β-Lactamase induction and aminoglycoside susceptibility in Pseudomonas aeruginosa

Abstract

Recent clinical reports have described the emergence of cross-resistance to aminoglycosides during beta-lactam therapy. We therefore studied the effect of imipenem-mediated beta-lactamase induction on the susceptibilities of 92 clinical isolates of Pseudomonas aeruginosa to gentamicin, tobramycin, and amikacin. We performed a disc approximation test to look for truncation by imipenem of the inhibitory zone sizes of the aminoglycosides. We also compared aminoglycoside inhibitory zone sizes of strains grown in the presence and absence of subinhibitory concentrations of imipenem. Finally, we compared aminoglycoside MIC values determined with and without subinhibitory concentrations of imipenem. The results showed that simultaneous emergence of resistance to beta-lactams and aminoglycosides is very rare.

Effects of Controlled Cortical Impact on the Mouse Brain Vasculome

“…Stroke GWAS gene suppressor of cytokine signaling 3 (SOCS3) is an inducible negative regulator of signaling by the Janus kinase/ signal transduction and activator of transcription 3 pathway, and it is activated in many CNS injury conditions, [117][118][119] as part of an endogenous response to regulate endothelial cell death, 120,121 pathological angiogenesis, and inflammation response. [122][123][124][125] Together with CD93 and Apelin, SOCS3 is down-regulated in the brain after water deprivation. 126 At the same time, SOCS3 can also act as an intrinsic inhibitor for neural axon regeneration.…”

Pseudomonas Aeruginosa AmpR Transcriptional Regulatory Network

“…The same trend is also seen with the aminoglycoside, tobramycin for P. aeruginosa. The clinical isolates started displaying resistance (<2 µg/ml in 1980, 8 µg/ml in 1987 and ≥ 16 µg/ml in 2000) in a very short time span (51)(52)(53).…”