γ-Aminobutyric acid action in guinea-pig ileal myenteric plexus

Kaplita, Paul V.; Waters, Donald H.; Triggle, David J.

doi:10.1016/0014-2999(82)90573-8

Cited by 109 publications

(40 citation statements)

References 19 publications

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“…In (b) of submaximal cholinergic contractions to trans-quantitatively in agreement with those found for the guinea-pig intestine, in which electrically-stimulated cholinergic nerves are moderately sensitive to GABA action through GABAB-receptors (Kaplita et al, 1982;Kleinrok & Kilbinger, 1983;Ong & Kerr, 1983).…”

Section: Gaba (>M)supporting

confidence: 82%

“…It is possible that a variable proportion of NANC inhibitory neurones represents a population of cells with low GABAB-receptor density and is therefore resistant to the effect of GABA. Our findings extend to the rabbit colon the observation that GABAB-receptors are associated with inhibition of resting and, to a greater extent, electrically-stimulated autonomic nerve activity both at the intestinal and extra-intestinal level (Bowery et al, 1981;Kaplita et al, 1982;Kleinrok & Kilbinger, 1983;Giotti et al, 1983;Ong & Kerr, 1983;Ronai et al, 1987). As far as peristalsis is concerned, GABA and baclofen inhibited the velocity of propulsion, while 3-APS was ineffective.…”

Section: Gaba (>M)supporting

confidence: 72%

“…GABAA-receptor activation in the rabbit colon is 3 lo 30 similar to that observed in the colon of guinea-pig (>M) (Krantis et al, 1980;Onori et al, 1984) or in rat duodenum (Maggi et al, 1984;Krantis & Harding, 1987) but differs from that in the guinea-pig ileum (Krantis et al, 1980;Kaplita et al, 1982;Krantis & Kerr, 1981b;Giotti et al, 1983;Ong & Kerr, 1987;Tonini et al, 1987) .…”

Section: Induction Ofpropulsive Activitymentioning

confidence: 54%

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An in vitro study of the relationship between GABA receptor function and propulsive motility in the distal colon of the rabbit

Tonini

Crema

Frigo

et al. 1989

British J Pharmacology

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1 The effects of y-aminobutyric acid (GABA), 3-aminopropane sulphonic acid (3-APS) and baclofen on spontaneous, electrically-induced and propulsive motility were investigated in rabbit distal colon. dependently decreased the velocity of propulsion of an intraluminally-distended balloon. This effect was antagonized by DAVA and GABA or baclofen desensitization and resistant to SR 95531 and picrotoxinin. These antagonists per se had no effect on propulsion. In preparations in which propulsion was slowed by hyoscine (1 yM), baclofen caused no consistent further depression of propulsive activity.5 Our results show that GABAA-and GABAB-receptors are present in rabbit colon. GABAA-receptor stimulation activates NANC inhibitory nerves without apparently affecting propulsion. GABAB-receptors are associated with a reduction of neural (mainly cholinergic) activity subserving muscular tone and peristalsis and appear to be located on both cholinergic and NANC inhibitory nerves. However, the persisting propulsive activity during suppression of GABAA-and GABAB-receptor function suggests that GABA in enteric neurones is not crucial for the neural circuitry subserving colonic peristalsis in this species.

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Section: Gaba (>M)supporting

confidence: 82%

Section: Gaba (>M)supporting

confidence: 72%

Section: Induction Ofpropulsive Activitymentioning

confidence: 54%

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An in vitro study of the relationship between GABA receptor function and propulsive motility in the distal colon of the rabbit

Tonini

Crema

Frigo

et al. 1989

British J Pharmacology

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“…In the enteric nervous system, an extensive distribution of GABAergic neurons and nerve fibres has been identified (Erd o & Wolff, 1990). GABA is known to stimulate cholinergic neurotransmission in the gut (Kaplita, Waters & Triggle, 1982). Recently, the involvement of GABA in the control of epithelial electrolyte transport has been demonstrated, which suggested a role for enteric GABAergic neurons in mucosal functions (MacNaughton, Pineau & Krantis, 1996).…”

Section: Discussionmentioning

confidence: 99%

Expression of functional GABA_A receptors in cholecystokinin‐secreting gut neuroendocrine murine STC‐1 cells

