β-Dihydroagarofuran-Type Sesquiterpenes from the Seeds of Celastrus monospermus and Their Lifespan-Extending Effects on the Nematode Caenorhabditis elegans

Abstract

Seventeen β-dihydroagarofuran-type sesquiterpenes were isolated from the seeds of Celastrus monospermus, and, among them, 15 (1-15) were identified as new natural products. Nine isolates were evaluated for their lifespan-extending effect using the standard model animal nematode Caenorhabditis elegans. As a result, all of the tested compounds prolonged the lifespan of C. elegans when compared to the blank control group (p < 0.0001). Among them, celaspermin E (5) extended the average lifespan and maximum lifespan of C. elegans, with effects similar to those of rapamycin, a positive control that has been found experimentally to delay the aging process of yeasts, worms, fruit flies, and mice.

l-Ascorbyl 2,6-dipalmitate inhibits biofilm formation and virulence in methicillin-resistant Staphylococcus aureus and prevents triacylglyceride accumulation in Caenorhabditis elegans

“…The worms were observed under an inverted microscope and the number of viable worms was counted every 24 h. The animals were considered to be dead when they did not respond to gentle tapping or touching with a platinum worm picker loop. 38…”

Dihydro-β-agarofurans from the roots of the Australian endemic rainforest tree Maytenus bilocularis act as leucine transport inhibitors

“…The relative configuration of 4 was assigned following analysis of 1 H-1 H coupling constants and the ROESY spectrum ( Figure 2). The β-orientation of H-1 and H-2 was assigned based on the small coupling constant between the two protons (J1,2 = 3.5 Hz) (Gao, et al, 2016;Núñez, et al, 2016). The ROESY spectrum of 4 exhibited correlations between H-1 and H-3β, indicating that these two atoms were co-facial.…”

Two New β-Dihydroagarofuran Sesquiterpenes from Celastrus orbiculatus Thunb and Their Anti-Proliferative Activity

“…1β,6α-diacetoxy-9α-benzoyloxy-β-dihydroagarofuran ( 3 ) [ 19 ], 1β-acetoxy-6α,9α-dibenzoyloxy-β-dihydroagarofuran ( 4 ) [ 19 ], 1β-acetoxy-9α-cinnamoyloxy-β-dihydroagarofuran ( 5 ) [ 20 ], 1β,6α,8β-triacetoxy-9α-benzoyloxy-β-dihydroagarofuran ( 6 ) [ 19 ], 1β,8α-diacetoxy-9α-cinnamoyloxy-β-dihydroagarofuran ( 7 ) [ 21 ], 1β,2β-diacetoxy-9α-cinnamoyloxy-β-dihydroagarofuran ( 8 ) [ 22 ], 1β,6α-diacetoxy-9α-cinnamoyloxy-β-dihydroagarofuran ( 9 ) [ 20 ], 1β,6α,13-triacetoxy-9α-benzoyloxy-β-dihydroagarofuran ( 10 ) [ 15 ], 1β-acetoxy-6α-cinnamoyloxy-9α-benzoyloxy-β-dihydroagarofuran ( 11 ) [ 23 ], 1β,2β,13-triacetoxy-9α-cinnamoyloxy-β-dihydroagarofuran ( 12 ) [ 24 ], 1β,2β,6α-triacetoxy-9α-cinnamoyloxy-β-dihydroagarofuran ( 13 ) [ 24 ], 1β,2β,6β-triacetoxy-9β-cinnamoyloxy-β-dihydroagarofuran ( 14 ) [ 25 ], 1β,2β,6α-triacetoxy-9α-benzoyloxy-β-dihydroagarofuran ( 15 ) [ 26 ], 1β-acetoxy-6α-hydroxydihydro-9α-benzoyloxy-β-dihydroagarofuran ( 16 ) [ 23 ]. 1β,6α-diacetoxy-8β-hydroxydihydro-9α-benzoyloxy-β-dihydroagarofuran ( 17 ) [ 27 ], 1β-cinnamyloxy-6α-acetoxy-8β-hydroxydihydro-9β-benzoyloxy-β-agarofuran ( 18 ) [ 23 ], 1β,8β-dihydroxy-6α-acetoxy-9β-benzoyloxy-β-dihydroagarofuran ( 19 ) [ 23 ] were isolated from the fruit of C .…”