β-Tricalcium-phosphate stimulates the differentiation of dental follicle cells

Viale-Bouroncle, Sandra; Bey, Brigitte; Reichert, Torsten E.; Schmalz, Gottfried; Morsczeck, Christian

doi:10.1007/s10856-011-4345-0

Cited by 12 publications

(10 citation statements)

References 22 publications

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“…As we have learned from previous studies mechanical properties such as surface stiffness are decisive for a successful osteogenic differentiation of dental stem cells [13,14]. Moreover, we showed that bone substitute materials such as β-tricalcium phosphate (TCP) supports the osteogenic differentiation [10]. Our study proposed therefore that bone-like materials such as commercially available bone substitutes are superior for dental tissue engineering.…”

Section: Discussionmentioning

confidence: 70%

“…We showed that DFCs had a typical flattened-shaped morphology with close contacts to the bone substitute material [10]. Interestingly, the gene expression of osteogenic markers such as osteopontin or RUNX2 was increased, and the alkaline phosphatase (ALP) activity was induced on TCP in differentiated DFCs [10]. All these data support the assumption that TCP could be the optimal scaffold for a successful differentiation protocol of DFC.…”

Section: Introductionmentioning

confidence: 81%

“…Conversely, an optimal bone substitute material has not been identified so far for different dental stem cell types. In a recent study, we investigated, therefore, cell survival/proliferation and cell differentiation of DFCs in combination with a commercially available TCP [10]. Here, DFCs attached on TCP and cell numbers increased after 6 days of cultivation.…”

Section: Introductionmentioning

confidence: 99%

“…Here, DFCs attached on TCP and cell numbers increased after 6 days of cultivation. We showed that DFCs had a typical flattened-shaped morphology with close contacts to the bone substitute material [10]. Interestingly, the gene expression of osteogenic markers such as osteopontin or RUNX2 was increased, and the alkaline phosphatase (ALP) activity was induced on TCP in differentiated DFCs [10].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions

et al. 2015

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BackgroundDental stem cells in combination with implant materials may become an alternative to autologous bone transplants. For tissue engineering different types of soft and rigid implant materials are available, but little is known about the viability and the osteogenic differentiation of dental stem cells on these different types of materials. According to previous studies we proposed that rigid bone substitute materials are superior to soft materials for dental tissue engineering.MethodsWe evaluated the proliferation, the induction of apoptosis and the osteogenic differentiation of dental stem/progenitor cells on a synthetic bone-like material and on an allograft product. The soft materials silicone and polyacrylamide (PA) were used for comparison. Precursor cells from the dental follicle (DFCs) and progenitor cells from the dental apical papilla of retained third molar tooth (dNC-PCs) were applied as dental stem cells in our study.ResultsBoth dental cell types attached and grew on rigid bone substitute materials, but they did not grow on soft materials. Moreover, rigid bone substitute materials only sustained the osteogenic differentiation of dental stem cells, although the allograft product induced apoptosis in both dental cell types. Remarkably, PA, silicone and the synthetic bone substitute material did not induce the apoptosis in dental cells.ConclusionsOur work supports the hypothesis that bone substitute materials are suitable for dental stem cell tissue engineering. Furthermore, we also suggest that the induction of apoptosis by bone substitute materials may not impair the proliferation and the differentiation of dental stem cells.Electronic supplementary materialThe online version of this article (doi:10.1186/s40729-014-0002-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions

et al. 2015

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“…Although newly formed hard tissues were observed in all implants, a dentin-like hard tissue was observed when porous BCP was used [577]. Besides, incorporation of nano-sized HA into electrospun poly(ε-caprolactone)/gelatin scaffolds was found to enhance dental pulp stem cells differentiation towards an odontoblast-like phenotype both in vitro and in vivo [578]. The osteoblast marker bone sialoprotein was highly expressed on β-TCP scaffolds seeded by dental follicle cells but almost absent in differentiated dental follicle cells without β-TCP.…”

Section: Tissue Engineering Approachesmentioning

confidence: 92%

Calcium Orthophosphates (CaPo ) and Dentistry

Dorozhkin¹

2016

Bioceram Dev Appl

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Proteomics Approaches in the Identification of Molecular Signatures of Mesenchymal Stem Cells

Xiao

Chen

2012

Mesenchymal Stem Cells - Basics and Clinical Application I

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Mesenchymal stem cells (MSCs) are undifferentiated, multi-potent stem cells with the ability to renew. They can differentiate into many types of terminal cells, such as osteoblasts, chondrocytes, adipocytes, myocytes, and neurons. These cells have been applied in tissue engineering as the main cell type to regenerate new tissues. However, a number of issues remain concerning the use of MSCs, such as cell surface markers, the determining factors responsible for their differentiation to terminal cells, and the mechanisms whereby growth factors stimulate MSCs. In this chapter, we will discuss how proteomic techniques have contributed to our current knowledge and how they can be used to address issues currently facing MSC research. The application of proteomics has led to the identification of a special pattern of cell surface protein expression of MSCs. The technique has also contributed to the study of a regulatory network of MSC differentiation to terminal differentiated cells, including osteocytes, chondrocytes, adipocytes, neurons, cardiomyocytes, hepatocytes, and pancreatic islet cells. It has also helped elucidate mechanisms for growth factor-stimulated differentiation of MSCs. Proteomics can, however, not reveal the accurate role of a special pathway and must therefore be combined with other approaches for this purpose. A new generation of proteomic techniques have recently been developed, which will enable a more comprehensive study of MSCs.

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β-Tricalcium-phosphate stimulates the differentiation of dental follicle cells

Cited by 12 publications

References 22 publications

Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions

Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions

Calcium Orthophosphates (CaPo ) and Dentistry

Proteomics Approaches in the Identification of Molecular Signatures of Mesenchymal Stem Cells

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