β-Glucan-Induced Trained Immunity in Dogs

Paris, Simon; Chapat, Ludivine; Pasin, Marion; Lambiel, Manon; Sharrock, Thomas E; Shukla, Rishabh; Sigoillot-Claude, Cécile; Bonnet, Jeanne‐Marie; Poulet, Hervé; Freyburger, Ludovic; Luca, Karelle De

doi:10.3389/fimmu.2020.566893

Cited by 10 publications

(7 citation statements)

References 44 publications

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“…Trained immunity is a non-specific immune response arising from vaccination or infection that enhances the responsiveness of immune cells such as monocytes, macrophages, and natural killer (NK) cells to further heterologous infections [ 9 , 10 ]. This phenomenon occurs in the hematopoietic stem cells of the bone marrow, and the effects can be seen in the myeloid progenitors [ 9 , 11 , 12 ]. The trained phenotype observed in immune and endothelial cells has been attributed to epigenetic modifications [ 9 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

BCG-Based Vaccines Elicit Antigen-Specific Adaptive and Trained Immunity against SARS-CoV-2 and Andes orthohantavirus

et al. 2022

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Background:Mycobacterium bovis Bacillus Calmette-Guérin (BCG) is a live attenuated vaccine mainly administered to newborns and used for over 100 years to prevent the disease caused by Mycobacterium tuberculosis (M. tb). This vaccine can induce immune response polarization towards a Th1 profile, which is desired for counteracting M. tb, other mycobacteria, and unrelated intracellular pathogens. The vaccine BCG has been used as a vector to express recombinant proteins and has been shown to protect against several diseases, particularly respiratory viruses. Methods: BCG was used to develop recombinant vaccines expressing either the Nucleoprotein from SARS-CoV-2 or Andes orthohantavirus. Mice were immunized with these vaccines with the aim of evaluating the safety and immunogenicity parameters. Results: Immunization with two doses of 1 × 108 CFU or one dose of 1 × 105 CFU of these BCGs was safe in mice. A statistically significant cellular immune response was induced by both formulations, characterized as the activation of CD4+ and CD8+ T cells. Stimulation with unrelated antigens resulted in increased expression of activation markers by T cells and secretion of IL-2 and IFN-γ, while increased secretion of IL-6 was found for both recombinant vaccines; all of these parameters related to a trained immunity profile. The humoral immune response elicited by both vaccines was modest, but further exposure to antigens could increase this response. Conclusions: The BCG vaccine is a promising platform for developing vaccines against different pathogens, inducing a marked antigen-specific immune response.

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Section: Introductionmentioning

confidence: 99%

BCG-Based Vaccines Elicit Antigen-Specific Adaptive and Trained Immunity against SARS-CoV-2 and Andes orthohantavirus

et al. 2022

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“…Trained immunity is an evolutionarily conservative innate immune mechanism that exists in multiple vertebrate species including mammals [ 26 ], chickens [ 28 ], and fishes [ 42 ]. This study showed that exposure to C. perfringens increases the production of NO in primed PBMC compared to non-primed.…”

Section: Discussionmentioning

confidence: 99%

Extracellular trap can be trained as a memory response

et al. 2022

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Extracellular trap (ET) appears as a double-edged sword for the host since it participates in host immune defense by entrapping pathogens, while excessive ET release also contributes to various diseases progression including atherosclerosis, cancer, and autoimmune disorders. A better understanding of ET formation and regulation will be beneficial for developing strategies for infection control and ET-associated disease treatment. There is some evidence indicating that prior infection can enhance extracellular killing. Neutrophils from cancer or sepsis are predisposed to generate ET. It is reasonable to suspect that ET may be trained to form as a memory response, just like cytokine memory response termed “trained immunity.” The mice were intraperitoneally injected with heat-killed Candida albicans (HK- C. albicans ), 3 days later bone marrow-derived macrophages (BMDM) were isolated and challenged with Clostridium perfringens as a second stimulation. We found that HK- C. albicans priming enhanced ET formation upon Clostridium perfringens infection, accompanied by increased extracellular killing capacity. Mannan priming also enhanced ET formation. Since ETs memory was induced in chicken PBMC, ETs memory may be evolutionarily conserved. Moreover, mTOR was required for ETs memory response. Collectively, this study showed that ETs can be trained as a memory response and indicated that memory property of ETs should be considered during the understanding of recurrent infection and ET-associated disorders.

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“…Recognition of b-glucans by Dectin-1 has been shown to induce epigenetic modifications in immune cells, that render them more efficient in responding to infection (66), a phenomenon referred to as trained immunity (66,67). It is therefore conceivable that bovine monocytes, such as those of mice (68), humans (28), dogs (69), and chicken (70) may be prone to induction of trained immunity. That would provide a plausible explanation for the beneficial effects of b-glucancontaining dietary supplements observed in vivo in cattle (23,(45)(46)(47).…”

Section: Discussionmentioning

confidence: 99%

Dectin-1-Mediated Production of Pro-Inflammatory Cytokines Induced by Yeast β-Glucans in Bovine Monocytes

et al. 2021

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Yeast-derived products containing β-glucans have long been used as feed supplements in domesticated animals in an attempt to increase immunity. β-glucans are mainly recognized by the cell surface receptor CLEC7A, also designated Dectin-1. Although the immune mechanisms elicited through Dectin-1 activation have been studied in detail in mice and humans, they are poorly understood in other species. Here, we evaluated the response of bovine monocytes to soluble and particulate purified β-glucans, and also to Zymosan. Our results show that particulate, but not soluble β-glucans, can upregulate the surface expression of costimulatory molecules CD80 and CD86 on bovine monocytes. In addition, stimulated cells increased production of IL-8 and of TNF, IL1B, and IL6 mRNA expression, in a dose-dependent manner, which correlated positively with CLEC7A gene expression. Production of IL-8 and TNF expression decreased significantly after CLEC7A knockdown using two different pairs of siRNAs. Overall, we demonstrated here that bovine monocytes respond to particulate β-glucans, through Dectin-1, by increasing the expression of pro-inflammatory cytokines. Our data support further studies in cattle on the induction of trained immunity using dietary β-glucans.

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β-Glucan-Induced Trained Immunity in Dogs

Cited by 10 publications

References 44 publications

BCG-Based Vaccines Elicit Antigen-Specific Adaptive and Trained Immunity against SARS-CoV-2 and Andes orthohantavirus

BCG-Based Vaccines Elicit Antigen-Specific Adaptive and Trained Immunity against SARS-CoV-2 and Andes orthohantavirus

Extracellular trap can be trained as a memory response

Dectin-1-Mediated Production of Pro-Inflammatory Cytokines Induced by Yeast β-Glucans in Bovine Monocytes

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