γ-Irradiation sterilized bone strengthened and toughened by ribose pre-treatment

Willett, Thomas L.; Burton, Brianne; Woodside, Mitchell; Wang, Zhirui; Gaspar, Anne; Attia, Tarik

doi:10.1016/j.jmbbm.2015.01.003

Cited by 23 publications

(15 citation statements)

References 41 publications

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“…Our results are consistent with previous radiation studies, and provide novel insight into the effect of ex vivo ionizing radiation on bone mechanics. In accordance with previous investigations of cortical bone, we observed a reduction in both monotonic and fatigue properties at a dose equivalent to nominal allograft sterilization of ~30,000 ± 5,000 Gy [10,15,20,[36][37][38], and a reduction of monotonic strength at 17,000 Gy [11]. While our study is consistent with earlier observations, our findings expand upon previous knowledge in three important areas.…”

Section: Discussionsupporting

confidence: 93%

Effects of ex vivo ionizing radiation on collagen structure and whole-bone mechanical properties of mouse vertebrae

Pendleton

Emerzian

Liu

et al. 2019

Bone

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Bone can become brittle when exposed to ionizing radiation across a wide range of clinically relevant doses that span from radiotherapy (accumulative 50 Gy) to sterilization (~35,000 Gy). While irradiation-induced embrittlement has been attributed to changes in the collagen molecular structure, the relative role of collagen fragmentation versus non-enzymatic collagen crosslinking remains unclear. To better understand the effects of radiation on the bone material without cellular activity, we conducted an ex vivo x-ray radiation experiment on excised mouse lumbar vertebrae. Spinal tissue from twenty-week old, female, C57BL/6J mice were randomly assigned to a single x-ray radiation dose of either 0 (control), 50, 1,000, 17,000, or 35,000 Gy. Measurements were made for collagen fragmentation, non-enzymatic collagen crosslinking, and both monotonic and cyclic-loading compressive mechanical properties. We found that the group differences for mechanical properties were more consistent with those for collagen fragmentation than for non-enzymatic collagen crosslinking. Monotonic strength at 17,000 and 35,000 Gy was lower than that of the control by 50% and 73% respectively, (p < 0.001) but at 50 and 1,000 Gy was not different than the control. Consistent with those trends, collagen fragmentation only occurred at 17,000 and 35,000 Gy. By contrast, non-enzymatic collagen crosslinking was greater than control for all radiation doses (p < 0.001). All results were consistent both for monotonic and cyclic loading conditions. We conclude that the reductions in bone compressive monotonic strength and fatigue life due to ex vivo ionizing radiation are more likely caused by fragmentation of the collagen backbone than any increases in non-enzymatic collagen crosslinks.

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Section: Discussionsupporting

confidence: 93%

Effects of ex vivo ionizing radiation on collagen structure and whole-bone mechanical properties of mouse vertebrae

Pendleton

Emerzian

Liu

et al. 2019

Bone

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“…The harvested bone allografts then underwent one of three treatments: (1) no treatment; (2) conventional γ‐irradiation; and (3) ribose‐pretreatment followed by γ‐irradiation . For the ribose pre‐treated group, the harvested bone was incubated in 1.2 M ribose solution for 24 h at 60°C.…”

Section: Methodsmentioning

confidence: 99%

“…In this way, the collagen connectivity, which would otherwise be degraded following irradiation, can be reinforced and strengthened by the newly created crosslinks. Biomechanical testing of cortical bone pretreated ex vivo with our novel sterilization technique showed protection of the mechanical properties otherwise lost to conventional irradiation . Both human and bovine bone samples treated with ribose prior to irradiation showed protection of ultimate strength, strain‐to‐failure, and work‐to‐fracture.…”

mentioning

confidence: 87%

“…Biomechanical testing of cortical bone pretreated ex vivo with our novel sterilization technique showed protection of the mechanical properties otherwise lost to conventional irradiation. [29][30][31] Both human and bovine bone samples treated with ribose prior to irradiation showed protection of ultimate strength, strain-to-failure, and work-to-fracture.…”

mentioning

confidence: 99%

“…irradiation; and (3) ribose-pretreatment followed by g-irradiation. 31 For the ribose pre-treated group, the harvested bone was incubated in 1.2 M ribose solution for 24 h at 60˚C. Then, irradiation sterilization of the both irradiated and ribose pretreated allografts were conducted with the allografts packed on dry ice and irradiated at 33 kGy using a 60 Co source.…”

mentioning

confidence: 99%

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Pre‐clinical evaluation of bone allograft toughened with a novel sterilization method: An in vivo rabbit study

Park¹,

Zhang

Attia

et al. 2019

Journal Orthopaedic Research

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Bone allografts often undergo γ‐irradiation sterilization to decrease infection risk. However this consequently degrades bone collagen and makes the allograft brittle. Our laboratory has previously found that pre‐treatment with ribose ex vivo protects the bone. However, it remains unclear whether or not ribose‐treated γ‐irradiated allografts are able to unite and remodel in vivo. Using New Zealand White rabbits (NZWr), we aimed to evaluate if ribose‐treated allografts can unite with host bone (compared to untreated (fresh‐frozen) and conventionally‐irradiated allografts). A critically‐sized defect was created in the radii of NZWr and reconstructed with allografts fixed with an intramedullary Kirschner wire. Healing and union were assessed at 2, 6, and 12 weeks post operation, with radiographs, µCT, static and dynamic histomorphometry, backscatter electron microscopy, and torsion testing. Intramedullary fixation achieved stable reconstructions and bony union in all groups and no differences were found in the radiographic and biomechanical parameters tested. Interestingly, γ‐irradiated allografts had significantly less bone volume due to evident resorption of the grafts. In contrast, ribose pre‐treatment protected γ‐irradiated allografts from this bone loss, with results similar to the fresh frozen controls. In conclusion, ribose‐pretreated γ‐irradiated allografts were able to unite in vivo. In addition to achieving bony union with host bone, ribose pre‐treatment may protect against allograft resorption. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res

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Biomechanical properties enhancement of gamma radiation-sterilized cortical bone using antioxidants

El-Hansi¹,

Sallam

Talaat

et al. 2020

Radiat Environ Biophys

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γ-Irradiation sterilized bone strengthened and toughened by ribose pre-treatment

Cited by 23 publications

References 41 publications

Effects of ex vivo ionizing radiation on collagen structure and whole-bone mechanical properties of mouse vertebrae

Effects of ex vivo ionizing radiation on collagen structure and whole-bone mechanical properties of mouse vertebrae

Pre‐clinical evaluation of bone allograft toughened with a novel sterilization method: An in vivo rabbit study

Biomechanical properties enhancement of gamma radiation-sterilized cortical bone using antioxidants

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