β-galactosidase covalent immobilization over large-pore mesoporous silica supports for the production of high galacto-oligosaccharides (GOS)

González-Delgado, Isabel; Segura, Yolanda; Martín, Adrián; López-Muñoz, Marı́a José; Morales, Gabriel

doi:10.1016/j.micromeso.2017.08.020

Cited by 30 publications

(8 citation statements)

References 53 publications

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“…Except the above‐discussed functionalities, the other functional groups such as glycidyloxy, azido, isocyanate, cyano, vinyl, pyridyl substituted thioether, pyridyl, carboxylic acid, and phosphonate groups are grafted on silica through the condensation with the corresponding organosilanes. J. T. Sarmiento et al.…”

Section: Conventional Fss: Types and Their Chemical Modificationsmentioning

confidence: 99%

“…On the other hand, to anchor an enzyme (β‐galactosidase), the grafted epoxy groups are converted to aldehyde groups by the acid catalysed hydrolysis followed by the oxidation. The formed aldehyde groups are used to graft β‐galactosidase on silica by forming the imine linkage . Azide groups grafted on silica have taken part in the click reaction with alkyne molecules in presence of Cu(I) or Cu(II) catalyst, leading to the five membered ring systems containing heteroatoms.…”

Section: Conventional Fss: Types and Their Chemical Modificationsmentioning

confidence: 99%

“…As far as the enzymes immobilized on FSs concerned, they are catalytically active in diverse reactions including esterification, chemical syntheses, hydrolysis, oxidation, dyes degradation, deacetylation, protein digestion, resolution of racemic compounds . The immobilized enzymes have shown better performance than free enzymes in chosen catalytic reactions . FSs with relatively large pores and hydrophobic groups like phenyl or ethyl in the backbone have promoted the activity of immobilized enzymes.…”

Section: Applications and Advantages Of Fs‐based Catalystsmentioning

confidence: 99%

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Functionalized Silicas for Metal‐Free and Metal‐Based Catalytic Applications: A Review in Perspective of Green Chemistry

Antony

Marimuthu

Yang

et al. 2019

The Chemical Record

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Heterogeneous catalysis plays a key role in promoting green chemistry through many routes. The functionalizable reactive silanols highlight silica as a beguiling support for the preparation of heterogeneous catalysts. Metal active sites anchored on functionalized silica (FS) usually demonstrate the better dispersion and stability due to their firm chemical interaction with FSs. Having certain functional groups in structure, FSs can act as the useful catalysts for few organic reactions even without the need of metal active sites which are termed as the covetous reusable organocatalysts. Magnetic FSs have laid the platform where the effortless recovery of catalysts is realized just using an external magnet, resulting in the simplified reaction procedure. Using FSs of multiple functional groups, we can envisage the shortened reaction pathway and, reduced chemical uses and chemical wastes. Unstable bio‐molecules like enzymes have been stabilized when they get chemically anchored on FSs. The resultant solid bio‐catalysts exhibited very good reusability in many catalytic reactions. Getting provoked from the green chemistry aspects and benefits of FS‐based catalysts, we confer the recent literature and progress focusing on the significance of FSs in heterogeneous catalysis. This review covers the preparative methods, types and catalytic applications of FSs. A special emphasis is given to the metal‐free FS catalysts, multiple FS‐based catalysts and magnetic FSs. Through this review, we presume that the contribution of FSs to green chemistry can be well understood. The future perspective of FSs and the improvements still required for implementing FS‐based catalysts in practical applications have been narrated at the end of this review.

