ε-Poly-l-Lysine/plasmid DNA nanoplexes for efficient gene delivery in vivo

Mandal, Haimanti; Katiyar, Sameer S.; Swami, Rajan; Katare, Parmeshwar B.; Meka, Anand Kumar; Banerjee, Sanjay K.; Popat, Amirali; Kushwah, Varun

doi:10.1016/j.ijpharm.2018.03.021

Cited by 62 publications

(40 citation statements)

References 34 publications

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“…Besides, they can display different chemistries and architectures [93]. CPs include commercially sourced materials ( Figure 2), such as poly-L-lysines (PLLs) [94,95], poly(ethyleneimines) (PEIs) [96,97], poly(amidoamines) (PAMAMs) [64,[98][99][100][101], poly [2-(dimethyamino)ethyl methacrylates] (PDMAEMs) [102], and chitosans (CSs) [103][104][105][106], used as received or reacted and functionalized [63,[107][108][109][110], and those purposely synthesized [111]. Of note, the efficacy of a transfectant strongly depends on some chemical and geometrical features.…”

Section: Cationic Polymers (Cps)mentioning

confidence: 99%

Non-Viral in Vitro Gene Delivery: It is Now Time to Set the Bar!

et al. 2020

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Transfection by means of non-viral gene delivery vectors is the cornerstone of modern gene delivery. Despite the resources poured into the development of ever more effective transfectants, improvement is still slow and limited. Of note, the performance of any gene delivery vector in vitro is strictly dependent on several experimental conditions specific to each laboratory. The lack of standard tests has thus largely contributed to the flood of inconsistent data underpinning the reproducibility crisis. A way researchers seek to address this issue is by gauging the effectiveness of newly synthesized gene delivery vectors with respect to benchmarks of seemingly well-known behavior. However, the performance of such reference molecules is also affected by the testing conditions. This survey points to non-standardized transfection settings and limited information on variables deemed relevant in this context as the major cause of such misalignments. This review provides a catalog of conditions optimized for the gold standard and internal reference, 25 kDa polyethyleneimine, that can be profitably replicated across studies for the sake of comparison. Overall, we wish to pave the way for the implementation of standardized protocols in order to make the evaluation of the effectiveness of transfectants as unbiased as possible.

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Section: Cationic Polymers (Cps)mentioning

confidence: 99%

Non-Viral in Vitro Gene Delivery: It is Now Time to Set the Bar!

et al. 2020

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“…Upon interacting with nucleic acids, PLL condensed into nanoassemblies with tunable charges and molecular masses varying from a few thousand to nearly one million Daltons [32]. An in vitro and in vivo comparison of PLL/pDNA peptiplexes and SuperFect ® polyplexes suggested that peptiplexes have improved transfection efficacy and are a safer non-viral delivery system [131]. In another study, incorporation of star-shaped PLL/pDNA peptiplexes into a collagen scaffold, resulted in successful in vivo transfection of autologous host cells, indicating the potential of this system in tissue engineering (Figure 9) [132].…”

Section: Peptidic Nanoassemblies With Cell-penetrating Propertiesmentioning

confidence: 99%

Peptide-Based Nanoassemblies in Gene Therapy and Diagnosis: Paving the Way for Clinical Application

et al. 2020

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Nanotechnology approaches play an important role in developing novel and efficient carriers for biomedical applications. Peptides are particularly appealing to generate such nanocarriers because they can be rationally designed to serve as building blocks for self-assembling nanoscale structures with great potential as therapeutic or diagnostic delivery vehicles. In this review, we describe peptide-based nanoassemblies and highlight features that make them particularly attractive for the delivery of nucleic acids to host cells or improve the specificity and sensitivity of probes in diagnostic imaging. We outline the current state in the design of peptides and peptide-conjugates and the paradigms of their self-assembly into well-defined nanostructures, as well as the co-assembly of nucleic acids to form less structured nanoparticles. Various recent examples of engineered peptides and peptide-conjugates promoting self-assembly and providing the structures with wanted functionalities are presented. The advantages of peptides are not only their biocompatibility and biodegradability, but the possibility of sheer limitless combinations and modifications of amino acid residues to induce the assembly of modular, multiplexed delivery systems. Moreover, functions that nature encoded in peptides, such as their ability to target molecular recognition sites, can be emulated repeatedly in nanoassemblies. Finally, we present recent examples where self-assembled peptide-based assemblies with “smart” activity are used in vivo. Gene delivery and diagnostic imaging in mouse tumor models exemplify the great potential of peptide nanoassemblies for future clinical applications.

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“…Apart from NDs, graphene oxide (GO), a Apart from cationic lipids, cationic polymers with different physicochemical properties are also often used as gene delivery systems, obtaining the corresponding polyplexes after genetic material is adsorbed on their surface or entrapped into the polymeric matrix. Most of those cationic polymers include chitosans [55], polyethylenimine [56], or poly (L-lysine) [57]. Moreover, hybrid compounds, made by a combination of both polycationic and polyanionic polymers, and with different organic and inorganic materials such as polymers, magnetite, or lipids can be used also to deliver genetic material for different purposes [58][59][60][61][62].…”

Section: Figure 2 A)mentioning

confidence: 99%

Niosome-Based Approach for In Situ Gene Delivery to Retina and Brain Cortex as Immune-Privileged Tissues

et al. 2020

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Non-viral vectors have emerged as a promising alternative to viral gene delivery systems due to their safer profile. Among non-viral vectors, recently, niosomes have shown favorable properties for gene delivery, including low toxicity, high stability, and easy production. The three main components of niosome formulations include a cationic lipid that is responsible for the electrostatic interactions with the negatively charged genetic material, a non-ionic surfactant that enhances the long-term stability of the niosome, and a helper component that can be added to improve its physicochemical properties and biological performance. This review is aimed at providing recent information about niosome-based non-viral vectors for gene delivery purposes. Specially, we will discuss the composition, preparation methods, physicochemical properties, and biological evaluation of niosomes and corresponding nioplexes that result from the addition of the genetic material onto their cationic surface. Next, we will focus on the in situ application of such niosomes to deliver the genetic material into immune-privileged tissues such as the brain cortex and the retina. Finally, as future perspectives, non-invasive administration routes and different targeting strategies will be discussed.

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ε-Poly-l-Lysine/plasmid DNA nanoplexes for efficient gene delivery in vivo

Cited by 62 publications

References 34 publications

Non-Viral in Vitro Gene Delivery: It is Now Time to Set the Bar!

Non-Viral in Vitro Gene Delivery: It is Now Time to Set the Bar!

Peptide-Based Nanoassemblies in Gene Therapy and Diagnosis: Paving the Way for Clinical Application

Niosome-Based Approach for In Situ Gene Delivery to Retina and Brain Cortex as Immune-Privileged Tissues

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