μCB-seq: microfluidic cell barcoding and sequencing for high-resolution imaging and sequencing of single cells

Abstract

We present a platform for on-chip molecular barcoding that combines high-resolution imaging with genomic analysis, enabling multi-modal phenotypic measurements in single cells.

Paper-thin multilayer microfluidic devices with integrated valves

“…Combining SRS imaging with integrated valvemicrofluidics enables many new applications, including singlecell sorting based on high-resolution hyper-spectral SRS imaging. Joined with RNA sequencing, this can add information about chemical composition and morphological information to single cell profiles 32 for discovering new cell types and related marker genes.…”

μDamID: A Microfluidic Approach for Joint Imaging and Sequencing of Protein-DNA Interactions in Single Cells

“…The throughput of this platform can be increased to hundreds of cells per device by scaling up the design and incorporating features like multiplexed valve control ( Kim et al, 2017 ) and automated image processing and sorting. To scale this technology further, paired imaging and sequencing data could be obtained using spatially or optically registered DNA barcodes ( Cole et al, 2017 ; Nguyen et al, 2017 ; Yuan et al, 2018 ; Chen et al, 2020 ).…”

Microfluidic Compartmentalization Platforms for Single Cell Analysis

“…The integrated device allows the analysis of both spatial distribution and sequence identity, uncovering more variations of protein-DNA interactions. The group also presented another innovation, termed microfluidic cell barcoding and sequencing (μCB-seq), that pairs the imaging and sequencing information [ 41 ]. The compatibility of μCB-seq with standard microscopies makes it easy to be incorporated and implemented.…”