β-lactams resistance gene quantification in an antibiotic resistant Escherichia coli water suspension treated by advanced oxidation with UV/H2O2

Ferro, Giovanna; Guarino, Francesco; Cicatelli, Angela; Rizzo, Luigi

doi:10.1016/j.jhazmat.2016.03.014

Cited by 106 publications

(39 citation statements)

References 45 publications

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“…The disinfection treatment did not affect the relative proportion of ermB over time ( Figure 2D). This is in agreement with the fact that WWTPs are not specifically designed to remove ARGs (Zhang et al, 2015) and with recent experimental results obtained by testing the effect of advanced oxidation process on β-lactams resistance gene, showing that the tested ARG was unaffected by the disinfection treatment (Ferro et al, 2017).…”

Section: Discussionsupporting

confidence: 91%

Defence strategies and antibiotic resistance gene abundance in enterococci under stress by exposure to low doses of peracetic acid

et al. 2017

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Peracetic acid (PAA) is an organic compound used efficiently as disinfectant in wastewater treatments. Yet, at low doses it may cause selection; thus, the effect of low doses of PAA on Enterococcus faecium as a proxy of human-related microbial waste was evaluated. Bacteria were treated with increasing doses of PAA (from 0 to 25 mg L min) and incubated in regrowth experiments under non-growing, limiting conditions and under growing, favorable conditions. The changes in bacterial abundance, in bacterial phenotype (number and composition of small cell clusters), and in the abundance of an antibiotic resistance gene (ARG) was evaluated. The experiment demonstrated that the selected doses of PAA efficiently removed enterococci, and induced a long-lasting effect after PAA inactivation. The relative abundance of small clusters increased during the experiment when compared with that of the inoculum. Moreover, under growing favorable conditions the relative abundance of small clusters decreased and the number of cells per cluster increased with increasing PAA doses. A strong stability of the measured ARG was found, not showing any effect during the whole experiment. The results demonstrated the feasibility of low doses of PAA to inactivate bacteria. However, the stress induced by PAA disinfection promoted a bacterial adaptation, even if potentially without affecting the abundance of the ARG.

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Section: Discussionsupporting

confidence: 91%

Defence strategies and antibiotic resistance gene abundance in enterococci under stress by exposure to low doses of peracetic acid

et al. 2017

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“…The evaluation of the effect of a UV-driven oxidation induced by H 2 O 2 ([H 2 O 2 ] ¼ 20 mg L À1 ) on the potential of antibiotic resistance transfer through the inactivation of an antibiotic-resistant E. coli strain (ampicillin, ciprofloxacin, and tetracycline) and bla TEM (DNA extract from an E. coli strain) has been recently examined by Ferro et al (2017). Whilst the detection limit of antibiotic-resistant E. coli was reached after 240 min, this treatment duration seemed to be insufficient in removing bla TEM , the latter being still present even if the treatment time was extended to 300 min.…”

Section: Ozonationmentioning

confidence: 99%

The role of operating parameters and oxidative damage mechanisms of advanced chemical oxidation processes in the combat against antibiotic-resistant bacteria and resistance genes present in urban wastewater