Glassmeier

Herzig

Höpfner

et al. 1998

The Journal of Physiology

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Gastrointestinal neuroendocrine (NE) cells synthesize, store and secrete γ‐aminobutyric acid (GABA). Recently, an autocrine‐paracrine function of GABA has been proposed for secretion from NE cells. To search for functional GABAA receptors in NE gut cells, we performed whole‐cell and perforated‐patch‐clamp studies in the intestinal cholecystokinin (CCK)‐secreting NE cell line STC‐1. Application of GABA evoked currents in STC‐1 cells. These effects were mimicked by muscimol, an agonist of GABAA receptors, and blocked by picrotoxin or bicuculline, antagonists of GABAA receptors. The GABA‐ or muscimol‐activated currents reversed near 0 mV, which under the recording conditions used was consistent with the activation of the GABAA receptor‐Cl− channel complex. In contrast to the effect on most neurons, GABA as well as muscimol led to a (reversible) depolarization of the membrane potential of STC‐1 cells. Membrane depolarization in turn activated voltage‐gated Ca2+ channels and increased intracellular Ca2+ concentrations in STC‐1 cells. In accordance with the observed membrane depolarization and activation of voltage‐gated Ca2+ channels, both GABA and muscimol stimulated Ca2+‐dependent CCK release. In contrast, bicuculline inhibited the GABA‐induced secretion of CCK. Using the reverse transcription‐polymerase chain reaction (RT‐PCR), mRNA of the GABAA receptor subunits α2, α3, α5, β1, β3 and δ could be detected in STC‐1 cells. In summary, we have shown that the CCK‐secreting gut NE cell line STC‐1 expresses functional GABAA receptors and that GABA stimulates CCK release. Thus, GABA is involved in the fine tuning of CCK secretion from the gut NE cell line STC‐1.

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“…These findings are in agreement with the observations in the guinea-pig and rabbit distal colon (Krantis et al, 1980;Giotti et al, 1985;Tonini et al, 1989) and in the rat duodenum (Maggi et al, 1984): GABA activates GABAA receptors resulting in NANC-induced relaxations. In contrast, stimulation of the GABAA receptors in the guinea-pig ileum (Krantis et al, 1980;Kaplita et al, 1982;Giotti et al, 1983) and in the cat colon (Taniyama et al, 1987) triggered cholinergic post-ganglionic neurones which resulted in a contractile response.…”

Section: Discussionmentioning

confidence: 94%

GABA_A receptor‐mediated stimulation of non‐adrenergic non‐cholinergic neurones in the dog ileocolonic junction

Boeckxstaens

Pelckmans

Rampart

et al. 1990

British J Pharmacology

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1 The inhibitory effects of y-aminobutyric acid (GABA), the GABAA receptor agonist homotaurine and the GABAB receptor agonist (± )-baclofen were investigated on circular muscle strips of the dog terminal ileum and ileocolonic junction. 2 In the presence of atropine, GABA and homotaurine induced concentration-dependent relaxations, similar to the non-adrenergic non-cholinergic (NANC-mediated relaxations evoked by electrical stimulation or by acetylcholine. The ileocolonic junction was more sensitive to GABA and homotaurine than the ileum. (+)-Baclofen had no effect. Cross desensitization only occurred between GABA and homotaurine. 3 The GABAA receptor antagonist bicuculline shifted the concentration-response curves to GABA and homotaurine to the right. The maximal relaxation to GABA remained unaffected. 4 GABA-induced relaxations were not inhibited by timolol, guanethidine, domperidone, hexamethonium and desensitization to ATP, but were abolished by tetrodotoxin. 5Bicuculline, and pretreatment with GABA or ( )-baclofen had no effect on the NANC-evoked relaxations to electrical stimulation and acetylcholine. 6 In conclusion, GABA stimulates GABAA receptors located on inhibitory NANC neurones in the dog ileocolonic junction. Our results suggest that it is unlikely that GABA is the final inhibitory NANC neurotransmitter.

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γ-Aminobutyric acid action in guinea-pig ileal myenteric plexus

Cited by 109 publications

References 19 publications

An in vitro study of the relationship between GABA receptor function and propulsive motility in the distal colon of the rabbit

An in vitro study of the relationship between GABA receptor function and propulsive motility in the distal colon of the rabbit

Expression of functional GABA_A receptors in cholecystokinin‐secreting gut neuroendocrine murine STC‐1 cells

GABA_A receptor‐mediated stimulation of non‐adrenergic non‐cholinergic neurones in the dog ileocolonic junction

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γ-Aminobutyric acid action in guinea-pig ileal myenteric plexus

Cited by 109 publications

References 19 publications

An in vitro study of the relationship between GABA receptor function and propulsive motility in the distal colon of the rabbit

An in vitro study of the relationship between GABA receptor function and propulsive motility in the distal colon of the rabbit

Expression of functional GABAA receptors in cholecystokinin‐secreting gut neuroendocrine murine STC‐1 cells

GABAA receptor‐mediated stimulation of non‐adrenergic non‐cholinergic neurones in the dog ileocolonic junction

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Expression of functional GABA_A receptors in cholecystokinin‐secreting gut neuroendocrine murine STC‐1 cells

GABA_A receptor‐mediated stimulation of non‐adrenergic non‐cholinergic neurones in the dog ileocolonic junction