β-Adrenoreceptors in the Trout (Oncorhynchus mykiss) Heart: Characterization, Quantification, and Effects of Repeated Catecholamine Exposure

Gamperl, A. Kurt; Wilkinson, Michael; Boutilier, Robert G.

doi:10.1006/gcen.1994.1123

Cited by 74 publications

(53 citation statements)

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“…This conclusion contrasts with the previously held belief that the flounder heart has equal affinity for A and NA (Ask, 1983), but agrees with Cobb and Santer (Cobb and Santer, 1973) who showed that the isolated heart of the flounder P. platessa is more sensitive to A than to NA, and with Gamperl et al (Gamperl et al, 1994a) who characterized the ventricular β-adrenoreceptors of the rainbow trout heart as β 2 . Nonetheless, the high K d measured for these receptors (1.02 nmol l -1 CGP) is difficult to reconcile with previous studies on fish which report K d values in the range of ~0.1-0.4 nmol l -1 (Gamperl et al, 1994a;Olsson et al, 2000). One possible explanation for the high K d value measured for β-adrenoreceptors of the flounder heart is that they are functionally uncoupled from adenylate cyclase (Hausdorff et al, 1990).…”

Section: Catecholamine Dose-response Curves and Cardiac β-Adrenorecepsupporting

confidence: 73%

“…33%. This diminished adrenergic sensitivity in flounder, as compared with rainbow trout, hearts is clearly not due to a reduced number of cardiac P. C. Mendonça and A. K. Gamperl β-adrenoreceptors, as β-adrenoreceptor density in the flounder ventricle (B max , 252.8 fmol mg -1 protein) is the highest ever reported for a teleost species, and six-to 12-fold higher than reported for the rainbow trout (23-40 fmol mg -1 protein) (Gamperl et al, 1994a;Olsson et al, 2000). A more likely explanation is that the injected concentrations were not sufficient to stimulate the flounder heart.…”

Section: Catecholamine Dose-response Curves and Cardiac β-Adrenorecepmentioning

confidence: 74%

“…The punch-technique for the measurement of ventricular β-adrenoreceptors was performed as previously described by Gamperl et al (Gamperl et al, 1994a) for rainbow trout. Flounder (average mass 0.55±0.03 kg) were killed by a blow to the head, and the heart was quickly removed and allowed to beat for approximately 1 min in cold (0-2°C) saline to remove erythrocytes from the ventricular lumen.…”

Section: In Vitro Experiments Cardiac β-Adrenoreceptorsmentioning

confidence: 99%

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Nervous and humoral control of cardiac performance in the winter flounder(Pleuronectes americanus)

Mendonça

Gamperl

2009

Journal of Experimental Biology

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SUMMARYPrevious studies have suggested that flatfish lack adrenergic cardiac innervation and have a limited humoral adrenergic stress response. However, data on neurohormonal control of flatfish cardiac function is scarce, and has never been directly studied in vivo. Hence, we (1) injected neural and humoral antagonists into flounder (Pleuronectes americanus) in vivo to determine the contribution of autonomic innervation and circulating catecholamines to the control of resting cardiac function; (2) measured preand post-stress (90 s chase) catecholamine levels in this species; and (3) constructed in vivo catecholamine dose-response curves for cardiovascular function based on the results of the second experiment. In addition, we quantified the density (B max ) and ligand-binding affinity (K d ) of flounder ventricular cell-surface β-adrenoreceptors, and established whether they were of β 1 or β 2 subtype using pharmacological antagonists. The cholinergic contribution to resting flounder heart rate was comparable to other teleosts (cholinergic tonus 26%). Interestingly, however, bretylium increased heart rate, resulting in a negative resting adrenergic tonus (-11.9%), and we were unable to demonstrate that catecholamines supported cardiac function at rest or at circulating concentrations approximating those following an exhaustive chase (adrenaline, 21 nmol l ) for these receptors raises the possibility that the flounder heart is also populated by β 3 -adrenoreceptors.

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Section: Catecholamine Dose-response Curves and Cardiac β-Adrenorecepsupporting

confidence: 73%

Section: Catecholamine Dose-response Curves and Cardiac β-Adrenorecepmentioning

confidence: 74%

Section: In Vitro Experiments Cardiac β-Adrenoreceptorsmentioning

confidence: 99%

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Nervous and humoral control of cardiac performance in the winter flounder(Pleuronectes americanus)

Mendonça

Gamperl

2009

Journal of Experimental Biology

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“…CA exert a basal excitatory tone that prevails over the cholinergic tone. Adrenergic tone is mediated by a-and b-ARs associated with both the pacemaker and the working myocardium (Axelsson et al, 1987;Gamperl et al, 1994). Adrenergic stimulation increases f H (Graham and Farrell, 1989) and slightly improves the Frank-Starling response (Farrell et al, 1986).…”

