δ-Aminolaevulinic acid-induced porphyrin synthesis and photodynamic inactivation of Escherichia coli B.

Szocs, K.; Gábor, F.; Csı́k, Gabriella; Fidy, Judit

doi:10.1016/s1011-1344(99)00062-7

Cited by 48 publications

(32 citation statements)

References 20 publications

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“…The general result from the present study, that visible-light inactivation of gram-negative bacteria is much less efficient than the inactivation of gram-positive bacteria, agrees with results obtained by several other workers in studies involving bacteria pretreated with ␦-ALA (17,18,25,23). These studies with ␦-ALA used a range of light sources including blue light, 630-nm laser light, and white light.…”

Section: Resultssupporting

confidence: 91%

Inactivation of Bacterial Pathogens following Exposure to Light from a 405-Nanometer Light-Emitting Diode Array

Maclean

MacGregor

Anderson

et al. 2009

Appl Environ Microbiol

350

318

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This study demonstrates the susceptibility of a variety of medically important bacteria to inactivation by 405-nm light from an array of light-emitting diodes (LEDs), without the application of exogenous photosensitizer molecules. Selected bacterial pathogens, all commonly associated with hospital-acquired infections, were exposed to the 405-nm LED array, and the results show that both gram-positive and gram-negative species were successfully inactivated, with the general trend showing gram-positive species to be more susceptible than gram-negative bacteria. Detailed investigation of the bactericidal effect of the blue-light treatment on Staphylococcus aureus suspensions, for a range of different population densities, demonstrated that 405-nm LED array illumination can cause complete inactivation at high population densities: inactivation levels corresponding to a 9-log 10 reduction were achieved. The results, which show the inactivation of a wide range of medically important bacteria including methicillin-resistant Staphylococcus aureus, demonstrate that, with further development, narrow-spectrum 405-nm visible-light illumination from an LED source has the potential to provide a novel decontamination method with a wide range of potential applications.

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Section: Resultssupporting

confidence: 91%

Inactivation of Bacterial Pathogens following Exposure to Light from a 405-Nanometer Light-Emitting Diode Array

Maclean

MacGregor

Anderson

et al. 2009

Appl Environ Microbiol

350

318

View full text Add to dashboard Cite

show abstract

“…Heme biosynthesis is regulated at the ALA synthesis step through feedback inhibition that is dependent on the heme concentration (42,44). In the presence of exogenous ALA, however, heme biosynthesis is not repressed, and therefore a large amount of heme precursor porphyrin(ogen)s is produced (32). It also has been reported that protoporphyrinogen synthase, which is encoded by hemF, is inhibited by protoporphyrin (6).…”

Section: Discussionmentioning

confidence: 99%

TolC-Dependent Exclusion of Porphyrins in Escherichia coli

Tatsumi

Wachi

2008

J Bacteriol

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We found that Escherichia coli tolC mutants showed increased sensitivity to 5-aminolevulinic acid (ALA), a precursor of porphyrins. The tolC mutant cells grown in the presence of ALA showed a reddish brown color under visible light and a strong red fluorescence under near-UV irradiation. Fluorescence spectrometry and high-performance liquid chromatography analysis showed that the tolC mutant cells grown in the presence of ALA accumulated a large amount of coproporphyrin(ogen) intracellularly. In contrast, the wild-type cells produced coproporphyrin extracellularly. The tolC mutant cells grown in the presence of ALA, which were capable of surviving in the dark, were killed by near-UV irradiation, suggesting that the intracellular coproporphyrin(ogen) renders these cells photosensitive. These results suggest that the TolC-dependent efflux system is involved in the exclusion of porphyrin(ogen)s in E. coli.

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“…Inactivation of Helicobacter pylori by PDT was reported to occur in vitro (11) and ex vivo (13). Using the endogenous PS ␦-aminolevulinic acid, van der Meulen et al (25) reported a 4-log reduction in viable cells of planktonic Haemophilus parainfluenzae, while Szocs et al (21) observed 2-log killing of Escherichia coli. In addition to endogenous photoactive compounds, exogenous PS, such as hematoporphyrins, have also been reported to be effective when they are used for PDT and directed against Bacillus subtilis and Streptococcus faecalis (19).…”

mentioning

confidence: 99%

Use of Merocyanine 540 for Photodynamic Inactivation of Staphylococcus aureus Planktonic and Biofilm Cells

Lin

Chen

Huang

2004

Appl Environ Microbiol

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δ-Aminolaevulinic acid-induced porphyrin synthesis and photodynamic inactivation of Escherichia coli B.

Cited by 48 publications

References 20 publications

Inactivation of Bacterial Pathogens following Exposure to Light from a 405-Nanometer Light-Emitting Diode Array

Inactivation of Bacterial Pathogens following Exposure to Light from a 405-Nanometer Light-Emitting Diode Array

TolC-Dependent Exclusion of Porphyrins in Escherichia coli

Use of Merocyanine 540 for Photodynamic Inactivation of Staphylococcus aureus Planktonic and Biofilm Cells

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