Β-Amyrin and Benzene-1,2,4-Trimethyl from Euphorbia Hirta L. And Nauclea Latifolia (Smith) Leaves Induce Dauer Diapause Via Antagonist Inhibition of Daf-12 Receptor

Ogunmefun, O. T.; Ogunojemite, Oladotun Ayodeji; Adedokun, Oluwasegun; Olagbemide, Peter Taiwo; Kehinde, Ibrahim Oluwatobi; Soliman, Mahmoud E. S.

doi:10.2139/ssrn.4332211

Cited by 3 publications

(6 citation statements)

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“…The reported compounds belong to different classes as depicted in Tables and . Among these compounds, two compounds have been reported with antidiabetic , activities and five have been reported with antioxidant ,,, effects. In antioxidant compounds, 2,4-di- tert -butylphenol is a commercially available antioxidant as shown in Tables and .…”

Section: Discussionmentioning

confidence: 99%

Phytochemicals, Antioxidant, and Antidiabetic Effects of Ranunculus hirtellus Aerial Parts and Roots: Methanol and Aqueous Extracts

Ishtiaq,

Ahmad,

Zahid

et al. 2024

ACS Omega

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Ranunculus hirtellus, also known as crowfoot (buttercup), has a rich tradition of use in various biological contexts. While antibacterial studies on extracts from this plant have been conducted, the phytochemical composition, antioxidant properties, and antidiabetic effects remain unexplored. In this study, the phytochemical, antioxidant, and antidiabetic effects of its methanol and aqueous extracts were investigated. Our approach involved gas chromatography− mass spectrometry (GC/MS), alongside quantitative and qualitative methods, for phytochemical profiles. Additionally, concerning biological activities, the antioxidant effect was assessed through 2, 2-diphenyl-pieryl hydrazyl (DPPH) and 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) assays, while the antidiabetic effect was examined through the α-amylase inhibitory assay. The chloroform, ethyl acetate, and n-hexane extracts of R. hirtellus revealed the presence of 14 distinct compounds. In the methanol extract, sterols, quinones, glycosides, lactones, lignin, and flavonoids were identified. The aqueous extract contained sterols, alkaloids, glycosides, triterpenes, terpenoids, quinones, leucoanthocyanins, and lactones. The total flavonoid content (TFC), total phenolic content (TPC), total tannin content (TTC), and reducing sugar content (RDC) were determined in plant extracts, and a linear relationship was found between these parameters. Additionally, the TTC, TPC, and TFC values for both extracts hovered around 0.3786, 0.0476, and 0.1864 μg/mL, respectively, across all plant concentrations, while RDC ranged from 0.9336 to 1.0119 μg/mL in all four extracts. In vitro assays demonstrated dose-dependent antidiabetic activity in both methanolic and aqueous extracts by inhibiting α-amylase. Furthermore, the antioxidant activity observed in the DPPH assay was greater in the aqueous extract compared with the methanolic extract. In addition, the ethyl acetate extract exhibited the highest inhibition among chloroform and n-hexane in the ABTS assay. The results suggest that R. hirtellus can be a potential source of natural antioxidants and antidiabetic agents, and further studies are warranted to investigate the underlying mechanisms of its therapeutic effects.

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Section: Discussionmentioning

confidence: 99%

Phytochemicals, Antioxidant, and Antidiabetic Effects of Ranunculus hirtellus Aerial Parts and Roots: Methanol and Aqueous Extracts

Ishtiaq,

Ahmad,

Zahid

et al. 2024

ACS Omega

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“…A total of four compounds were detected in the GC-MS analysis of the chloroform extract of S. alba mangrove leaves, namely mesitylene, benzene-1,2,4-trimethyl, benzene-1-ethyl-3-methyl, and benzene-1-ethyl-2-methyl. Among those compounds, benzene-1-ethyl-3-methyl is known to have antifungal and antibacterial properties [28], while benzene-1,2,4-trimethyl may have potential as an anthelmintic [29]. However, their antioxidant activities are still unknown.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical and antioxidant activities of chloroform extract of Sonneratia alba leaves from Ngurah Rai Mangrove Forest, Bali - Indonesia

