β-Lactoglobulin–chlorogenic acid conjugate-based nanoparticles for delivery of (−)-epigallocatechin-3-gallate

Fan, Yuting; Zhang, Yuzhu; Yokoyama, Wallace; Jiang, Yi

doi:10.1039/c6ra28462k

Cited by 46 publications

(21 citation statements)

References 46 publications

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“…The native zein secondary structure composition was 49.65% α-helix, 12.62% β-sheet, 17.78% β-turn, and 20.05% random coil. Dccurrence of secondary structures changes has been found after glycosylation and α-helix structure was strikingly decreased corresponding to the increase of unordered structure, consistent with the observation forβ-Lactoglobulin-chlorogenic acid conjugates (Fan et al, 2017). α-helix is usually buried in the interior site of polypeptide chains (Xue et al, 2013).…”

Section: Characteristics Of the Glycosylated Hydrolysatesupporting

confidence: 84%

Preparation of glycosylated hydrolysate by liquid fermentation with Cordyceps militaris and characterization of its functional properties

Yang¹,

Zheng²,

Li³

et al. 2020

Food Sci. Technol

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This study aimed to investigate the potential of a novel preparation strategy for glycosylated hydrolysate by liquid fermentation in order to provide a high-quality glycosylated hydrolysate. Protein solubility, surface hydrophobicity and antioxidant activity were evaluated. The results of Fourier transform infrared spectra demonstrated the occurrence of glycosylation reaction. The results of Fluorescence emission spectroscope revealed the decrease of tertiary conformation stability. Circular dichroism spectra revealed significant decrease in α-helix and increase in β-sheet and random coil by glycosylation reaction. On comparison with zein hydrolysate, glycosylated hydrolysate had lower surface hydrophobicity but higher in solubility and in vitro digestibility due to glycosylation. Glycosylated hydrolysate presented higher ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) and superoxide radicals scavenging activities as well as Cu 2+ chelating capacity. Ot is concluded that the glycosylation confer an open structure and modified properties of zein, and glycosylated hydrolysate is a potential ingredient with better hydration and antioxidant activities than zein hydrolysate.

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Section: Characteristics Of the Glycosylated Hydrolysatesupporting

confidence: 84%

Preparation of glycosylated hydrolysate by liquid fermentation with Cordyceps militaris and characterization of its functional properties

Yang¹,

Zheng²,

Li³

et al. 2020

Food Sci. Technol

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“…2C, displaying similar spectra in terms of CD intensity and shape. Moreover, the scans exhibited a negative dichroic peak with a minimum at 217 nm (i.e., in the 215 nm region), indicating that the secondary structure of both purified and commercial β-Lg are rich in β-sheet (Estévez et al, 2017;Fan, Zhang, Yokoyama, & Yi, 2017;Yi, Lam, Yokoyama, Cheng, & Zhong, 2014).…”

Section: Independent Variables Levelsmentioning

confidence: 99%

Design of β-lactoglobulin micro- and nanostructures by controlling gelation through physical variables

et al. 2020

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A B S T R A C Tβ-lactoglobulin (β-Lg) is the major protein fraction of bovine whey serum and its principal gelling agent. Its gelation capacity enables conformational changes associated with protein-protein interactions that allow the design of structures with different properties and morphologies. Thus, the aim of this work was to successfully use β-Lg, purified from a commercial whey protein isolate, to develop food-grade micro-(with diameters between 200 and 300 nm) and nano-(with diameters ≤ 100 nm) structures. For this purpose, the phenomena involved in β-Lg gelation were studied under combined effects of concentrations (from 5 to 15 mg mL −1 ), heating temperature (from 60 to 80°C) and heating time (from 5 to 25 min) for pH values of 3, 4, 6 and 7. The effects of such conditions on β-Lg structures were evaluated and the protein was fully characterized in terms of size, polydispersity index (PDI) and surface charge (by dynamic light scattering -DLS), morphology (by transmission electron microscopy -TEM) and conformational structure (circular dichroism, intrinsic and extrinsic fluorescence). Results have shown that β-Lg nanostructures were formed at pH 3 (with diameters between 12.1 and 22.3 nm) and at 7 (with diameters between 8.9 and 35.3 nm). At pH 4 structures were obtained at macroscale (i.e., ≥ 6 μm) for all β-Lg concentrations when heated at 70 and 80°C, independent of the time of heating. For pH 6, it was possible to obtain β-Lg structures either at micro-(245.0 -266.4 nm) or nanoscale (≤ 100 nm) with the lowest polydispersity (PDI) values (≤ 0.25), in accordance with TEM analyses, for heating at 80°C for 15 min. Intrinsic and extrinsic fluorescence data and far-UV circular dichroism spectra measurements revealed conformational changes on β-Lg structure that support these evidences. A strict control of the physical and environmental conditions is crucial for developing β-Lg structures with the desired characteristics, thus calling for the understanding of the mechanisms of protein aggregation and intermolecular interaction when designing β-Lg structures with novel functionalities.

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“…Fan et al [ 58 ] produced β -lactoglobulin-chlorogenic acid nanocarriers for EGCG encapsulation. These nanocarriers could effectively increase EGCG's chemical stability in physiological environments and induce controlled release of EGCG in simulated gastric and intestinal environments, being able to protect EGCG from degradation.…”

Section: Nanocarriers Used To Deliver Egcgmentioning

confidence: 99%

Therapeutic Potential of Epigallocatechin Gallate Nanodelivery Systems

Granja

Frias

Neves

et al. 2017

BioMed Research International

134

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Nowadays, the society is facing a large health problem with the rising of new diseases, including cancer, heart diseases, diabetes, neurodegenerative diseases, and obesity. Thus, it is important to invest in substances that enhance the health of the population. In this context, epigallocatechin gallate (EGCG) is a flavonoid found in many plants, especially in tea. Several studies support the notion that EGCG has several benefits in fighting cancer, heart diseases, diabetes, and obesity, among others. Nevertheless, the poor intestinal absorbance and instability of EGCG constitute the main drawback to use this molecule in prevention and therapy. The encapsulation of EGCG in nanocarriers leads to its enhanced stability and higher therapeutic effects. A comprehensive review of studies currently available on the encapsulation of EGCG by means of nanocarriers will be addressed.

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β-Lactoglobulin–chlorogenic acid conjugate-based nanoparticles for delivery of (−)-epigallocatechin-3-gallate

Abstract: The release of EGCG was controlled by BLG–CA conjugate.

Cited by 46 publications

References 46 publications

Preparation of glycosylated hydrolysate by liquid fermentation with Cordyceps militaris and characterization of its functional properties

Preparation of glycosylated hydrolysate by liquid fermentation with Cordyceps militaris and characterization of its functional properties

Design of β-lactoglobulin micro- and nanostructures by controlling gelation through physical variables

Therapeutic Potential of Epigallocatechin Gallate Nanodelivery Systems

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