β-Conglycinin enhances autophagy in porcine enterocytes

Hou, Yongqing; Mei, Huimin; Wang, Lei; Hu, Chien‐An Andy; Wu, Guoyao

doi:10.1007/s00726-016-2352-7

Cited by 5 publications

(4 citation statements)

References 18 publications

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“…This finding provides an important clue into the underlying mechanism whereby β-CG exerts adverse effects, although alteration in autophagic activity needs to be directly measured in future studies. Yi et al 11 reported that β-CG enhanced enterocyte autophagy in vitro. To date, there is little information on the relationship between β-CG and intestinal autophagy.…”

Section: Discussionmentioning

confidence: 99%

N-Acetylcysteine improves intestinal function and attenuates intestinal autophagy in piglets challenged with β-conglycinin

Wang

Peng

et al. 2021

Sci Rep

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Abstractβ-Conglycinin (β-CG), an anti-nutritional factor, is a major allergen in soybeans to induce intestinal dysfunction and diarrhea in neonatal animals, including piglets and human infants. This study with a piglet model determined the effects of N-acetylcysteine (NAC) on intestinal function and autophagy in response to β-CG challenge. Twenty-four 12-day-old piglets (3.44 ± 0.28 kg), which had been weaned at 7 days of age and adapted for 5 days after weaning, were randomly allocated to the control, β-CG, and β-CG + NAC groups. Piglets in the control group were fed a liquid diet containing 10% casein, whereas those in the β-CG and β-CG + NAC groups were fed the basal liquid diets containing 9.5% casein and 0.5% β-CG for 2 days. Thereafter, pigs in the β-CG + NAC group were orally administrated with 50 mg (kg BW)−1 NAC for 3 days, while pigs in the other two groups were orally administrated with the same volume of sterile saline. NAC numerically reduced diarrhea incidence (− 46.2%) and the concentrations of hydrogen peroxide and malondialdehyde, but increased claudin-1 and intestinal fatty-acid binding protein (iFABP) protein abundances and activities of catalase and glutathione peroxidase in the jejunum of β-CG-challenged piglets. Although β-CG challenge decreased the villus height, villus height/crypt depth ratio, and mRNA levels of claudin-1 and occludin, no significant differences were observed in these indices between the control and β-CG + NAC groups, suggesting the positive effects of NAC supplementation on intestinal mucosal barrier function. Moreover, NAC increased the concentrations of citrulline and D-xylose in the plasma, as well as the expression of genes for aquaporin (AQP) 3, AQP4, peptide transporter 1 (PepT1), sodium/glucose co-transporter-1 (SGLT-1), potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), and solute carrier family 1 member 1 (SLC1A1) in the jejunum, demonstrating that NAC augmented intestinal metabolic activity and absorptive function. Remarkably, NAC decreased Atg5 protein abundance and the LC3II/LC3I ratio (an indicator of autophagy) in the jejunum of β-CG-challenged piglets. Taken together, NAC supplementation improved intestinal function and attenuated intestinal autophagy in β-CG-challenged piglets.

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Section: Discussionmentioning

confidence: 99%

N-Acetylcysteine improves intestinal function and attenuates intestinal autophagy in piglets challenged with β-conglycinin

Wang

Peng

et al. 2021

Sci Rep

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“…9 In addition, β-CG enhanced LC3-II expression and autophagy in IPEC-1 cells. 7 Although these studies have provided evidence that several downstream pathways are implicated in β-CG-induced intestinal injury in pigs, the underlying regulatory mechanisms are not fully understood. A previous study analyzed the proteomic expression profile of β-CG-induced porcine intestinal cells using twodimensional gel electrophoresis and reported that 14 proteins were differentially expressed.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Peng et al found that β-CG decreased tight junction distribution and promoted cell death in porcine intestinal epithelial cells via the MAPK/NF-κB signaling pathway . In addition, β-CG enhanced LC3-II expression and autophagy in IPEC-1 cells . Although these studies have provided evidence that several downstream pathways are implicated in β-CG-induced intestinal injury in pigs, the underlying regulatory mechanisms are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

“…Studies have established that β-conglycinin (β-CG) is a highly allergenic protein in soybean that accounts for approximately 30% of soybean globulin. , β-CG primarily consists of three homologous subunits: α′, α, and β . Previous studies have reported that β-CG affects functional and morphological alterations in the intestine, including antioxidant enzyme levels, autophagy, , inflammatory cytokines, and epithelial cell injury . Therefore, understanding the mechanisms of β-CG-induced intestinal damage could provide an effective strategy for the application of soy products in piglet feed.…”

Section: Introductionmentioning

confidence: 99%

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Integrated Transcriptome and Proteome Analyses of β-Conglycinin-Induced Intestinal Damage in Piglets

Li,

Zheng,

Yin

et al. 2024

J. Agric. Food Chem.

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β-conglycinin (β-CG) induces intestinal damage in piglets; however, its regulatory mechanisms are not fully understood. This study aimed to investigate the molecular mechanisms by which β-CG regulates intestinal injury in piglets through downstream genes and proteins. Our findings revealed that β-CG significantly reduced villus height while increasing the crypt depth. In addition, we analyzed the transcriptome and proteome of jejunum tissues after the β-CG treatment. In total, 382 differentially expressed genes (DEGs) and 292 differentially expressed proteins (DEPs) were identified between the treatment and the control groups. The expression levels of DEGs and DEPs were validated by using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting, respectively. The findings revealed a consistent correlation between their expression levels and transcriptomic and proteomic data. In addition, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of DEGs and DEPs revealed their enrichment in oxidation-related GOs, as well as in lysosome-related pathways. A protein−protein interaction (PPI) regulatory network was constructed based on the DEPs. The integration of transcriptomic and proteomic analyses identified six genes that were significantly different at both the transcript and the protein levels. This study provides valuable insights into the molecular mechanisms underlying β-CG-induced intestinal injury in piglets.

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A novel highly sensitive soy aptasensor for antigen β-conglycinin determination

Wei

et al. 2021

Anal. Methods

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β-Conglycinin enhances autophagy in porcine enterocytes

Cited by 5 publications

References 18 publications

N-Acetylcysteine improves intestinal function and attenuates intestinal autophagy in piglets challenged with β-conglycinin

N-Acetylcysteine improves intestinal function and attenuates intestinal autophagy in piglets challenged with β-conglycinin

Integrated Transcriptome and Proteome Analyses of β-Conglycinin-Induced Intestinal Damage in Piglets

A novel highly sensitive soy aptasensor for antigen β-conglycinin determination

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