β-Laetam Antibiotics Produced by Genetically Engineered Filamentous Fungs

Vichitsoonthonkul, Taweerat; Yw, Chu; Hs, Sodhi; Saunders, Geoffrey Lance

doi:10.1201/9781003067191-9

Recombinant Microbes for Industrial and Agricultural Applications 2020

DOI: 10.1201/9781003067191-9

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β-Laetam Antibiotics Produced by Genetically Engineered Filamentous Fungs

Taweerat Vichitsoonthonkul¹,

Chu Yw²,

Sodhi Hs³

et al.

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Detection of a protein which binds specifically to the upstream region of the pcbAB gene in Penicillium chrysogenum

Chu

Renno²,

Saunders

1995

Curr Genet

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The upstream region of the pcbAB gene from Penicillium chrysogenum was screened for protein-binding sites using an electromobility shift assay. A specific protein/DNA interaction was detected within a fragment covering the region -387 to -242 relative to the pcbAB translational start codon. The appearance of this protein and pcbAB mRNA in culture extracts occurred at the same time point in fermentations, suggesting that the protein might be a transcription activator. The putative upstream activating sequence was located more precisely using cross-competition assays. These indicated the involvement of the 7-bp motif TGCCAAG in the binding of the protein.

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Detection of a protein which binds specifically to the upstream region of the pcbAB gene in Penicillium chrysogenum

Chu

Renno²,

Saunders

1995

Curr Genet

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show abstract

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β-Laetam Antibiotics Produced by Genetically Engineered Filamentous Fungs

Cited by 1 publication

References 1 publication

Detection of a protein which binds specifically to the upstream region of the pcbAB gene in Penicillium chrysogenum

Detection of a protein which binds specifically to the upstream region of the pcbAB gene in Penicillium chrysogenum

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