β-chain of ATP synthase as a lipophorin binding protein and its role in lipid transfer in the midgut of Panstrongylus megistus (Hemiptera: Reduviidae)

Fruttero, Leonardo L.; Demartini, Diogo Ribeiro; Rubiolo, Edilberto R.; Carlini, Célia R.; Canavoso, Lilián Etelvina

doi:10.1016/j.ibmb.2014.06.002

Cited by 20 publications

(15 citation statements)

References 52 publications

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“…3 ). Although previously thought to only have a role in the mitochondria, ATPases are present in the plasma membrane of the insect midgut and salivary glands and have been shown to have a role in lipid transport 35 , 36 .…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic meta-signatures identified in Anopheles gambiae populations reveal previously undetected insecticide resistance mechanisms

2018

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Increasing insecticide resistance in malaria-transmitting vectors represents a public health threat, but underlying mechanisms are poorly understood. Here, a data integration approach is used to analyse transcriptomic data from comparisons of insecticide resistant and susceptible Anopheles populations from disparate geographical regions across the African continent. An unbiased, integrated analysis of this data confirms previously described resistance candidates but also identifies multiple novel genes involving alternative resistance mechanisms, including sequestration, and transcription factors regulating multiple downstream effector genes, which are validated by gene silencing. The integrated datasets can be interrogated with a bespoke Shiny R script, deployed as an interactive web-based application, that maps the expression of resistance candidates and identifies co-regulated transcripts that may give clues to the function of novel resistance-associated genes.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic meta-signatures identified in Anopheles gambiae populations reveal previously undetected insecticide resistance mechanisms

2018

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“…Apolipophorin has a long half-life, calculated to exceed one day in some species [ 6 ], and each particle participates in multiple cycles of lipid loading and unloading in tissues without apparent degradation of the Apolipophorin moiety [ 7 ]. Biochemical and kinetic studies indicated that the interaction of lipophorin with cells is mediated through specific receptors [ 8 – 11 ]. At the molecular level, the best characterized are the lipophorin receptors of the Low Density Lipoprotein Receptor (LDLR) family, which were initially identified by their capacity to induce lipophorin endocytosis when overexpressed in a cell culture system [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Drosophila Lipophorin Receptors Recruit the Lipoprotein LTP to the Plasma Membrane to Mediate Lipid Uptake

et al. 2015

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Lipophorin, the main Drosophila lipoprotein, circulates in the hemolymph transporting lipids between organs following routes that must adapt to changing physiological requirements. Lipophorin receptors expressed in developmentally dynamic patterns in tissues such as imaginal discs, oenocytes and ovaries control the timing and tissular distribution of lipid uptake. Using an affinity purification strategy, we identified a novel ligand for the lipophorin receptors, the circulating lipoprotein Lipid Transfer Particle (LTP). We show that specific isoforms of the lipophorin receptors mediate the extracellular accumulation of LTP in imaginal discs and ovaries. The interaction requires the LA-1 module in the lipophorin receptors and is strengthened by a contiguous region of 16 conserved amino acids. Lipophorin receptor variants that do not interact with LTP cannot mediate lipid uptake, revealing an essential role of LTP in the process. In addition, we show that lipophorin associates with the lipophorin receptors and with the extracellular matrix through weak interactions. However, during lipophorin receptor-mediated lipid uptake, LTP is required for a transient stabilization of lipophorin in the basolateral plasma membrane of imaginal disc cells. Together, our data suggests a molecular mechanism by which the lipophorin receptors tether LTP to the plasma membrane in lipid acceptor tissues. LTP would interact with lipophorin particles adsorbed to the extracellular matrix and with the plasma membrane, catalyzing the exchange of lipids between them.

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“…The mechanism of Stunted release remains to be clarified. The beta subunit of the ectopic form of F 1 F 0 -ATP synthase is a receptor for lipoproteins (18)(19)(20)(21), which serve as cargos for proteins and peptides. In addition, Drosophila lipid transfer particle-containing lipoproteins were shown to act on the larval brain to control systemic insulin signaling in response to nutrition (23).…”

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confidence: 99%

Drosophila insulin release is triggered by adipose Stunted ligand to brain Methuselah receptor

Delanoue

Meschi

Agrawal

et al. 2016

Science

166

142

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Animals adapt their growth rate and body size to available nutrients by a general modulation of insulin-insulin-like growth factor signaling. In Drosophila, dietary amino acids promote the release in the hemolymph of brain insulin-like peptides (Dilps), which in turn activate systemic organ growth. Dilp secretion by insulin-producing cells involves a relay through unknown cytokines produced by fat cells. Here, we identify Methuselah (Mth) as a secretin-incretin receptor subfamily member required in the insulin-producing cells for proper nutrient coupling. We further show, using genetic and ex vivo organ culture experiments, that the Mth ligand Stunted (Sun) is a circulating insulinotropic peptide produced by fat cells. Therefore, Sun and Mth define a new cross-organ circuitry that modulates physiological insulin levels in response to nutrients.

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β-chain of ATP synthase as a lipophorin binding protein and its role in lipid transfer in the midgut of Panstrongylus megistus (Hemiptera: Reduviidae)

Cited by 20 publications

References 52 publications

Transcriptomic meta-signatures identified in Anopheles gambiae populations reveal previously undetected insecticide resistance mechanisms

Transcriptomic meta-signatures identified in Anopheles gambiae populations reveal previously undetected insecticide resistance mechanisms

Drosophila Lipophorin Receptors Recruit the Lipoprotein LTP to the Plasma Membrane to Mediate Lipid Uptake

Drosophila insulin release is triggered by adipose Stunted ligand to brain Methuselah receptor

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