β‐Lactamase production and antimicrobial susceptibility of subgingival bacteria from refractory periodontitis

Handal, Trude; Olsen, Ingar; Walker, Clay; Caugant, Dominique A.

doi:10.1111/j.1399-302x.2004.00159.x

Cited by 62 publications

(50 citation statements)

References 33 publications

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“…In this study, the detection of ␤-lactamase was limited to Prevotella strains (Table 1). Other studies have revealed that ␤-lactamase-positive strains of facultative anaerobic bacteria such as staphylococci and non-Prevotella anaerobes are also isolated from dental infections (4,7,16). However, even in these previous studies, the incidence of such bacteria and the prevalence of ␤-lactamase-positive strains in these species were very low.…”

Section: Discussioncontrasting

confidence: 41%

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Detection of cfxA and cfxA2 , the β-Lactamase Genes of Prevotella spp., in Clinical Samples from Dentoalveolar Infection by Real-Time PCR

et al. 2006

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While most bacteria involved in dentoalveolar infection are highly susceptible to penicillin, some Prevotella strains exhibit resistance to this agent through the production of ␤-lactamase. The production of ␤-lactamase by Prevotella spp. is in turn associated with the expression of the genes cfxA and cfxA2. The aim of the present study was to determine the prevalence of cfxA and cfxA2 in Prevotella strains by use of real-time PCR and to assess the performance of this molecular method for the direct detection of the genes in 87 clinical samples (pus and root canal exudates) from dentoalveolar infection. Production of ␤-lactamase by each isolate was determined using a nitrocefin disk. ␤-Lactamase production was seen in 31% of Prevotella isolates, while all isolates of other species were ␤-lactamase negative. The penicillin resistance of isolates strongly correlated with the production of ␤-lactamase. Real-time PCR was found to detect the cfxA and cfxA2 genes from at least five cells per reaction mixture (5 ؋ 10 3 CFU/ml of pus). Using real-time PCR, the presence of cfxA and cfxA2 was evident for all 48 ␤-lactamase-positive Prevotella strains. In contrast, neither ␤-lactamase-negative Prevotella (n ‫؍‬ 91) or non-Prevotella (n ‫؍‬ 31) strains were positive for the genes. In this study, 31 of the 87 samples yielded ␤-lactamase-positive Prevotella results, and cfxA and cfxA2 were detected in all 31 samples. Of the 56 culture-negative samples, 8 (14%) were positive for cfxA and cfxA2 by the real-time PCR. This sensitive and specific molecular method offers a rapid clinical test for aiding in the selection of an appropriate antibiotic for treatment of dentoalveolar infection. Although penicillin remains largely effective in the treatment of dentoalveolar infection, ␤-lactamase-stable antibiotics should be considered in cases in which ␤-lactamase-positive Prevotella strains are involved.

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Section: Discussioncontrasting

confidence: 41%

“…Since Prevotella spp. are also implicated in other oral and nonoral infections, such as periodontitis, osteomyelitis, sinusitis, and sepsis (4,7,23), this molecular diagnostic method may also prove of value in aiding treatment of these infections.…”

Section: Discussionmentioning

confidence: 99%

Detection of cfxA and cfxA2 , the β-Lactamase Genes of Prevotella spp., in Clinical Samples from Dentoalveolar Infection by Real-Time PCR

et al. 2006

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“…In the present investigation, some isolates that are resistant to b-lactams were not to be producers of ß-lactamase by the methodology employed in this study (Table II), suggesting suggests that these microorganisms are producers of non-exportable ß-lactamases, as previously reported in Gram-negative bacteria (Handal et al, 2004;Gaetti-Jardim Júnior et al, 2007).…”

Section: Discussionsupporting

confidence: 70%

Antimicrobial Susceptibility to ß-lactams and Metronidazole of Microorganisms Isolated from Chronic and Aggressive Periodontitis

Júnior

Schweitzer

2013

Int. J. Odontostomat.

