β-Catenin-Dependent Control of Positional Information along the AP Body Axis in Planarians Involves a Teashirt Family Member

Reuter, Hanna; März, Martin; Vogg, Matthias Christian; Eccles, David; Grífol-Boldú, Laura; Wehner, Daniel; Owlarn, Suthira; Adell, Teresa; Weidinger, Gilbert; Bartscherer, Kerstin

doi:10.1016/j.celrep.2014.12.018

Cited by 69 publications

(109 citation statements)

References 54 publications

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“…In addition to its role in posterior development, the canonical Wnt pathway appears to be implicated in the regeneration of the planarian head and brain (Fraguas et (Reuter et al, 2015), would depend on the action of the posterior Wnts (WNT1, WNT11-1, WNT11-2, WNT11-5). In the anterior region, β-CATENIN-1 is required for expression of notum among other effectors of anterior morphogenesis.…”

Section: β-Catenin-1 Controls Anterior Patterningmentioning

confidence: 99%

Localization of planarian β-CATENIN-1 reveals multiple roles during anterior-posterior regeneration and organogenesis

2016

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The β-catenin-dependent Wnt pathway exerts multiple contextdependent roles in embryonic and adult tissues. In planarians, β-catenin-1 is thought to specify posterior identities through the generation of an anteroposterior gradient. However, the existence of such a gradient has not been directly demonstrated. Here, we use a specific polyclonal antibody to demonstrate that nuclear β-CATENIN-1 exists as an anteroposterior gradient from the pre-pharyngeal region to the tail of the planarian Schmidtea polychroa. High levels in the posterior region steadily decrease towards the pre-pharyngeal region but then increase again in the head region. During regeneration, β-CATENIN-1 is nuclearized in both anterior and posterior blastemas, but the canonical WNT1 ligand only influences posterior nuclearization. Additionally, β-catenin-1 is required for proper anterior morphogenesis, consistent with the high levels of nuclear β-CATENIN-1 observed in this region. We further demonstrate that β-CATENIN-1 is abundant in developing and differentiated organs, and is particularly required for the specification of the germline. Altogether, our findings provide the first direct evidence of an anteroposterior nuclear β-CATENIN-1 gradient in adult planarians and uncover novel, context-dependent roles for β-catenin-1 during anterior regeneration and organogenesis.

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Section: β-Catenin-1 Controls Anterior Patterningmentioning

confidence: 99%

Localization of planarian β-CATENIN-1 reveals multiple roles during anterior-posterior regeneration and organogenesis

2016

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“…Analysis of the role of teashirt in planarians has also been described by Reuter et al (2015), published during final publication preparations of this manuscript.…”

Section: Note Added In Proofmentioning

confidence: 99%

teashirt is required for head-versus-tail regeneration polarity in planarians

et al. 2015

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Regeneration requires that the identities of new cells are properly specified to replace missing tissues. The Wnt signaling pathway serves a central role in specifying posterior cell fates during planarian regeneration. We identified a gene encoding a homolog of the Teashirt family of zinc-finger proteins in the planarian Schmidtea mediterranea to be a target of Wnt signaling in intact animals and at posterior-facing wounds. Inhibition of Smed-teashirt (teashirt) by RNA interference (RNAi) resulted in the regeneration of heads in place of tails, a phenotype previously observed with RNAi of the Wnt pathway genes β-catenin-1, wnt1, Dvl-1/2 or wntless. teashirt was required for β-catenin-1-dependent activation of posterior genes during regeneration. These findings identify teashirt as a transcriptional target of Wnt signaling required for Wnt-mediated specification of posterior blastemas.

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“…Paraffin embedding, sectioning, and subsequent in situ hybridization and immunostaining were performed as previously described (Adell et al, in press;Cardona et al, 2005a). The antibodies used were rabbit anti-SMEDWI-1 (1:1100), mouse anti-MHC (kindly provided by Rafael Romero, University of Barcelona, Spain; 1:20) (Cebrià et al, 1997), anti-phospho-histone-H3-Ser10 (anti-PH3) (Cell Signaling, #3377S; 1:300) and rabbit anti-RPZ-1 (kindly provided by Kerstin Bartscherer; 1:200) (Reuter et al, 2015). Mallory's staining was performed as previously described (Cardona et al, 2005b;Sluys, 1989).…”

Section: Whole-mount In Situ Hybridization and Immunohistochemistrymentioning

confidence: 99%

“…4E). To determine whether cell loss affected one or both gastrodermal cell types, we used an anti-RPZ-1 antibody to specifically label goblet cells (Reuter et al, 2015) (Fig. 4F).…”

Section: Egfr-1 Silencing Affects Gastrodermis Integritymentioning

confidence: 99%

The EGFR signaling pathway controls gut progenitor differentiation during planarian regeneration and homeostasis

2016

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The planarian Schmidtea mediterranea maintains and regenerates all its adult tissues through the proliferation and differentiation of a single population of pluripotent adult stem cells (ASCs) called neoblasts. Despite recent advances, the mechanisms regulating ASC differentiation into mature cell types are poorly understood. Here, we show that silencing of the planarian EGF receptor egfr-1 by RNA interference (RNAi) impairs gut progenitor differentiation into mature cells, compromising gut regeneration and maintenance. We identify a new putative EGF ligand, nrg-1, the silencing of which phenocopies the defects observed in egfr-1(RNAi) animals. These findings indicate that egfr-1 and nrg-1 promote gut progenitor differentiation, and are thus essential for normal cell turnover and regeneration in the planarian gut. Our study demonstrates that the EGFR signaling pathway is an important regulator of ASC differentiation in planarians.

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β-Catenin-Dependent Control of Positional Information along the AP Body Axis in Planarians Involves a Teashirt Family Member

Cited by 69 publications

References 54 publications

Localization of planarian β-CATENIN-1 reveals multiple roles during anterior-posterior regeneration and organogenesis

Localization of planarian β-CATENIN-1 reveals multiple roles during anterior-posterior regeneration and organogenesis

teashirt is required for head-versus-tail regeneration polarity in planarians

The EGFR signaling pathway controls gut progenitor differentiation during planarian regeneration and homeostasis

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