β-Conglycinin (7S) and glycinin (11S) exert a hypocholesterolemic effect comparable to that of fenofibrate in rats fed a high-cholesterol diet

Abstract

Glycinin Fenofibrate Hypercholesterolemic diet Dyslipidemia Rats A B S T R A C TThe hypocholesterolemic effect of isolated soybean proteins and fenofibrate in rats was compared. Forty-five rats were divided into five groups: standard (STD; casein), high cholesterol (HC; STD plus 1% cholesterol/0.5% cholic acid), HC + b-conglycinin, HC + glycinin and HC + fenofibrate. The proteins and the drug were administered by gavage for 28 days. The proteins decreased total cholesterol (TC) and triacylglycerol (TG) in the plasma of the rats fed HC diet, to values very close to those fed on fenofibrate. High density lipoprotein cholesterol (HDL-C) levels in the plasma were increased by the b-conglycinin, glycinin and fenofibrate groups. The largest TC reduction in the liver was observed in the fenofibrate group; in contrast, the b-conglycinin and glycinin groups exhibited reduced the levels of hepatic TG and TC. Based on these data, it could be suggested that the oral daily administration of isolated soybean proteins, in the range of 2.75% of the protein ingested daily, can promote a reduction in TC and TG in the plasma of rats fed hypercholesterolemic diets.Crown

β-conglycinin combined with fenofibrate or rosuvastatin have exerted distinct hypocholesterolemic effects in rats

“…The isolation procedure did not cause any change in the subunits and was important to obtain purified protein suitable for the in vivo studies [14,15]. Our previous results and those decribed by Duranti et al [13] have shown that the addition of 1% cholesterol and 0.5% cholic acid to the AIN-diet is sufficient to induce hypercholesterolemia and hypertriglyceridemia in rats.…”

“…Thus, the atherogenic index (AI) of the hypercholesterolemic diet group (HC), in this experiment, was 8 times higher than that of rats on the casein standard diet [14], as also observed by other authors [10]. All treatments resulted in reductions in AI of the animals, the combination of 7S protein and fenofibrate (HC+7S+FF group) being the most efficient, followed by fenofibrate (HC+FF group), protein (HC+7S group), protein plus rosuvastatin (HC+7S+RO group) and rosuvastatin (HC+RO group), with falls of 81, 76, 57, 55 and 50%, relative to HC, respectively (Table 4).…”

Metformin and soybean-derived bioactive molecules attenuate the expansion of stem cell-like epithelial subpopulation and confer apoptotic sensitivity in human colon cancer cells

“…The extract was evaporated to dryness at room temperature and used for isolation of b-conglycinin and glycinin as previously described (Ferreira et al 2010). …”

IAVPGEVA, IAVPTGVA, and LPYP, three peptides from soy glycinin, modulate cholesterol metabolism in HepG2 cells through the activation of the LDLR-SREBP2 pathway

“…In particular, numerous studies have shown that soy foods are able to reduce total and lowdensity lipoprotein (LDL) cholesterol levels in highly and mildly hypercholesterolaemic subjects (Harland & Haffner, 2008;Jenkins et al, 2010;Sirtori, Eberini, & Arnoldi, 2007). Either in animals (Ferreira, Silva, Demonte, & Neves, 2010;Marazza, LeBlanc, Savoy de Giori, & Garro, 2013;Sirtori et al, 1984) or humans (Baum et al, 1998;Lovati et al, 1987), it has been demonstrated that this activity is linked to the modulation of LDL-receptors (LDLR), but the actual mechanism of action has not yet been understood in detail. Although isoflavones were initially considered the main factors responsible for the observed activity (Sirtori, Arnoldi, & Johnson, 2005), some following studies have addressed the possible role of soy proteins per se in cholesterolaemia reduction (Fukui et al, 2002;Liu et al, 2014).…”