β-Galactosidase production using glycerol and byproducts: Whey and residual glycerin

Machado, Juliana Ribeiro; Behling, Marina Born; Braga, Anna Rafaela Cavalcante; Kalil, Susana Juliano

doi:10.3109/10242422.2015.1100363

Cited by 8 publications

(6 citation statements)

References 23 publications

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“…The central points of any experimental design provide additional degrees of freedom for error estimation, which increases power when testing for statistical significance of effects [43][44][45][46]. In our work, the central points did not provide any electrospun samples (experiments 17-20) difficulting the statistical analysis over the remaining effective samples.…”

Section: Experimental Designmentioning

confidence: 78%

Development and Characterization of Electrospun Nanostructures Using Polyethylene Oxide: Potential Means for Incorporation of Bioactive Compounds

Ramos

Giaconia

Marco

et al. 2020

Colloids and Interfaces

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The development of processes for stabilization of the properties of bioactive compounds has been studied in recent years, and the use of nanotechnology is among the most discussed routes. The present work addressed the assembly of nanostructures using polyethylene oxide (PEO), the production of core-shell nanofibers (NFs) with bioactive compounds, and the evaluation of their microscopic and physical characteristics. Aqueous solutions of PEO were electrospun by varying different process and solution parameters (PEO and NaCl concentrations, feeding rate, the tip-to-collector distance (TCD), and applied voltage) in order to optimize production of nanostructures. The best condition obtained was evaluated to form core-shell NFs composed by jussara pulp as a source of anthocyanins. To assess the production of NFs with PEO and jussara pulp, feed solutions were prepared in acetate buffer (pH 4.5) with 6% PEO and 10% lyophilized jussara pulp, at a feeding rate of 150 μL·h−1 and TCD of 15 cm using an applied voltage of 10 kV to form core-shell NFs. The results revealed the formation of core-shell NFs with a diameter of 126.5 ± 50.0 nm. The outcomes achieved represent a crucial step in the application of anthocyanins in food systems as pigments, establishing a basis for further research on the incorporation of nanomaterials into foodstuff.

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Section: Experimental Designmentioning

confidence: 78%

Development and Characterization of Electrospun Nanostructures Using Polyethylene Oxide: Potential Means for Incorporation of Bioactive Compounds

Ramos

Giaconia

Marco

et al. 2020

Colloids and Interfaces

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“…(2) shows the equation model fitted according to the regression analysis. Experimental design it is a great statistical tool that uses regression analysis and is widely used in bioprocessing, especially in culture medium optimization (Braga et al, 2012;Machado et al, 2015).…”

Section: − = +mentioning

confidence: 99%

Lactobacillus fermentation of jussara pulp leads to the enzymatic conversion of anthocyanins increasing antioxidant activity

Braga

Mesquita

Martins

et al. 2018

Journal of Food Composition and Analysis

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Bacteria possessing an enzymatic system able to metabolize anthocyanins may play a major role in the production of compounds with different bioavailability and biological activity. In this study, Lactobacillus and Bifidobacterium strains were screened for the enzymatic activities of α-galactosidase, β-galactosidase and βglucosidase. These strains were also screened for their ability to convert the anthocyanins from jussara. The nine evaluated strains produced at least two of the three enzymes; they were also capable of changing the main anthocyanin's chromatographic profile. The values of cyanidin 3-glucoside and cyanidin 3-rutinoside, following fermentation, ranged from 0 to 3217.7 and 4323.3 to 17190.9 μg/100 mL of medium fermented, respectively. The Lactobacillus deubruekii strain was able to change the anthocyanins from jussara pulp more extensively. Therefore, a culture medium containing jussara pulp and glucose was optimized using the experimental design as a statistical tool. Medium maximization occurred with a mixture composing 20% jussara pulp and 10% glucose in which the microorganism was able to reach the highest enzymatic production as well as produce the most extensive conversion of the main anthocyanins. Protocatechuic acid was the main enzymatic bioconversion product identified following fermentation. In addition, there was an observed increase in antioxidant activity following the jussara pulp fermentation process.

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“…Currently, β-gal is mainly obtained by submerged fermentation using Kluyveromyces lactis because the yeast has high enzyme production ability and adapts broadly to the dairy environment (You et al 2017). As in most biotechnological processes, this process involves the recovery of the β-gal using different strategies (Machado et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Recovery of β-galactosidase produced by Kluyveromyces lactis by ion-exchange chromatography: Influence of pH and ionic strength parameters

Carvalho

Júnior

Lima

et al. 2022

An. Acad. Bras. Ciênc.

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The use of β-galactosidase in food products has been a major focus of the industry. Therefore, the development of effi cient and inexpensive methodologies to purify it is essential. Thus, this study aimed to recover the enzyme β-galactosidase (β-gal) by ion-exchange chromatography in a fi xed-bed column. Batch adsorption tests were performed using four types of adsorbents. The β-gal adsorption capacity in batch mode using Streamline DEAE resin presented the best performance, with a retention capacity of 18.77 ± 0.14 U/g at pH 6.0. A 2 2 experimental design was applied to optimize the β-gal recovery using an AKTA Start system, evaluating the ionic strength and the pH as process parameters. The results showed that ionic strength exerted a greater infl uence on fold purifi cation (FP). The β-gal fraction in elution using 0.1-0.4 M of NaCl showed a yield of 51.65 ± 0.17% and FP of 2.00 ± 0.43. Electrophoresis confi rmed the β-gal recovery, where an evident band with a molecular weight between 60 and 120 kDa was observed. These results point to the recovery of a stable β-gal of K. lactis with potential industrial applications.

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β-Galactosidase production using glycerol and byproducts: Whey and residual glycerin

Cited by 8 publications

References 23 publications

Development and Characterization of Electrospun Nanostructures Using Polyethylene Oxide: Potential Means for Incorporation of Bioactive Compounds

Development and Characterization of Electrospun Nanostructures Using Polyethylene Oxide: Potential Means for Incorporation of Bioactive Compounds

Lactobacillus fermentation of jussara pulp leads to the enzymatic conversion of anthocyanins increasing antioxidant activity

Recovery of β-galactosidase produced by Kluyveromyces lactis by ion-exchange chromatography: Influence of pH and ionic strength parameters

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