β-adrenergic receptor subtype transcripts in porcine adipose tissue

Rl, McNeel; Mersmann, H. J.

doi:10.2527/1995.7371962x

Cited by 19 publications

(11 citation statements)

References 27 publications

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“…A possible explanation may be differences in the sensitivity to the f}-adrenergic agonist, perhaps related to receptor sensitivity or number such as that described recently for pig fat depots by McNeel and Mersmann (1995). These conclusions were evident on the basis of actual data recorded for animals or calculated in comparison with the initial slaughter group.…”

Section: Discussionmentioning

confidence: 75%

Effect of the β-adrenergic agonist cimaterol and dietary protein level on fat young sheep given diets containing submaintenance levels of dietary energy

1997

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Thirty Suffolk cross wether sheep aged about 12 months and weighing 57 kg on average were used. They had been given previously a high energy diet to produce a fat body condition and average condition score of 3-7. Animals 'were allocated to an initial slaughter group (IS) or to four dietary treatment groups (UL, UH, CL and CH) based on sugar-beet feed (L) or white-fish meal (H) supplemented with chopped straw and pelleted, without (UL and UH) or with (CL and CH) the (5-adrenergic agonist cimaterol, to provide an estimated 20 g (L) or 130 g (H) rumen undegradable protein per kg dry matter (DM). The sheep were offered 0-3 kg of the appropriate pelleted diet in addition to 200 to 400 g of chopped barley straw to provide proportionately 0-7 of the estimated metabolizable energy requirement for maintenance and, where appropriate, 2-5 mg cimaterol per day. Comparisons were made for the main effects of dietary protein intake and cimaterol and interactions between dietary protein and cimaterol. The animals on dietary treatments were slaughtered after 49 days. The animals exhibited variable reductions in live weight and weights of carcass and non-carcass components in comparison with the IS group and in response to restricted dietary energy intake. The only effect due to the elevated dietary protein provision was a smaller loss in live weight compared with the low protein diet. In contrast, compared with responses recorded in its absence, treatment with cimaterol resulted in significantly greater values for weights of cold carcass, cross-sectional area o/m. longissimus dorsi and weights of DM, crude protein and ash in the carcass with significantly smaller weights for raw fleece. In comparison with the IS group, significantly smaller changes due to cimaterol were recorded for the weights of carcass crude protein and ash. Cimaterol treatment significantly increased the weight loss of fat in the carcass and in the perirenal and retroperitoneal, but not omental, depots as components of fleece-free non-carcass fat which was also significantly reduced in total. Interactions suggested that losses in carcass weight and DM in the presence of cimaterol were less and gains in carcass protein and ash greater, on the high than on the low protein diet. The results suggest that under conditions of energy undernutrition cimaterol may induce smaller losses or larger gains in carcass but not non-carcass, crude protein at the expense of fat depletion in carcass and non-carcass fat depots.

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Section: Discussionmentioning

confidence: 75%

Effect of the β-adrenergic agonist cimaterol and dietary protein level on fat young sheep given diets containing submaintenance levels of dietary energy

1997

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“…The 2.0 kb ADRB3 transcript in the bovine brown adipose tissue (PietriRouxel et al, 1995), and two transcripts of 2.2 and 1.9 kb in porcine subcutaneous fat (McNeel and Mersmann, 1995) were detected by Northern blotting. The mRNA expressed in the adipose tissue (Sumner and McNamara, 2007), mammary gland (Inderwies et al, 2003), gastrointestinal tracts including the cecum, ileum, colon and abomasum (Meylan et al, 2004;Kobel et al, 2006) and in the liver (Carron et al, 2005) of cattle through real-time polymerase chain reaction (RT-PCR) or ligand binding assay.…”

