β-glucan and anisodamine can enhance the immersion immune efficacy of inactivated cyprinid herpesvirus 2 vaccine in Carassius auratus gibelio

Yan, Yiyi; Huo, Xingchen; Ai, Taoshan; Su, Jian

doi:10.1016/j.fsi.2020.01.025

Cited by 38 publications

(18 citation statements)

References 36 publications

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“…Yan et al . (2020) proved the efficiency of β‐propiolactone‐inactivated Cyprinid herpesvirus‐2, mixed with β‐glucan or anisodamine as immersion immune adjuvant and also provided reference for improving the efficiency of immersion immunity. A DNA vaccine, pEGFP‐N1‐ORF25 based on the ORF25 gene of CyHV‐2 was constructed by Yuan et al .…”

Section: Prophylaxis and Controlmentioning

confidence: 95%

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

Thangaraj

Nithianantham

Dharmaratnam

et al. 2020

Reviews in Aquaculture

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Cyprinid herpesvirus-2 (CyHV-2) is a linear double-stranded DNA virus in the genus Cyprinivirus of family Alloherpesviridae. The virus is known to be highly pathogenic to ornamental goldfish (Carassius auratus), crucian carp (C. carassius) and Gibel carp (C. auratus gibelio), and also to the hybrids of goldfish and other carps. Cyprinid herpesvirus-2, having the smallest genome (290.3 kb) among Cyprinivirus, causes herpesviral hematopoietic necrosis disease (HVHND) that results in huge economic losses in aquaculture industry as the disease can cause high mortality (50-100%) among the affected fish. The disease was initially reported as the cause of epizootics in juvenile goldfish of Japan during 1992 and 1993. To date, this disease has been reported around the world including Europe, North America, Oceania and Asia. Huge economic losses due to the CyHV-2 infection among cultured gibel carp in China, during 2011-2012, mass mortality in crucian carp during 2012 in Italy, 95% mortality in goldfish during 2014 in France, 85% mortality in goldfish during 2016 in Poland had been reported. Strategies for controlling the spread of CyHV-2 are thus urgently required to limit economic damage. Furthermore, the review will shed light on lacunae in current knowledge as well as on the perspectives that merits further investigations on CyHV-2 research. The paper forms the first comprehensive overview of CyHV-2 causing a serious economically significant fish disease and, will be helpful for the researchers to get all related information from a single manuscript.

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Section: Prophylaxis and Controlmentioning

confidence: 95%

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

Thangaraj

Nithianantham

Dharmaratnam

et al. 2020

Reviews in Aquaculture

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“…The protection rate is the best reflection of the vaccine efficacy. In our previous study, the immersion immunization with an inactivated vaccine had a 26% protection rate and a 14% increase after the addition of β-glucan [24]. Previous other report showed that the relative survival rates of goldfish immunized with formalin-inactivated CyHV-2 were 42.5% and 57.6%, challenged at 4 and 8 weeks post vaccination, respectively [31].…”

Section: Discussionmentioning

confidence: 76%

“…The inactivated vaccine of CyHV-2 was preserved in our laboratory [24]. Briefly, gibel carp were intraperitoneally injected with 200 µL of virus suspension respectively.…”

Section: Preparation Of Inactivated Vaccine Dna Vaccine and Moleculamentioning

confidence: 99%

The Combination of Molecular Adjuvant CCL35.2 and DNA Vaccine Significantly Enhances the Immune Protection of Carassius auratus gibelio against CyHV-2 Infection

