ε-Poly-l-lysine as an efficient cartilage penetrating and residing drug carrier with high intraarticular injection safety for treating osteoarthritis

Lin, Junqing; Hu, Wencheng; Gao, Tao; Bao, Bingbo; Li, Xingwei; Huang, Tengli; Sun, Yi; Shen, Junjie; Xu, Hannan; Zhu, Kenny Q.; Zhu, Hongyi; Yang, Yunlong; Zheng, Xianyou

doi:10.1016/j.cej.2021.133018

Cited by 12 publications

(15 citation statements)

References 48 publications

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“…Moreover, in the spectrum of SA, the asymmetric COO – stretching vibration peak of SA is at 1609 cm –1 , which is also observed in the FTIR spectrum of MCs, suggesting a successful adsorption of SA. − In addition, the zeta potential of HNTs changes from −25.2 to 30.9 mV after adsorption of ε-PLL and further changes to −30.2 mV after the next adsorption of SA (Figure d). These changes of zeta potential originated from protonated α-amino groups in positively charged ε-PLL molecules and −COO – groups in negatively charged SA. ,, In addition, the absolute value of zeta potential is over 30 mV, suggesting that the encapsulation system is stable. − Combining the FTIR results with the zeta potential results, it can be proved that the encapsulation of ε-PLL and SA on HNTs is successful. In addition, the fabricated MCs were also characterized by SEM and TEM techniques.…”

Section: Resultsmentioning

confidence: 96%

“…These changes of zeta potential originated from protonated α-amino groups in positively charged ε-PLL molecules and −COO − groups in negatively charged SA. 8,37,38 In addition, the absolute value of zeta potential is over 30 mV, suggesting that the encapsulation system is stable. 39−41 Combining the FTIR results with the zeta potential results, it can be proved that the encapsulation of ε-PLL and SA on HNTs is successful.…”

Section: Fabrication and Characterization Of Mcsmentioning

confidence: 99%

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Designing Smart Microcapsules with Natural Polyelectrolytes to Improve Self-Healing Performance for Water-Based Polyurethane Coatings

Njuko

Meng

et al. 2022

ACS Appl. Mater. Interfaces

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Active anticorrosive organic coatings adopting microcapsules (MCs) have lately attracted extensive attention as they were proven to be effective to minimize metal corrosions and offer a long-lasting protection performance. Herein, a novel environmental-friendly active corrosion protection system was designed for aluminum alloy 2024 (AA2024) based on water-based polyurethane coatings with the addition of water and alkaline pHresponsive smart MCs, which is fabricated by utilizing 2mercaptobenzothiazole (2-MBT) as an inhibitor, halloysite clay nanotubes (HNTs) as an inhibitor carrier, and the natural polyelectrolytes ε-poly-L-lysine (ε-PLL) and sodium alginate (SA) as layer-by-layer (LBL) encapsulation polyelectrolytes. Salt spray tests and electrochemical measurements prove that the scratched coatings with embedded MCs possess an excellent self-healing performance by forming an adsorption layer of released 2-MBT on the AA2024 surface, thereby providing over 90% inhibition efficiency within 6 days' immersion. The UV−vis spectrophotometer results further showed that the release of 2-MBT is a three-stage long-term process sensitive to water and alkaline pH value, while the outward release rate is both regulated by the solubility of 2-MBT and the SA layer. The fabricated MCs not only offer a great promise to provide an excellent self-healing performance but also shed light on the future design of advanced MCs on demand based on the LBL technique.

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Section: Resultsmentioning

confidence: 96%

Section: Fabrication and Characterization Of Mcsmentioning

confidence: 99%

Designing Smart Microcapsules with Natural Polyelectrolytes to Improve Self-Healing Performance for Water-Based Polyurethane Coatings

Njuko

Meng

et al. 2022

ACS Appl. Mater. Interfaces

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“…Th apoptosis level of chondrocytes was detected by using terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) kits in accordance with the manufacturer’s protocol as previously described ( 16 ). Specially, the pretreated cell smears were fixed with 4% paraformaldehyde for 30 minutes and incubated with 20 μg/ml proteinase K for 10 minutes.…”