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susceptibility to ß-lactams and metronidazole of microorganisms isolated from chronic and aggressive periodontitis. Int. J. Odontostomat., 7(2):193-198, 2013. ABSTRACT:The objective of this study was to evaluate the microbial susceptibility to ß-lactams and metronidazole, and evaluate the production of ß-lactamases by microorganisms isolated from patients with chronic or aggressive periodontitis. The samples were obtained from 50 patients with periodontitis and microorganisms were isolated onto selective and nonselective culture media, identified by biochemical methods and tested for susceptibility to antimicrobial agents (amoxicillin, amoxicillin/clavulanate, cefoxitin, imipenem, metronidazole, penicillin G). The isolates were resistant to at least 1 mg/ml of any drug tested were evaluated to verify the production of ß-lactamases by the method of double layer (or biological) and chromogenic cephalosporin using nitrocefin. The results evidenced resistance to amoxicillin and penicillin G, while the susceptibility to association amoxicillin/clavulanate, imipenem and cefoxitin was widely disseminated among the organisms. Resistance to these drugs showed a clear correlation with the production of ß-lactamase in the majority of microbial groups.

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“…Slots (14), Listgarten (23) y Handal (24), encontraron en diferentes estudios, que en pacientes tratados con terapia mecánica tradicional no había mejoría en aquellas bolsas periodontales que presentaban enterobacterias.…”

Section: Introductionunclassified

Efecto de las enterobacterias en pacientes con periodontitis crónica

Medina

2010

Avances en Periodoncia

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Efecto de las enterobacterias en pacientes con periodontitis crónicaEffect of enterobacteriaceae in patients with chronic periodontitis ARDILA MEDINA CM* RESUMENLa periodontitis crónica es una enfermedad infecciosa asociada a microorganismos gram-negativos anaerobios. Esta entidad de naturaleza crónica y de alta prevalencia en la población, ha sido relacionada con un riesgo aumentado a infarto agudo del miocardio, accidente cerebrovascular, bajo peso al nacer y parto pretérmino. Estudios microbiológicos en pacientes con periodontitis en diferentes partes del mundo han mostrado altas prevalencias de sobreinfección por enterobacterias, las cuales podrían complicar el cuadro clínico de los pacientes y la respuesta a la terapia periodontal. Así mismo, el lipopolisacári-do presente en su pared, ha demostrado estimular la activación celular y la producción de citoquinas proinflamatorias que podrían aumentar el riesgo de enfermedades sistémicas en estos pacientes. Aunque la terapia periodontal reduce los marcadores inflamatorios en suero y ha mostrado una reducción en la incidencia de bajo peso al nacer y parto pretérmino, no se ha estudiado el efecto de las enterobacterias a nivel subgingival sobre la respuesta a la terapia básica y los efectos sisté-micos que pueda desencadenar. El objetivo de esta revisión es proporcionar fundamentos que permitan de alguna manera dilucidar tal problemática. PALABRAS CLAVE:Periodontitis, microbiología, enterobacterias, patógenos. SUMMARYThe chronic periodontitis is an infectious disease associated to anaerobic gram negative microorganisms. This organization of chronic nature and high prevalence in the population has been associated with a risk increased to acute infarct of the myocardium, stroke, low birth weight and preterm infants. Microbiological studies in patients with periodontitis in different parts from the world have shown high prevalences of sobreinfection by enterobacteriaceae, which could complicate the clinical picture of the patients and the answer to the periodontal therapy. Also, the present lipopolisacaric one in its wall, has demonstrated to stimulate the cellular activation and the production of proinflammatory cytokines that could increase the risk of systemic diseases in these patients. Although the periodontal therapy reduces the inflammatory serum markers and has shown a reduction in the incidence of low birth weight and preterm infants, has not studied the effect of enterobacteriaceae at subgingival level on the sistemic answer to the basic therapy and effects that can trigger. The objective of this revision is to provide foundations that somehow allow to explain so problematic.

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β‐Lactamase production and antimicrobial susceptibility of subgingival bacteria from refractory periodontitis

Cited by 62 publications

References 33 publications

Detection of cfxA and cfxA2 , the β-Lactamase Genes of Prevotella spp., in Clinical Samples from Dentoalveolar Infection by Real-Time PCR

Detection of cfxA and cfxA2 , the β-Lactamase Genes of Prevotella spp., in Clinical Samples from Dentoalveolar Infection by Real-Time PCR

Antimicrobial Susceptibility to ß-lactams and Metronidazole of Microorganisms Isolated from Chronic and Aggressive Periodontitis

Efecto de las enterobacterias en pacientes con periodontitis crónica

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