β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to α-phase poly(vinylidene fluoride) films

Low, Yuen Kei Adarina; Zou, Xi; Fang, Yuming; Wang, Junling; Wang, Lin; Boey, Freddy Yin Chiang; Ng, Kee Woei

doi:10.1016/j.msec.2013.09.029

Cited by 22 publications

(12 citation statements)

References 28 publications

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“…Previous studies of osteoblast-PVDF interactions have demonstrated that these interactions can be exploited clinically to promote tissue growth 13 27 . The different phase types of PVDF films can affect the adhesion and proliferation of cells in different ways 28 . Surface topography and surface wettability also greatly influence cell adhesion and proliferation.…”

Section: Discussionmentioning

confidence: 99%

“…Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) was performed for the PPTi and NPTi using a Bruker spectrometer (Vector-22, Switzerland, Bruker Company), from 600 to 1500 cm −1 at a resolution of 1 cm −1 , to evaluate short-range molecular arrangements. The fraction of the β-phase within the crystalline region was calculated using the method first described by Osaki and Ishida 28 29 30 .…”

Section: Methods Sectionmentioning

confidence: 99%

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Polarization of an electroactive functional film on titanium for inducing osteogenic differentiation

Zhou

et al. 2016

Sci Rep

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To enhance the surface bioactivity of titanium (Ti) prostheses, an electroactive polyvinylidene fluoride (PVDF) film was prepared on a Ti substrate to provide a mimetic of the electrical microenvironment, which facilitated the performance of cell functions. The results of cell proliferation and differentiation assays indicated that polarization of the PVDF-Ti (PTi) altered its surface charge, thus inducing adhesion, proliferation and osteogenic differentiation of cells. The polarized PVDF-Ti (PPTi) may therefore find applications in bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Methods Sectionmentioning

confidence: 99%

Polarization of an electroactive functional film on titanium for inducing osteogenic differentiation

Zhou

et al. 2016

Sci Rep

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“…The piezoelectric constant of porous samples was measured in our previous studies. From previous works reported from our group it is possible to electrical pole the materials and measure the piezoelectric response (d 33 ) of porous samples [40].…”

Section: Polymer Phase Contentmentioning

confidence: 99%

Strategies for the development of three dimensional scaffolds from piezoelectric poly(vinylidene fluoride)

et al. 2016

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Cell supports based on electroactive materials, that generate electrical signal variations as a response to mechanical deformations and vice-versa, are gaining increasing attention for tissue engineering applications. In particular, poly(vinylidene fluoride), PVDF, has been proven to be suitable for these applications in the form of films and two-dimensional membranes. In this work, several strategies have been implemented in order to develop PVDF three-dimensional scaffolds. Three processing methods, including solvent casting with particulate leaching and three-dimensional nylon, and freeze extraction with poly(vinyl alcohol) templates are presented in order to obtain three-dimensional scaffolds with different architectures and interconnected porosity. Further, it is shown that the scaffolds are in the electroactive β-phase and show a crystallinity degree of ~ 45%. Finally, quasi-static mechanical measurements showed that an increase of the porous size within the scaffold leads to a tensile strengths and the Young's modulus decrease, allowing tuning scaffold properties for specific tissues.

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“…When these materials are implanted, these surface charges provide electrical cues locally to the damage sites, promote protein adhesion, modify membrane potential, regulate the voltage-gated calcium channels, and subsequently modulate cell behaviours [ 14 ]. They are shown to effectively control cell adhesion to the substrate, enhance cell proliferation, and trigger osteoinduction and bone regeneration in various studies [ 14 , 15 , 16 , 17 ]. Polyvinylidene difluoride (PVDF) is a promising candidate owing to its high piezoelectricity, thermal stability, and biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of PVDF-Coated PCL-TCP Scaffolds and Pulsed Electromagnetic Field on Osteogenesis

Dong

Suryani

Zhou

et al. 2021

IJMS

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Bone exhibits piezoelectric properties. Thus, electrical stimulations such as pulsed electromagnetic fields (PEMFs) and stimuli-responsive piezoelectric properties of scaffolds have been investigated separately to evaluate their efficacy in supporting osteogenesis. However, current understanding of cells responding under the combined influence of PEMF and piezoelectric properties in scaffolds is still lacking. Therefore, in this study, we fabricated piezoelectric scaffolds by functionalization of polycaprolactone-tricalcium phosphate (PCL-TCP) films with a polyvinylidene fluoride (PVDF) coating that is self-polarized by a modified breath-figure technique. The osteoinductive properties of these PVDF-coated PCL-TCP films on MC3T3-E1 cells were studied under the stimulation of PEMF. Piezoelectric and ferroelectric characterization demonstrated that scaffolds with piezoelectric coefficient d33 = −1.2 pC/N were obtained at a powder dissolution temperature of 100 °C and coating relative humidity (RH) of 56%. DNA quantification showed that cell proliferation was significantly enhanced by PEMF as low as 0.6 mT and 50 Hz. Hydroxyapatite staining showed that cell mineralization was significantly enhanced by incorporation of PVDF coating. Gene expression study showed that the combination of PEMF and PVDF coating promoted late osteogenic gene expression marker most significantly. Collectively, our results suggest that the synergistic effects of PEMF and piezoelectric scaffolds on osteogenesis provide a promising alternative strategy for electrically augmented osteoinduction. The piezoelectric response of PVDF by PEMF, which could provide mechanical strain, is particularly interesting as it could deliver local mechanical stimulation to osteogenic cells using PEMF.

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β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to α-phase poly(vinylidene fluoride) films

Cited by 22 publications

References 28 publications

Polarization of an electroactive functional film on titanium for inducing osteogenic differentiation

Polarization of an electroactive functional film on titanium for inducing osteogenic differentiation

Strategies for the development of three dimensional scaffolds from piezoelectric poly(vinylidene fluoride)

Synergistic Effect of PVDF-Coated PCL-TCP Scaffolds and Pulsed Electromagnetic Field on Osteogenesis

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