λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity

Wu, Wei; Zhou, Dongsheng; Xuan, Rongrong; Liu, Jingwangwei; Chen, Juanjuan; Han, Hui; Niu, Tingting; Li, Xingxing; Chen, Haimin; Wang, Feng

doi:10.1016/j.phrs.2021.105940

Cited by 11 publications

(6 citation statements)

References 42 publications

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“…In our study, IL-1β, TNF-α, IL-6, and IL-17A contents within the colon increased after CR infection, conforming to prior research regarding CR-induced colitis [40,64]. We also detected that colonic IL-17A contents were elevated after CR infection.…”

Section: Discussionsupporting

confidence: 91%

Ginkgo biloba Extract Preventively Intervenes in Citrobacter Rodentium-Induced Colitis in Mice

Chen

et al. 2023

Nutrients

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Inflammatory bowel disease (IBD) represents a highly recurrent gastrointestinal disorder and global public health issue. However, it lacks effective and safe strategies for its control. Although Ginkgo biloba extract (GBE) has been suggested to exhibit preventive and therapeutic activity for the control of IBD, whether its activity is associated with its ability to modulate intestinal microbiota remains to be addressed. To investigate the effect of GBE on controlling IBD, a Citrobacter Rodentium (CR)-induced mouse colitis model was used, and then histopathological examinations, biochemical assays, immunohistochemistry, and immunoblotting were performed to detect histological changes, cytokines, and tight junction (TJ) proteins in the intestine samples. We also studied 16s rRNA to detect changes in intestinal microbiota and used GC-MS to determine the microbiota-related metabolites short chain fatty acids (SCFAs). The results of our studies revealed that pre-treatment with GBE was sufficient for protecting the animals from CR-induced colitis. As a mechanism for GBE activity, GBE treatment was able to modulate the intestinal microbiota and increase the SCFAs capable of decreasing the pro-inflammatory factors and up-regulating the anti-inflammatory factors while elevating the intestinal-barrier-associated proteins to maintain the integrity of the intestines. Accordingly, our results led to a strong suggestion that GBE should be seriously considered in the preventive control of CR-induced colitis and in the development of effective and safe therapeutic strategies for controlling IBD.

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Section: Discussionsupporting

confidence: 91%

Ginkgo biloba Extract Preventively Intervenes in Citrobacter Rodentium-Induced Colitis in Mice

Chen

et al. 2023

Nutrients

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“…After extracting the total DNA, the bacterial 16S rDNA genes were amplified with sequencing primers, sample-specific barcodes, and 16S polymerase chain reaction (PCR) primers. 46 The amplified DNA was purified for the construction of sequencing libraries, and sequenced by an Illumina Novaseq 6000. Based on base calling analysis, raw data files gained from high-throughput sequencing were converted to raw sequenced sequences.…”

Section: ■ Introductionmentioning

confidence: 99%

Galactooligosaccharide or 2′-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice

Chen

Wang

Nie

et al. 2023

J. Agric. Food Chem.

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A high-fructose diet (HFrD) has been reported to exacerbate dextran sulfate sodium (DSS)-induced colitis. 2′-Fucosyllactose (FL) and galactooligosaccharide (GOS) have been shown, respectively, to have preventive and ameliorative effects on colitis, while limited research has explored whether GOS and FL may be equally protective or preventive in mice with HFrD. Here, we evaluated the protective effects of FL and GOS on colitis exacerbated by feeding HFrD and explored the underlying mechanisms. DSS-induced colitis was studied in four randomized C57BL/6J male mice (n = 8 mice/group). Among them, three groups were fed with HFrD, and two received either GOS or FL treatment, respectively. Gut microbial composition was analyzed by 16S rDNA gene sequencing. Intestinal barrier integrity and inflammatory pathway expression were measured using qPCR, immunofluorescence, and Western blot methods. Compared to the HFrD group, GOS or FL treatment increased the α-diversity of the gut microbiota, reduced the relative abundance of Akkermansia, and increased the content of short-chain fatty acids (SCFAs), respectively. Compared with the HFrD group, GOS or FL treatment improved the loss of goblet cells and the reduction of tight junction protein expression, thereby improving intestinal barrier integrity. Also, GOS or FL inhibited the LPS/TLR4/NF-κB signaling pathway and oxidative stress to suppress the inflammatory cascade compared with the HFrD group. These findings suggest that GOS or FL intake can alleviate HFrD-exacerbated colitis, with no significant difference observed between GOS and FL treatments.

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“…Colitis can also be induced by carboxymethyl cellulose and polysorbate 80, which are food additives known to promote immune cell infiltration and increase the levels of pro‐inflammatory cytokines in the colon (Wu et al., 2021).…”

Section: Chemically Induced Ibd Modelsmentioning

confidence: 99%

“…The severe colitis model can be obtained by combining chemical reagents with bacteria, or by using gene knockout mice as model and inducing by chemical reagents. For instance, oral administration of carrageenan can exacerbate inflammation and increase the clinical severity of intestinal tissue in mice with infectious colitis induced by Citrobacter rodentium (Wu et al., 2021).…”

Section: Other Ibd Modelmentioning

confidence: 99%

An evaluation of animal models for using bioactive compounds in the treatment of inflammatory bowel disease

Tie,

Chen,

Tan

2024

Food Frontiers

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Inflammatory bowel disease (IBD) is a prevalent chronic relapsing‐remitting disease that affects the digestive tract and reduces patients’ quality of life. Food bioactive compounds are a promising approach for nutritional intervention of IBD with minimal side effects. To evaluate their potential, researchers have developed various animal models that simulate human IBD, including chemically induced, spontaneous, genetically engineered, and adoptive transfer models. In this work, the main animal models, pathogenesis, symptoms, strengths, and limitations of IBD were discussed. Although these models could not perfectly replicate human IBD, they provided useful tools to study the disease's progression and therapeutic processes. In addition, taking these IBD animals as models, the specific experimental methods, intervention results, and mechanisms of food bioactive compounds to prevent or treat IBD were described in detail. The continuous improvement of animal models is crucial to gain a better understanding of IBD's etiology and pathogenesis and to evaluate the efficacy of food bioactive compounds for the effective control of IBD.

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λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity

Cited by 11 publications

References 42 publications

Ginkgo biloba Extract Preventively Intervenes in Citrobacter Rodentium-Induced Colitis in Mice

Ginkgo biloba Extract Preventively Intervenes in Citrobacter Rodentium-Induced Colitis in Mice

Galactooligosaccharide or 2′-Fucosyllactose Modulates Gut Microbiota and Inhibits LPS/TLR4/NF-κB Signaling Pathway to Prevent DSS-Induced Colitis Aggravated by a High-Fructose Diet in Mice

An evaluation of animal models for using bioactive compounds in the treatment of inflammatory bowel disease

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