β-Galactosidase marker genes to tag and track human hematopoietic cells

Bagnis, Claude; Chabannon, Christian; Mannoni, P

doi:10.1038/sj.cgt.7700016

Cited by 8 publications

(2 citation statements)

References 85 publications

(109 reference statements)

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“…It has medical [1], nutritional [2], biotechnological [3,4] and therapeutic [5] importance. Particularly, the b-galactosidase from Escherichia coli was instrumental in the development of the operon model [6], and today is one of the most commonly used enzymes in molecular biology [7].…”

Section: Introductionmentioning

confidence: 99%

Membrane adsorption or penetration differentially modulates β-galactosidase activity against soluble substrates

Sánchez

Perillo

2002

Colloids and Surfaces B: Biointerfaces

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Section: Introductionmentioning

confidence: 99%

Membrane adsorption or penetration differentially modulates β-galactosidase activity against soluble substrates

Sánchez

Perillo

2002

Colloids and Surfaces B: Biointerfaces

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“…Cytosolic markers like ␤-galactosidase (␤-gal) or alkaline phosphatase (AP) have frequently been used to identify gene-engineered cells in vivo, using the appropriate fluorescent or chromogenic substrates (Bagnis et al, 1994(Bagnis et al, , 1999Staal et al, 1995Staal et al, , 1996Fisher-Adams et al, 1996;Chan et al, 1998;Chatterjee et al, 1999). ␤-gal has been widely used in studies examining cell migration and function during embryogenesis (Cui et al, 1994;Spergel et al, 2001).…”

Section: Somatically Gene-transferred Markersmentioning

confidence: 99%

New tools to trace populations of inflammatory cells in the CNS

Flügel

Bradl

2001

Glia

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Cells of the central nervous system (CNS) and immune system communicate regularly. There is a constant surveillance of the intact, healthy CNS by activated T-cells, and massive infiltration of the CNS by immune cells under pathological conditions such as neurodegeneration or neuroinflammation. Labeling CNS-infiltrating T-cells is an essential tool to identify the signals and mechanisms, which mediate the interaction between immune cells and cells of the CNS. In this article, we will present an overview describing currently used cellular markers and demonstrate how these markers have contributed to our current knowledge of CNS inflammation and immune surveillance.

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Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp)

Bartosch

Cosset

Methods in Molecular Biology

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HCV infection leads in 50 to 80% of cases to chronic hepatitis, liver cirrhosis, or hepatocellular carcinoma. Interferons and the nucleoside analog ribavirin form the basis for treatment but are not sufficiently effective and have numerous side effects. Although about 300 million people worldwide are estimated to be infected, the characterization of HCV biology and associated pathologies and development of new therapeutics have been slow. Systems that support HCV replication and particle formation in vitro have emerged only over the last few years, over 15 years after the discovery of the virus. The available infection models have remained limited to chimpanzee (1) and immunodeficient mice carrying engrafted human liver cells (2). HCV pseudoparticles (HCVpp) were the first in vitro infection system to become available for investigation of entry and neutralization of this major human pathogen. HCVpp are formed by incorporation of the full-length hepatitis C virus glycoproteins E1 and E2 onto lenti- or retroviral core particles. HCVpp have been validated by many research groups, closely mimic the functionality of the wild-type virus in terms of cell entry and neutralization, and have even been used to isolate the recent HCV receptor Claudin-1. HCVpp are a useful model system not only because of the functional conservation of the envelope glycoproteins with those of the wild-type virus, but also because the retro- or lentiviral vectors used to form them offer of a number of significant technical advantages.

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β-Galactosidase marker genes to tag and track human hematopoietic cells

Cited by 8 publications

References 85 publications

Membrane adsorption or penetration differentially modulates β-galactosidase activity against soluble substrates

Membrane adsorption or penetration differentially modulates β-galactosidase activity against soluble substrates

New tools to trace populations of inflammatory cells in the CNS

Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp)

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