β‐cyclodextrin‐ionic liquid polymer based dynamically coating for simultaneous determination of tetracyclines by capillary electrophoresis

Abstract

Tetracyclines are a group of broad spectrum antibiotics widely used in animal husbandry to prevent and treat diseases. However, the improper use of tetracyclines may result in the presence of their residues in animal tissues or waste. Recently, great attention has been drawn towards the green solvents ionic liquids. Ionic liquids have been employed as a coating material to modify the electroosmotic flow in capillary electrophoresis. In this study, a functionalized ionic liquid, mono-6-deoxy-6-(3-methylimidazolium)-β-cyclodextrin tosylate, was synthesized and used for the simultaneous separation and quantification of tetracyclines by capillary electrophoresis. Good separation efficiency could be achieved due to the multiple functions of β-cyclodextrin derived ionic liquid, including the electrostatic interaction, the hydrogen bonding, and the cavity structure in β-cyclodextrin ionic liquid which can entrap the tetracyclines to form inclusion complex. After optimization, baseline separation achieved in 25 min with the running buffer consisted of 10 mmol/L, pH 7.2 phosphate buffer and 20 mmol/L β-cyclodextrin ionic liquid. The satisfied result demonstrated that the β-cyclodextrin ionic liquid is an ideal background electrolyte modifier in the separation of tetracyclines with high stability and good reproducibility. And it is an effective strategy to design and synthesize specific ILs as additive applied in separation.

Advances in the Analysis of Veterinary Drug Residues in Food Matrices by Capillary Electrophoresis Techniques

“…In order to improve CE sensitivity, a functionalized β-cyclodextrin-ionic liquid was added in-line; unlike the conventional β-cyclodextrin, it acted as an additive able to form a complex with TCs and as a capillary coating agent. The method proposed by Zhou et al was developed to separate four TCs in about 30 min by using CE with amperometric detection (AD) [66].…”

Analysis of antibiotics by CE and CEC and their use as chiral selectors: An update

“…In addition to UV and MS detection, other detection systems have been used in CE in the period of time reviewed in this article with the aim of determining different types of antibiotics. LIF , light emitting diode‐induced fluorescence detector (LED‐FLD), , photoluminescence , chemiluminescence , ECL , amperometric detection (AD) , and C 4 D were employed (see Table ).…”

“…Zhou et al. developed a simple and economical CE‐AD method for the analysis of four tetracycline antibiotics in water samples . A novel synthesized functionalized ionic liquid (mono‐6‐deoxy‐6‐(3‐methylimidazolium)‐β‐cyclodextrin) was employed for the simultaneous separation and quantitation of doxycycline, tetracycline, oxytetracycline, and chlortetracycline.…”

Solid-Phase Extraction and Large-Volume Sample Stacking-Capillary Electrophoresis for Determination of Tetracycline Residues in Milk

“…In recent years, due to the concerns caused by veterinary drugs contained in food samples, there were developed a large variety of analytical methodologies for the determination of TC residues at μ g·kg −1 or μ g·L −1 levels in different matrices. These methods included chemiluminescence [ 33 ], microbiological assays [ 34 ], high-performance liquid chromatography (HPLC) [ 35 , 36 ], or capillary electrophoresis (CE) [ 37 ].…”