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Section: Conventional Fss: Types and Their Chemical Modificationsmentioning

confidence: 99%

Section: Conventional Fss: Types and Their Chemical Modificationsmentioning

confidence: 99%

Section: Applications and Advantages Of Fs‐based Catalystsmentioning

confidence: 99%

See 1 more Smart Citation

Functionalized Silicas for Metal‐Free and Metal‐Based Catalytic Applications: A Review in Perspective of Green Chemistry

Antony

Marimuthu

Yang

et al. 2019

The Chemical Record

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“…To overcome the shortcomings of the systems described above, we developed a PMMA/silica microparticulate composite employing a pharmaceutical grade, reproducibly manufactured, commercially available fumed precipitated mesoporous silica to act as a drug‐laden porosigen. These silica microparticles (average diameter 7.5 µm) have a randomly arranged, cage‐like pore structure with an average pore diameter of 35 nm and a pore volume of 1.23 cm 3 /gm 28,29 . The combination of larger pore diameter and cage‐like pore geometry not only enables the accommodation of larger diameter drug molecules such as vancomycin, but also translates to a higher drug loading and faster release rate of drug, 30,31 a desirable attribute to achieve bone concentrations well above the minimum inhibitory concentration (MIC) of the infecting organism.…”

Section: Introductionmentioning

confidence: 99%

“…These silica microparticles (average diameter 7.5 µm) have a randomly arranged, cage-like pore structure with an average pore diameter of 35 nm and a pore volume of 1.23 cm 3 /gm. 28,29 The combination of larger pore diameter and cage-like pore geometry not only enables the accommodation of larger diameter drug molecules such as vancomycin, but also translates to a higher drug loading and faster release rate of drug, 30,31 a desirable attribute to achieve bone concentrations well above the minimum inhibitory concentration (MIC) of the infecting organism.…”

Section: Introductionmentioning

confidence: 99%

Novel microcomposite implant for the controlled delivery of antibiotics in the treatment of osteomyelitis following total joint replacement

İnceoğlu

Botimer

Maskiewicz

2020

Journal Orthopaedic Research

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The objective of this study was to develop a novel microcomposite implant to be used in the treatment of osteomyelitis following total joint arthroplasty, with the dual purpose of releasing high local concentrations of antibiotic to eradicate the infection while providing adequate mechanical strength to maintain the dynamic or static spacer. Vancomycin‐loaded microcomposite implants were fabricated by incorporating drug‐loaded microparticles comprised of mesoporous silica into commonly employed polymethylmethacrylate (PMMA) bone cement, to yield a final drug loading of 10% w/w. In vitro release kinetics at 37°C were monitored by reverse‐phase high‐performance liquid chromatography, and compared to the release kinetics of current therapy implants consisting of drug alone incorporated at 10% w/w directly into PMMA bone cement. Results demonstrated a sevenfold improvement in the elution profile of microcomposite systems over current therapy implants. In vivo delivery of vancomycin to bone from microcomposite implants (70% of payload) was significantly higher than that from current therapy implants (approx. 22% of payload) and maintained significantly higher bone concentrations for up to 2 weeks duration. The elastic modulus showed no statistical difference between microcomposite implants and current standard therapy implants before drug elution, and maintenance of acceptable strength of microcomposite implants postdrug elution. These results demonstrate that we have developed a novel microcomposite spacer that will release continuously high antibiotic concentrations over a prolonged period of time, offering the possibility to eliminate infection and avoid the emergence of new resistant bacterial strains, while maintaining the requisite mechanical properties for proper space maintenance and joint fixation.

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Galactooligosaccharides as Potential Prebiotics

Kaur

Panesar

2022

Probiotics, Prebiotics and Synbiotics

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β-galactosidase covalent immobilization over large-pore mesoporous silica supports for the production of high galacto-oligosaccharides (GOS)

Cited by 30 publications

References 53 publications

Functionalized Silicas for Metal‐Free and Metal‐Based Catalytic Applications: A Review in Perspective of Green Chemistry

Functionalized Silicas for Metal‐Free and Metal‐Based Catalytic Applications: A Review in Perspective of Green Chemistry

Novel microcomposite implant for the controlled delivery of antibiotics in the treatment of osteomyelitis following total joint replacement

Galactooligosaccharides as Potential Prebiotics

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