β-glucan attenuates cognitive impairment via the gut-brain axis in diet-induced obese mice

Shi, Hongli; Yu, Yinghua; Lin, Danhong; Zheng, Peng; Zhang, Peng; Mei, Hu; Wang, Qiao; Pan, Wei; Yang, Xiaoying; Hu, Tao; Li, Qianqian; Tang, Renxian; Zhou, Feng; Zheng, Kuiyang; Huang, Xu‐Feng

doi:10.1186/s40168-020-00920-y

Cited by 162 publications

(129 citation statements)

References 67 publications

Supporting

Mentioning

120

Contrasting

Order By: Relevance

“…However, no significant difference was found in other inflammatory cytokines in the ileum and IL-6 and IL-10 level in serum. Although other studies reported that a long-term intake of oat β-glucan lowered the levels of inflammatory cytokines in the colon [ 42 ], we found no convincing evidence of an anti-inflammatory effect. Further studies are needed to investigate the anti-inflammatory effect of HGB flour.…”

Section: Discussioncontrasting

confidence: 99%

Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects

Mio

Otake

Nakashima³

et al. 2021

Nutrients

View full text Add to dashboard Cite

The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated. C57BL/6J male mice were fed a high-fat diet supplemented with HGB flour for 90 days. Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA). Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum. Concentrations of short-chain fatty acids (SCFAs) and organic acids were analyzed by GC/MS. Concentrations of sIgA in the cecum and serum were increased in the HGB group compared to the control. Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group. HGB intake increased the bacterial count of microbiota, such as Bifidobacterium and Lactobacillus. Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the IL-10 expression level. Our findings showed that HGB flour enhanced immune function such as IgA secretion and IL-10 expression, even when the immune system was deteriorated by a high-fat diet. Moreover, we found that HGB flour modulated the gut microbiota, which increased the concentration of SCFAs, thereby stimulating the immune system.

show abstract

Section: Discussioncontrasting

confidence: 99%

Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects

Mio

Otake

Nakashima³

et al. 2021

Nutrients

View full text Add to dashboard Cite

show abstract

“…Additionally, SCFAs also suppress the synthesis of the hunger-suppressing hormones leptin, peptide YY and glucagon-like peptide 1 43 . Consistent with a recent study 35 , propionate-induced activation of AKT was shown to be involved in regulating lipid accumulation by indirectly regulating Acc1 expression 17 , 18 and nutrient absorption-related gene transcription in a human FHC cell line (Figure 6 E). Consistently, the levels of Acc1 were increased in the WAT, liver and intestine (Figures 3 C, 3D and 4B).…”

Section: Discussionsupporting

confidence: 91%

“…Genomic DNA amplification and sequencing were performed as described in a previous study 17 . Briefly, total DNA was extracted from feces ( n = 3-5/group) using an E.Z.N.A.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Aurka deficiency in the intestinal epithelium promotes age-induced obesity via propionate-mediated AKT activation

Sun¹,

Meng²,

Zhao³

et al. 2021

Int. J. Biol. Sci.

Self Cite

View full text Add to dashboard Cite

Aurora-A kinase, a serine/threonine mitotic kinase involved in mitosis, is overexpressed in several human cancers. A recent study showed that Aurora-A mediates glucose metabolism via SOX8/FOXK1 in ovarian cancer. However, the roles of Aurora-A in metabolic diseases remain unclear. This study found that Aurka loss in the intestinal epithelium promoted age-induced obesity and enlargement of lipid droplets in parallel with an increase in infiltrated macrophages in the white adipocyte tissue (WAT) of male mice. Moreover, loss of Aurka induced the expression of lipid metabolism regulatory genes, including acetyl-coenzyme A carboxylase 1 ( Acc1 ), in association with an increase in the levels of p-AKT in the intestinal epithelium as well as WAT. Blockade of AKT activation reduced the expression of lipid metabolism regulatory genes. In subsequent experiments, we found that the Firmicutes abundance and the levels of short-chain fatty acids (SCFAs) in the gut were dramatically increased in Aurka f/+ ;Villin Cre/+ mice compared with Aurka f/+ mice. Additionally, propionate increased the phosphorylation of AKT in vitro . These observations indicated that Aurka loss in the intestinal epithelium contributed to gut microbiota dysbiosis and higher levels of SCFAs, especially propionate, leading to AKT activation and lipid metabolism regulatory gene expression, which in turn promoted age-induced obesity.

show abstract

“…Arabinoxylan plays an important role in maintaining the integrity of the gut by increasing the proliferation of goblet cells as well as secretion of IgA. β -glucan not only could improve metabolic condition, but also could attenuate cognitive impairment [ 7 , 8 , 9 ]. Glucomannan is helpful for bone metabolism and immunity [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Fermentation Characteristics and Fiber-Degrading Enzyme Kinetics of Cellulose, Arabinoxylan, β-Glucan and Glucomannan by Pig Fecal Microbiota

Bai

Zhou

et al. 2021

Microorganisms

View full text Add to dashboard Cite

Non-digestible polysaccharides are of great significance to human and animal intestinal health. Cellulose, arabinoxylan, β−glucan and glucomannan were selected in the present study to investigate the fermentation characteristics and fiber-degrading enzyme kinetics by inoculating pig fecal microbiota in vitro. Our results showed that fermentation of arabinoxylan and β-glucan produced the highest amount of acetate and lactate, respectively. The abundance of Prevotella_9 was the highest in β-glucan group and positively correlated with lactate and acetate. Glucomannan fermentation produced the highest amount of butyrate, and the abundance of Lachnospiraceae_XPB_1014_group and Bacteroides were the lowest. A significant negative correlation was found between Lachnospiraceae_XPB_1014_group, Bacteroides and butyrate. Exo-β-1,4-xylanase had the highest activity at 24 h during arabinoxylan fermentation. The activity of β-glucosidase and β-mannosidase at 36 h were higher than those at 15 h in the glucomannan group. The abundance of Prevotella_9 was positively correlated with β-glucosidase while Lachnospiraceae_XPB_1014_group and Bacteroides were negatively correlated with β-xylosidase. Our findings demonstrated the β-glucan and arabinoxylan promote proliferation of Prevotella_9, with the preference to secret β-glucosidase, β-mannosidase and the potential to produce lactate and acetate. Butyrate production can be improved by inhibiting the proliferation of Lachnospiraceae_XPB_1014_group and Bacteroides, which have the lack of potential to secret β-xylosidase.

show abstract

β-glucan attenuates cognitive impairment via the gut-brain axis in diet-induced obese mice

Cited by 162 publications

References 67 publications

Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects

Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects

Aurka deficiency in the intestinal epithelium promotes age-induced obesity via propionate-mediated AKT activation

In Vitro Fermentation Characteristics and Fiber-Degrading Enzyme Kinetics of Cellulose, Arabinoxylan, β-Glucan and Glucomannan by Pig Fecal Microbiota

Contact Info

Product

Resources

About