β-Sitosterol Inhibits Rheumatoid Synovial Angiogenesis Through Suppressing VEGF Signaling Pathway

Qian, Kai; Zheng, Xue-Xia; Wang, Chen; Huang, Wen-Guang; Liu, Xiaobao; Xu, Shu-Di; Liu, Dan-Kai; Lin, Changsong

doi:10.3389/fphar.2021.816477

Cited by 29 publications

(17 citation statements)

References 42 publications

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“…We obtained the ingredients and target information from database and constructed the ingredient‐ target network, with the major active components being beta‐sitosterol, stigmasterol, kaempferol, luteolin, and formononetin, the former two of which belong to the family of phytosterols, similar to cholesterol in structure and present in plant cell membranes. Many studies have reported that they offer anti‐inflammatory, antidiabetic and antioxidative benefits (Bao et al., 2022; Pei et al., 2022; Qian et al., 2021).…”

Section: Discussionmentioning

confidence: 99%

Uncovering the mechanism of Naoxintong capsule against hypertension based on network analysis and in vitro experiments

Chen,

Hu,

Wang

et al. 2024

Chem Biol Drug Des

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Naoxintong capsule (NXT) is a clinical drug for the treatment of cardiovascular diseases, but its pharmacological mechanism against hypertension remains unclear. Data concerning the compounds and targets of NXT were obtained from the TCMSP and DrugBank, whereas data concerning hypertension‐related genes were obtained from DisGeNET. The network was analyzed and established by STRING and Cytoscape, and function enrichment was analyzed by GO and KEGG analysis. Molecular docking was performed to analyze the interaction between ingredients and targets, cellular activity was evaluated by MTT assay, and RT‐qPCR and western blot were used to evaluate the expressions of related genes. The results showed that 146 active therapeutic components can target hypertension‐related genes, and we found that core genes were mainly involved in the metabolism of lipids, lipopolysaccharides, the inflammatory signaling pathway, and the oxidative stress pathway. In addition, there was high affinity between the components of NXT and targets of hypertension, where the former can increase cell viability and reduce the expressions of NOX4, MCP‐1, BAX, TNF‐α and IL‐1β. Moreover, NXT inhibited the expressions of IL‐6 and Fis1, as well as increased the expression of MCL‐1. These results revealed the active compounds, hypertension targets, signaling pathways, and molecular mechanisms of NXT for treating hypertension, offering references for the clinical application of NXT and the treatment of hypertension.

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Section: Discussionmentioning

confidence: 99%

Uncovering the mechanism of Naoxintong capsule against hypertension based on network analysis and in vitro experiments

Chen,

Hu,

Wang

et al. 2024

Chem Biol Drug Des

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“…Qian et al's research showed that β -sitosterol could inhibit synovial angiogenesis by affecting the proliferation and migration of endothelial cells, thus reducing joint swelling and cartilage damage in CIA mice. It can inhibit the expression of the VEGF signaling pathway, slow joint inflammation, and improve the damage of bone and cartilage [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

A Network Pharmacology-Based Study of Potential Targets of Angelicae Pubescentis-Herba Taxilli Compound for the Treatment of Osteoarthritis

