in mango fruit

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