Wijaya,

Surya,

Udiyani

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The increasing prevalence of oxidative stress-related diseases triggers exploration of new antioxidant compounds. Among natural resources, mangrove trees are prominent producer of unique secondary metabolites. This research was designed to determine phytochemicals and antioxidant activity of Sonneratia alba mangrove leaves extract from Ngurah Rai Mangrove Forest, Bali. The study comprises experimental trials with post-test only control group design. Fresh leaves collected from five different S. alba plants were washed, dried, and grinded into powder. One-hundred gram of dried leaves powder was macerated with 500 mL of chloroform for 2x24 hours. The crude extract was analysed for its antioxidant activity based on DPPH assay. While, phytochemical analysis was evaluated using qualitative approaches and GC-MS method. The chloroform extract showed very strong antioxidant activity with IC50 of 15.59±0.78 ppm. Phytochemical analysis displayed that the extract contains tannin, phenol, and steroid, which explain its antioxidant properties. Furthermore, GC-MS analysis showed that this extract consists of four compounds, namely mesitylene, benzene-1,2,4-trimethyl, benzene-1-ethyl-3-methyl, and benzene-1-ethyl-2-methyl. In conclusion, these results present promising antioxidant activity of chloroform extract of S. alba mangrove leaves from Ngurah Rai Mangrove Forest, Bali. Further research is required to isolate and to purify the specific antioxidant bioactive for therapeutic purposes.

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“…A query of the chemical data set was obtained which consists of 8307 unique compounds for virtual screening. After preparing the compounds in AutoDockTool (ADT) and saving them in PDBQT format to allow compliance with the AutoDock Vina format, 34 …”

Section: Methodsmentioning

confidence: 99%

“…After preparing the compounds in AutoDockTool (ADT) and saving them in PDBQT format to allow compliance with the AutoDock Vina format,. 34…”

Section: Molecular-database: Selection and Preparationmentioning

confidence: 99%

“…Every 1 ps, the coordinates, and trajectories were created and examined using the AMBER18 GPU PTRAJ module. 34,36 After the molecular dynamic productions, the root mean square fluctuation (RMSF) and root mean square deviation (RMSD) of the PCSK9 protein complexes were determined to explore the system's flexibility and stability respectively. 37 To be able to explore system compactness, we estimated the protein systems' radius of gyration (RoG).…”

Section: Molecular Dynamic Simulationmentioning

confidence: 99%

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PCSK9 inhibitors as safer therapeutics for atherosclerotic cardiovascular disease (ASCVD): Pharmacophore design and molecular dynamics analysis

Kehinde,

Akawa,

Adewumi

et al. 2024

J of Cellular Biochemistry

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Cardiovascular disorders are still challenging and are among the deadly diseases. As a major risk factor for atherosclerotic cardiovascular disease, dyslipidemia, and high low‐density lipoprotein cholesterol in particular, can be prevented primary and secondary by lipid‐lowering medications. Therefore, insights are still needed into designing new drugs with minimal side effects. Proprotein convertase subtilisin/kexin 9 (PCSK9) enzyme catalyses protein‐protein interactions with low‐density lipoprotein, making it a critical target for designing promising inhibitors compared to statins. Therefore, we screened for potential compounds using a redesigned PCSK9 conformational behaviour to search for a significantly extensive chemical library and investigated the inhibitory mechanisms of the final compounds using integrated computational methods, from ligand essential functional group screening to all‐atoms MD simulations and MMGBSA‐based binding free energy. The inhibitory mechanisms of the screened compounds compared with the standard inhibitor. K31 and K34 molecules showed stronger interactions for PCSK9, having binding energy (kcal/mol) of −33.39 and −63.51, respectively, against −27.97 of control. The final molecules showed suitable drug‐likeness, non‐mutagenesis, permeability, and high solubility values. The C‐α atoms root mean square deviation and root mean square fluctuation of the bound‐PCSK9 complexes showed stable and lower fluctuations compared to apo PCSK9. The findings present a model that unravels the mechanism by which the final molecules proposedly inhibit the PCSK9 function and could further improve the design of novel drugs against cardiovascular diseases.

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Β-Amyrin and Benzene-1,2,4-Trimethyl from Euphorbia Hirta L. And Nauclea Latifolia (Smith) Leaves Induce Dauer Diapause Via Antagonist Inhibition of Daf-12 Receptor

Cited by 3 publications

References 0 publications

Phytochemicals, Antioxidant, and Antidiabetic Effects of Ranunculus hirtellus Aerial Parts and Roots: Methanol and Aqueous Extracts

Phytochemicals, Antioxidant, and Antidiabetic Effects of Ranunculus hirtellus Aerial Parts and Roots: Methanol and Aqueous Extracts

Phytochemical and antioxidant activities of chloroform extract of Sonneratia alba leaves from Ngurah Rai Mangrove Forest, Bali - Indonesia

PCSK9 inhibitors as safer therapeutics for atherosclerotic cardiovascular disease (ASCVD): Pharmacophore design and molecular dynamics analysis

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