Zhao

2022

Computational and Mathematical Methods in Medicine

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Objective. Using network pharmacology and molecular docking, we explored the mechanism of Angelicae Pubescentis- (AP-) Herba Taxilli (HT) in the treatment of osteoarthritis (OA). Methods. We selected Traditional Chinese Medicine Systems Pharmacology platform (TCMSP) to filtrate the practical components and targets of AP-HT. The disease targets of “osteoarthritis (OA)” were collected by GeneCards, DrugBank, TTD, OMIM, and PharmGKB databases, and the component-target interaction network was established by Cytoscape 3.9.1. Then, we set the protein-protein interaction (PPI) network by the STRING platform and visualized by Cytoscape 3.9.1. We also conducted Gene Ontology (GO) analysis and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment analysis via the bioinformatics platform. Finally, we performed molecular docking using PyMOL 2.3.0 and AutoDock Vina software. Results. 11 potential compounds were selected, and 1007 OA disease targets were collected. Ninety-four main targets of the AP-HT compound in the treatment of OA have been defined. PPI network demonstrated that JUN, RELA, TNF, IL6, MAPK1, TP53, AKT1, FOS, IL10, and MYC might serve as the critical targets of AP-HT for the treatment of OA. Moreover, membrane raft, membrane microdomain, cellular response to chemical stress, and cytokine receptor binding may play essential roles in the treatment of OA via GO analysis. The main functional pathways involved in these critical targets include fluid shear stress and atherosclerosis, lipid and atherosclerosis, IL-17 signaling pathway, age-range signaling pathway in diabetic composites, and TNF signaling pathway via KEGG analysis. The results of molecular docking showed that the critical ingredients of AP-HT had an excellent affinity to related nuclear genes.

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“…β-Sitosterolis has already reported the improvement in RA by VEGF pathway. [40] By lowering MMP-8 secretion and synthesis, Stigmasterol therapy is said to ameliorate joint-related pathology to levels close to normal. This drop in the levels of metalloproteinase MMP-8 and inflammatory mediators is most likely brought on by a decrease in the activity of the transcription factors MAPKs and NF-kBp65.…”

Section: Discussionmentioning

confidence: 99%

Pharmacological Evaluation of Different Extracts of Asparagus officinalis (Asparagaceae) as an Analgesic, Anti-Inflammatory and Anti-arthritic Agent in Rats

Kumar¹,

Srivastava²,

Gupta³

et al. 2022

Pharmacogn. Res.

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Background: To treat the joint pain arial part of Asparagus officinalis (asparagus) has historically been used. However, its efficacy for rheumatoid arthritis has not been pharmaceutically evaluated. We explore the phytochemical analysis anti-inflammatory, analgesic and anti-arthritic activity of petroleum ether, ethanol and aqueous extracts of Asparagus officinalis aerial part. Materials and Methods: Tail-flick method was used to evaluate the analgesic activity anti-inflammatory activity was carried out using paw oedema induced with carrageenan and CFA induced arthritic model was used to evaluate the potential in anti-arthritic activity in rats. The Petroleum ether, ethanolic and aqueous extracts were dosed orally in three divided doses (75, 150 and 300 mg/ kg). For anti-inflammatory and analgesic activity diclofenac sodium at 10 mg/kg was used as standard, whereas in anti-arthritic model prednisolone at 5 mg/kg and methotrexate at 0.5 mg/ kg were used as standard. One-way ANOVA followed by Dunnett's multiple range test were used to analyse statistical significance between means. Results: The results revealed a dosecontrolled anti-inflammatory, anti-arthritic effect with different extracts whereas at some extent analgesic activity was observed. Four compounds were present and confirmed by LCMS/MS. The CFA model's findings showed improved defence against arthritic lesions and changes in body weight. Additionally, Asparagus officinalis significantly improved rheumatoid factor, changed WBCs, and favourably altered radiographic and histological alterations. Conclusion: The findings indicate that Asparagus officinalis is a strong anti-arthritic and anti-inflammatory compound that may be suggested for the treatment of both chronic and acute inflammation.

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β-Sitosterol Inhibits Rheumatoid Synovial Angiogenesis Through Suppressing VEGF Signaling Pathway

Cited by 29 publications

References 42 publications

Uncovering the mechanism of Naoxintong capsule against hypertension based on network analysis and in vitro experiments

Uncovering the mechanism of Naoxintong capsule against hypertension based on network analysis and in vitro experiments

A Network Pharmacology-Based Study of Potential Targets of Angelicae Pubescentis-Herba Taxilli Compound for the Treatment of Osteoarthritis

Pharmacological Evaluation of Different Extracts of Asparagus officinalis (Asparagaceae) as an Analgesic, Anti-Inflammatory and Anti-arthritic Agent in Rats

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