Section: Discussionmentioning

confidence: 99%

Distribution and quantification of β-3 adrenergic receptor in tissues of sheep

Liu

et al. 2011

Animal

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The b-3 adrenergic receptor (ADRB3) is a G-protein coupled receptor involved in regulating lipolysis, as part of homeostatic regulation. In this study, South African Mutton Merino and Shanxi Dam Line were used to study the distribution and quantification of ADRB3 in adipose (subcutaneous, omental, retroperitoneal, mesenteric and perirenal fat) and non-adipose (heart, liver, spleen, lung and kidney) tissues of sheep. The protein was determined by immunohistochemical technique and by mRNA abundance via real-time polymerase chain reaction. ADRB3 was detected in all studied tissues with abundance in adipose tissues higher than in non-adipose tissues ( P , 0.001). For adipose tissues, greater expression was found in deep deposits such as great omental and retroperitoneal fat than in subcutaneous fat ( P , 0.05). Significant differences ( P , 0.05) both for mRNA and for protein expression also existed between the two sheep flocks. These findings are consistent with the known function of ADRB3 in mediating lipolysis and homeostasis in adipose tissues.Keywords: b-3 adrenergic receptor, sheep, tissues, mRNA expression, immunohistochemistry ImplicationsIn this study, the b-3 adrenergic receptor (ADRB3) expression in various tissues of sheep was detected by immunohistochemical technique and by mRNA abundance via real-time PCR. Despite its low expression, the presence of ADRB3 in the spleen and kidney was found in livestock for the first time. Generally, ADRB3 expressed higher in adipose than in non-adipose tissues, and for the former, it was higher in deep fat deposits than in subcutaneous fat. The distributions are consistent with the known functions of ADRB3 in mediating lipolysis and homeostasis in adipose tissues, and relaxation of vascular smooth muscle in the cardiovascular system. The relation between low expression and its function in some viscera, for example, lung, spleen and kidney of sheep deserves further study.Introduction b-Adrenergic receptors (ADRBs) are G-protein coupled receptors that mediate effects of the endogenous catecholamines adrenaline and noradrenaline. Three subtypes of ADRBs have been identified, named ADRB1, ADRB2 and ADRB3, respectively. These receptors are present in most mammalian tissues, but the distribution of each subtype varies among tissues in a given species. Among the various physiological roles of ADRBs, the ADRB3 subtype mediates lipolysis and stimulates thermogenesis in adipose tissues (Cannon and Nedergaard, 2004) by activating uncoupling protein in mitochondria.In early studies, ADRB3 was identified mainly on the surface of white and brown adipocytes as reviewed by Strosberg (1997). In recent years, expression of the protein was also found in the human heart (Rozec and Gauthier , (Rodriguez et al., 1995).It has been confirmed that the mRNA of the ADRB3 gene is expressed in various tissues of domestic animals. The 2.0 kb ADRB3 transcript in the bovine brown adipose tissue (PietriRouxel et al., 1995), and two transcripts of 2.2 and 1.9 kb in porcine subcutaneous fat (McNeel and ...

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“…For example, in pigs skeletal muscle has more BAR2 than BAR1 whilst in adipose tissue it is the reverse (Liang & Mills, 2002). BAR3 are thought to be present on porcine adipocytes (McNeel & Mersmann, 1995).…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

The impact of growth promoters on muscle growth and the potential consequences for meat quality

Parr

Mareko²,

Ryan

et al. 2016

Meat Science

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To meet the demands of increased global meat consumption, animal production systems will have to become more efficient, or at least maintain the current efficiency utilizing feed ingredients that are not also used for human consumption. Use of growth promoters is a potential option for increasing production animal feed efficiency and increased muscle growth. The objective of this manuscript is to describe the mechanisms by which the growth promoters, beta-adrenergic agonists and growth hormone, mediate their effects, with specific consideration of the aspects which have implications for meat quality.

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β-adrenergic receptor subtype transcripts in porcine adipose tissue

Cited by 19 publications

References 27 publications

Effect of the β-adrenergic agonist cimaterol and dietary protein level on fat young sheep given diets containing submaintenance levels of dietary energy

Effect of the β-adrenergic agonist cimaterol and dietary protein level on fat young sheep given diets containing submaintenance levels of dietary energy

Distribution and quantification of β-3 adrenergic receptor in tissues of sheep

The impact of growth promoters on muscle growth and the potential consequences for meat quality

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