Huo

Fan

et al. 2020

Vaccines

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Cyprinid herpesvirus 2 (CyHV-2) infection results in huge economic losses in gibel carp (Carassius auratus gibelio) industry. In this study, we first constructed recombinant plasmids pcORF25 and pcCCL35.2 as DNA vaccine and molecular adjuvant against CyHV-2, respectively, and confirmed that both recombinant plasmids could be effectively expressed in vitro and in vivo. Then, the vaccination and infection experiments (n = 50) were set as seven groups. The survival rate (70%) in ORF25/CCL35.2 group was highest. The highest specific antibody levels were found in ORF25/CCL35.2 group in major immune tissues by qRT-PCR, and confirmed in serum by ELISA assay, antibody neutralization titer, and serum incubation-infection experiments. Three crucial innate immune indices, namely C3 content, lysozyme, and total superoxide dismutase (TSOD) activities, were highest in ORF25/CCL35.2 group in serum. pcORF25/pcCCL35.2 can effectively up-regulate mRNA expressions of some important immune genes (IL-1β, IL-2, IFN-γ2, and viperin), and significantly suppress CyHV-2 replication in head kidney and spleen tissues. The minimal tissue lesions can be seen in ORF25/CCL35.2 group in gill, spleen, and trunk kidney tissues by histopathological examination. The results indicated that the combination of DNA vaccine pcORF25 and molecular adjuvant pcCCL35.2 is an effective method against CyHV-2 infection, suggesting a feasible strategy for the control of fish viral diseases.

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“…Regarding microbiota-derived components, the immune-stimulating effects of β-glucans on the fish immune system when administered on their own have become evident using different administration routes ( 109 ). Recently, immersion vaccination of gilbel carp ( Carassius auratus gibelio ) with β-glucans and β-propiolactone-inactivated cyprinid herpesvirus 2 protected against a viral challenge more efficiently than fish vaccinated in the absence of β-glucans ( 110 ). In this work, the levels of transcription of IL-2 and IFN-γ2 in head kidney and spleen as well as of IgM and IgZ in the spleen were higher when β-glucans were included ( 110 ).…”

Section: Strategies For Improving the Efficiency Of Mucosal Vaccinatimentioning

confidence: 99%

“…Recently, immersion vaccination of gilbel carp ( Carassius auratus gibelio ) with β-glucans and β-propiolactone-inactivated cyprinid herpesvirus 2 protected against a viral challenge more efficiently than fish vaccinated in the absence of β-glucans ( 110 ). In this work, the levels of transcription of IL-2 and IFN-γ2 in head kidney and spleen as well as of IgM and IgZ in the spleen were higher when β-glucans were included ( 110 ). In rainbow trout, a recombinant flagellin from the salmonid pathogen Y. ruckeri induced a strong antibody response when intraperitoneally injected ( 111 ).…”

Section: Strategies For Improving the Efficiency Of Mucosal Vaccinatimentioning

confidence: 99%

Systemic and Mucosal B and T Cell Responses Upon Mucosal Vaccination of Teleost Fish

Muñoz-Atienza¹,

Díaz‐Rosales²,

Tafalla³

2021

Front. Immunol.

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The development of mucosal vaccines against pathogens is currently a highly explored area of research in both humans and animals. This is due to the fact that mucosal vaccines have the potential to best elicit protective responses at these mucosal surfaces, which represent the frontline of host defense, thus blocking the pathogen at its initial replication sites. However, in order to provide an efficient long-lasting protection, these mucosal vaccines have to be capable of eliciting an adequate systemic immune response in addition to local responses. In aquaculture, the need for mucosal vaccines has further practical implications, as these vaccines would avoid the individual manipulation of fish out of the water, being beneficial from both an economic and animal welfare point of view. However, how B and T cells are organized in teleost fish within these mucosal sites and how they respond to mucosally delivered antigens varies greatly when compared to mammals. For this reason, it is important to establish which mucosally delivered antigens have the capacity to induce strong and long-lasting B and T cell responses. Hence, in this review, we have summarized what is currently known regarding the adaptive immune mechanisms that are induced both locally and systemically in fish after mucosal immunization through different routes of administration including oral and nasal vaccination, anal intubation and immersion vaccination. Finally, based on the data presented, we discuss how mucosal vaccination strategies could be improved to reach significant protection levels in these species.

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β-glucan and anisodamine can enhance the immersion immune efficacy of inactivated cyprinid herpesvirus 2 vaccine in Carassius auratus gibelio

Cited by 38 publications

References 36 publications

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

The Combination of Molecular Adjuvant CCL35.2 and DNA Vaccine Significantly Enhances the Immune Protection of Carassius auratus gibelio against CyHV-2 Infection

Systemic and Mucosal B and T Cell Responses Upon Mucosal Vaccination of Teleost Fish

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