2018

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An upsurge in the study of antibiotic resistance in the environment has been observed in the last decade. Nowadays, it is becoming increasingly clear that urban wastewater is a key source of antibiotic resistance determinants, i.e. antibiotic-resistant bacteria and antibiotic resistance genes (ARB&ARGs). Urban wastewater reuse has arisen as an important component of water resources management in the European Union and worldwide to address prolonged water scarcity issues. Especially, biological wastewater treatment processes (i.e. conventional activated sludge), which are widely applied in urban wastewater treatment plants, have been shown to provide an ideal environment for the evolution and spread of antibiotic resistance. The ability of advanced chemical oxidation processes (AOPs), e.g. light-driven oxidation in the presence of HO, ozonation, homogeneous and heterogeneous photocatalysis, to inactivate ARB and remove ARGs in wastewater effluents has not been yet evaluated through a systematic and integrated approach. Consequently, this review seeks to provide an extensive and critical appraisal on the assessment of the efficiency of these processes in inactivating ARB and removing ARGs in wastewater effluents, based on recent available scientific literature. It tries to elucidate how the key operating conditions may affect the process efficiency, while pinpointing potential areas for further research and major knowledge gaps which need to be addressed. Also, this review aims at shedding light on the main oxidative damage pathways involved in the inactivation of ARB and removal of ARGs by these processes. In general, the lack and/or heterogeneity of the available scientific data, as well as the different methodological approaches applied in the various studies, make difficult the accurate evaluation of the efficiency of the processes applied. Besides the operating conditions, the variable behavior observed by the various examined genetic constituents of the microbial community, may be directed by the process distinct oxidative damage mechanisms in place during the application of each treatment technology. For example, it was shown in various studies that the majority of cellular damage by advanced chemical oxidation may be on cell wall and membrane structures of the targeted bacteria, leaving the internal components of the cells relatively intact/able to repair damage. As a result, further in-depth mechanistic studies are required, to establish the optimum operating conditions under which oxidative mechanisms target internal cell components such as genetic material and ribosomal structures more intensively, thus conferring permanent damage and/or death and preventing potential post-treatment re-growth.

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“…In another study that used UV fluence of up to 249.5 mJ/cm 2 on secondary-treated municipal wastewater effluent samples, only 0.58-log removal of tetX gene was observed, with a less effective removal (at 0.36-0.4-log) of sul1, tetG and intl1 genes (Zhang et al 2015). Yet another study that assessed UV/H2O2 advanced oxidation processes for disinfection of sterile water spiked with blaTEM gene-carrying E. coli found that the treatment could inactivate the tested antibiotic-resistant E. coli strain, but did not significantly change the copy number per mL of blaTEM gene (Ferro et al 2017). All these studies were in contrast with a study that assessed the effect of UV on secondary-treated municipal wastewater effluent samples and surprisingly found 3-and 1.9-log reduction of erythromycin and tetracycline resistance genes, respectively, by the time 5 mJ/cm 2 fluence is reached .…”

Section: Solar Inactivationmentioning

confidence: 99%

Potential Dissemination of ARB and ARGs into Soil Through the Use of Treated Wastewater for Agricultural Irrigation: Is It a True Cause for Concern?

Aljassim

Hong

2017

Soil Biology

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Resistance to antibiotics is increasingly being recognized as an emerging contaminant posing great risks to effective treatment of infections and to public health. Pristine soils or even soils that predate the antibiotic era naturally contain ARB and ARG. This book chapter explores the native resistome of soils, and collates information on whether soil perturbation through wastewater reuse can lead to accumulation of ARB and ARGs in agricultural soils. Special emphasis was given to ARGs, particularly the blaNDM gene that confers resistance against carbapenem. The fate and persistence of these emerging ARGs have not been studied in depth, however this book chapter reviews available information on other ARGs to gain insight into the possibility of horizontal gene transfer events in wastewater-irrigated soils and plant surfaces and tissues. Lastly, this book chapter visits solar irradiation and bacteriophage treatment as intervention options to limit dissemination of emerging contaminant threats.

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β-lactams resistance gene quantification in an antibiotic resistant Escherichia coli water suspension treated by advanced oxidation with UV/H2O2

Cited by 106 publications

References 45 publications

Defence strategies and antibiotic resistance gene abundance in enterococci under stress by exposure to low doses of peracetic acid

Defence strategies and antibiotic resistance gene abundance in enterococci under stress by exposure to low doses of peracetic acid

The role of operating parameters and oxidative damage mechanisms of advanced chemical oxidation processes in the combat against antibiotic-resistant bacteria and resistance genes present in urban wastewater

Potential Dissemination of ARB and ARGs into Soil Through the Use of Treated Wastewater for Agricultural Irrigation: Is It a True Cause for Concern?

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