Section: Methodsmentioning

confidence: 99%

Identification and comprehensive analysis of circRNA–miRNA–mRNA regulatory networks in osteoarthritis

et al. 2023

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Osteoarthritis (OA) is a common orthopedic degenerative disease, leading to high disability in activities of daily living. There remains an urgent need to identify the underlying mechanisms and identify new therapeutic targets in OA diagnosis and treatment. Circular RNAs (circRNAs) play a role in the development of multiple diseases. Many studies have reported that circRNAs regulate microRNAs (miRNAs) through an endogenous competitive mechanism. However, it remains unclear if an interplay between circRNAs, miRNAs, and target genes plays a deeper regulatory role in OA. Four datasets were downloaded from the GEO database, and differentially expressed circRNAs (DECs), differentially expressed miRNAs (DEMs), and differentially expressed genes (DEGs) were identified. Functional annotation and pathway enrichment analysis of DEGs and DECs were carried out to determine the main associated mechanism in OA. A protein–protein network (PPI) was constructed to analyze the function of, and to screen out, hub DEGs in OA. Based on the artificial intelligence prediction of protein crystal structures of two hub DEGs, TOP2A and PLK1, digitoxin and oxytetracycline were found to have the strongest affinity, respectively, with molecular docking. Subsequently, overlapping DEMs and miRNAs targeted by DECs obtained target DEMs (DETMs). Intersection of DEGs and genes targeted by DEMs obtained target DEGs (DETGs). Thus, a circRNA–miRNA–mRNA regulatory network was constructed from 16 circRNAs, 32 miRNAs, and 97 mRNAs. Three hub DECs have the largest number of regulated miRNAs and were verified through in vitro experiments. In addition, the expression level of 16 DECs was validated by RT-PCR. In conclusion, we constructed a circRNA–miRNA–mRNA regulatory network in OA and three new hub DECs, hsa_circ_0027914, hsa_circ_0101125, and hsa_circ_0102564, were identified as novel biomarkers for OA.

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“…The effectiveness of OA model inducement was evaluated and analyzed using behavioural and histological measures. 13 , 14 The motivation for our study was to explore the advantages of microsurgical techniques in DMM murine models, to improve the stability of modelling, and decrease experimental cost, laying a strong foundation for follow-up OA-related basic research.…”

Section: Introductionmentioning

confidence: 99%

Modelling osteoarthritis in mice via surgical destabilization of the medial meniscus with or without a stereomicroscope

Lin

Wei

et al. 2022

Bone & Joint Research

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Aims To evaluate inducing osteoarthritis (OA) by surgical destabilization of the medial meniscus (DMM) in mice with and without a stereomicroscope. Methods Based on sample size calculation, 70 male C57BL/6 mice were randomly assigned to three surgery groups: DMM aided by a stereomicroscope; DMM by naked eye; or sham surgery. The group information was blinded to researchers. Mice underwent static weightbearing, von Frey test, and gait analysis at two-week intervals from eight to 16 weeks after surgery. Histological grade of OA was determined with the Osteoarthritis Research Society International (OARSI) scoring system. Results Surgical DMM with or without stereomicroscope led to decrease in the mean of weightbearing percentages (-20.64% vs -21.44%, p = 0.792) and paw withdrawal response thresholds (-21.35% vs -24.65%, p = 0.327) of the hind limbs. However, the coefficient of variation (CV) of weight-bearing percentages and paw withdrawal response thresholds in naked-eye group were significantly greater than that in the microscope group (19.82% vs 6.94%, p < 0.001; 21.85% vs 9.86%, p < 0.001). The gait analysis showed a similar pattern. Cartilage degeneration was observed in both DMM-surgery groups, evidenced by increased OARSI scores (summed score: 11.23 vs 11.43, p = 0.842), but the microscope group showed less variation in OARSI score than the naked-eye group (CV: 21.03% vs 32.44%; p = 0.032). Conclusion Although surgical DMM aided by stereomicroscope is technically difficult, it produces a relatively more homogeneous OA model in terms of the discrete degree of pain behaviours and histopathological grading when compared with surgical DMM without stereomicroscope. Cite this article: Bone Joint Res 2022;11(8):518–527.

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ε-Poly-l-lysine as an efficient cartilage penetrating and residing drug carrier with high intraarticular injection safety for treating osteoarthritis

Cited by 12 publications

References 48 publications

Designing Smart Microcapsules with Natural Polyelectrolytes to Improve Self-Healing Performance for Water-Based Polyurethane Coatings

Designing Smart Microcapsules with Natural Polyelectrolytes to Improve Self-Healing Performance for Water-Based Polyurethane Coatings

Identification and comprehensive analysis of circRNA–miRNA–mRNA regulatory networks in osteoarthritis

Modelling osteoarthritis in mice via surgical destabilization of the medial meniscus with or without a stereomicroscope

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