Section: Adrenergic Neuroendocrine Patternsmentioning

confidence: 99%

Catecholamines, cardiac natriuretic peptides and chromogranin A: evolution and physiopathology of a ‘whip-brake’ system of the endocrine heart

Tota

Cerra

Gattuso

2010

Journal of Experimental Biology

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Summary In the past 50 years, extensive evidence has shown the ability of vertebrate cardiac non-neuronal cells to synthesize and release catecholamines (CA). This formed the mindset behind the search for the intrinsic endocrine heart properties, culminating in 1981 with the discovery of the natriuretic peptides (NP). CA and NP, co-existing in the endocrine secretion granules and acting as major cardiovascular regulators in health and disease, have become of great biomedical relevance for their potent diagnostic and therapeutic use. The concept of the endocrine heart was later enriched by the identification of a growing number of cardiac hormonal substances involved in organ modulation under normal and stress-induced conditions. Recently, chromogranin A (CgA), a major constituent of the secretory granules, and its derived cardio-suppressive and antiadrenergic peptides, vasostatin-1 and catestatin, were shown as new players in this framework, functioning as cardiac counter-regulators in ‘zero steady-state error’ homeostasis, particularly under intense excitatory stimuli, e.g. CA-induced myocardial stress. Here, we present evidence for the hypothesis that is gaining support, particularly among human cardiologists. The actions of CA, NP and CgA, we argue, may be viewed as a hallmark of the cardiac capacity to organize ‘whip-brake’ connection-integration processes in spatio-temporal networks. The involvement of the nitric oxide synthase (NOS)/nitric oxide (NO) system in this configuration is discussed. The use of fish and amphibian paradigms will illustrate the ways that incipient endocrine-humoral agents have evolved as components of cardiac molecular loops and important intermediates during evolutionary transitions, or in a distinct phylogenetic lineage, or under stress challenges. This may help to grasp the old evolutionary roots of these intracardiac endocrine/paracrine networks and how they have evolved from relatively less complicated designs. The latter can also be used as an intellectual tool to disentangle the experimental complexity of the mammalian and human endocrine hearts, suggesting future investigational avenues.

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“…A redistribution of cellular Ca 2+ is likely to be involved (Grossmann & Furchgott, 1964;Niedergerke & Page, 1977), as well as a stimulation of glycolysis (Williamson, 1964). The cardiac effects of adrenaline are mediated through a β-adrenoceptor signaling pathway that, in fish, involves β 2 -adrenoceptors (Ask et al, 1981;Temma et al, 1986;Gamperl et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Differences in Ca2+-management between the ventricle of two species of neotropical teleosts: the jeju, Hoplerythrinus unitaeniatus (Spix & Agassiz, 1829), and the acara, Geophagus brasiliensis (Quoy & Gaimard, 1824)

2009

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This study analyzed the physiological role of the cardiac sarcoplasmic reticulum (SR) of two neotropical teleosts, the jeju, Hoplerythrinus unitaeniatus (Erythrinidae), and the acara, Geophagus brasiliensis (Cichlidae). While the in vivo heart frequency (f H -bpm) of acara (79.6 ± 6.6) was higher than that of the jeju (50.3 ± 2.7), the opposite was observed for the ventricular inotropism (Fc -mN/mm 2 ) at 12 bpm (acara = 28.66 ± 1.86 vs. jeju = 36.09 ± 1.67). A 5 min diastolic pause resulted in a strong potentiation of Fc (≅ 90%) of strips from jeju, which was completely abolished by ryanodine. Ryanodine also resulted in a ≅ 20% decrease in the Fc developed by strips from jeju at both subphysiological (12 bpm) and physiological (in vivo) frequencies. However, this effect of ryanodine reducing the Fc from jeju was completely compensated by adrenaline increments (10 -9 and 10 -6 M). In contrast, strips from acara were irresponsive to ryanodine, irrespective of the stimulation frequency, and increases in adrenaline concentration (to 10 -9 and 10 -6 M) further increased Fc. These results reinforce the hypothesis of the functionality of the SR as a common trait in neotropical ostariophysian (as jeju), while in acanthopterygians (as acara) it seems to be functional mainly in 'athletic' species.

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β-Adrenoreceptors in the Trout (Oncorhynchus mykiss) Heart: Characterization, Quantification, and Effects of Repeated Catecholamine Exposure

Cited by 74 publications

References 0 publications

Nervous and humoral control of cardiac performance in the winter flounder(Pleuronectes americanus)

Nervous and humoral control of cardiac performance in the winter flounder(Pleuronectes americanus)

Catecholamines, cardiac natriuretic peptides and chromogranin A: evolution and physiopathology of a ‘whip-brake’ system of the endocrine heart

Differences in Ca2+-management between the ventricle of two species of neotropical teleosts: the jeju, Hoplerythrinus unitaeniatus (Spix & Agassiz, 1829), and the acara, Geophagus brasiliensis (Quoy & Gaimard, 1824)

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