β-Carotene and apocarotenals promote retinoid signaling in BEAS-2B human bronchioepithelial cells

Kuntz, Emmanuelle; Hoeller, Ulrich; Greatrix, Brad; Lankin, Christopher; Seifert, Nicole; Acharya, Samir; Riss, Georges; Buchwald-Hunziker, Petra; Hunziker, Willi; Góralczyk, Regina; Wertz, Karin

doi:10.1016/j.abb.2006.08.023

Cited by 10 publications

(7 citation statements)

References 30 publications

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“…In animals, ROS-induced oxidized carotenoid derivatives have been shown to be biologically active, playing a role in changes in gene expression, transcription activation, and apoptosis (e.g., refs. [30][31][32][33]. This study demonstrates that carotenoid oxidation products accumulating in light-stressed Arabidopsis plants are bioactive molecules: The expression of a large range of genes (>1,000) was changed in Arabidopsis plants exposed to β-CC.…”

Section: Discussionmentioning

confidence: 67%

Carotenoid oxidation products are stress signals that mediate gene responses to singlet oxygen in plants

Ramel

Birtić

Giniès

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

608

665

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1 O 2 (singlet oxygen) is a reactive O 2 species produced from triplet excited chlorophylls in the chloroplasts, especially when plants are exposed to excess light energy. Similarly to other active O 2 species, 1 O 2 has a dual effect: It is toxic, causing oxidation of biomolecules, and it can act as a signal molecule that leads to cell death or to acclimation. Carotenoids are considered to be the main 1 O 2 quenchers in chloroplasts, and we show here that light stress induces the oxidation of the carotenoid β-carotene in Arabidopsis plants, leading to the accumulation of different volatile derivatives. One such compound, β-cyclocitral, was found to induce changes in the expression of a large set of genes that have been identified as 1 O 2 responsive genes. In contrast, β-cyclocitral had little effect on the expression of H 2 O 2 gene markers. β-Cyclocitral–induced reprogramming of gene expression was associated with an increased tolerance to photooxidative stress. The results indicate that β-cyclocitral is a stress signal produced in high light that is able to induce defense mechanisms and represents a likely messenger involved in the 1 O 2 signaling pathway in plants.

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Section: Discussionmentioning

confidence: 67%

Carotenoid oxidation products are stress signals that mediate gene responses to singlet oxygen in plants

Ramel

Birtić

Giniès

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

608

665

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“…To determine the effect of Xrn2 on differentiation of a relevant lung epithelial cell type, we overexpressed Xrn2 in human Beas-2B cells. Beas-2B cells are of lung epithelial origin and retain the ability to be induced into squamous epithelial differentiation by 10% fetal calf serum (FCS) (Ke et al ., 1988; Kuntz et al ., 2006). To study differentiation, we used involucrin, a known marker of squamous epithelium and squamous differentiation in normal and tumor tissues including lung tumors (Said et al ., 1983; Walts et al ., 1985).…”

Section: Resultsmentioning

confidence: 99%

Genetic variants cis-regulating Xrn2 expression contribute to the risk of spontaneous lung tumor

Lü¹,

Liu²,

James³

et al. 2009

Oncogene

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Gene expression variation is an important mechanism underlying susceptibility to complex disease. In comparison with tobacco-related lung carcinogenesis, lung cancer in nonsmokers may involve important and etiologically distinct causal pathways. In this study, we conducted a genome-wide association study on spontaneous lung tumor incidence in inbred mice and identified a major susceptibility locus on mouse chromosome 2 (rs27328255, P = 6.68 × 10−7). We then evaluated the correlations of polymorphisms with the transcription of positional candidate genes in normal lungs. Single-nucleotide polymorphism rs27328255 was consistently and strongly associated (P = 7.42 × 10−9) in cis with transcript levels of Xrn2. We further showed that Xrn2 promotes proliferation and inhibits squamous differentiation in human lung epithelial cells and polymorphisms in human homolog XRN2 are associated with human lung cancer (rs2025811, P = 1.90 × 10−3, OR = 1.20). We conclude that genetic variants regulating Xrn2 expression in cis are determinants of spontaneous lung tumor susceptibility in mice and have genetic equivalents in lung cancer susceptibility in human beings. Identifying Xrn2 as a major candidate for spontaneous lung cancer has important implications for the diagnosis and treatment of lung cancer as well as delineation of the mechanisms underlying the genesis of lung cancer in nonsmokers.

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“…In addition, RES can conjugate with glutathione (Davoine et al, 2005), and some of the effects of RES may be related to the depletion of reductants. In animal cells, oxidized carotenoid derivatives have been reported to have a multitude of effects, including cytotoxicity (Lakshminarayana et al, 2010), induction of aptotosis (Janakiram et al, 2008;Kalariya et al, 2008;Liu et al, 2008), enzyme inactivation and inhibition of mitochondrial respiration (Siems et al, 2000(Siems et al, , 2002Hurst et al, 2005), induction of P450 cytochrome (Jeong et al, 1998), damage to DNA (Yeh and Wu, 2006;Kalariya et al, 2009) and retinoid signaling antagonism or promotion (Kuntz et al, 2006;Eroglu et al, 2012). In addition, carotenoid oxidation products, such as dihydroactinidiolide (Figure 1), are active components of pheromones in several insects (Rocca et al, 1983), and act as cat attractants in some plant species (Zhao et al, 2006).…”

Section: Carotenoid Oxidation Products Are Bioactivementioning

confidence: 99%

Carotenoid oxidation products as stress signals in plants

2013

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SUMMARYCarotenoids are known to play important roles in plants as antioxidants, accessory light-harvesting pigments, and attractants for pollinators and seed dispersers. A new function for carotenoids has recently emerged, which relates to the response of plants to environmental stresses. Reactive oxygen species, especially singlet oxygen, produced in the chloroplasts under stress conditions, can oxidize carotenoids leading to a variety of oxidized products, including aldehydes, ketones, endoperoxides and lactones. Some of those carotenoid derivatives, such as volatile b-cyclocitral, derived from the oxidation of b-carotene, are reactive electrophile species that are bioactive and can induce changes in gene expression leading to acclimation to stress conditions. This review summarizes the current knowledge on the non-enzymatic oxidation of carotenoids, the bioactivity of the resulting cleavage compounds and their functions as stress signals in plants.

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β-Carotene and apocarotenals promote retinoid signaling in BEAS-2B human bronchioepithelial cells

Cited by 10 publications

References 30 publications

Carotenoid oxidation products are stress signals that mediate gene responses to singlet oxygen in plants

Carotenoid oxidation products are stress signals that mediate gene responses to singlet oxygen in plants

Genetic variants cis-regulating Xrn2 expression contribute to the risk of spontaneous lung tumor

Carotenoid oxidation products as stress signals in plants

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