Abstract—Using a hard alloy (Co–WC) striker, ultrasonic impact treatment (UTT) of Ti–6Al–4V alloy samples obtained by electron-beam wire additive technology was carried out. Using X-ray diffraction analysis and transmission electron microscopy, it has been shown that UTT leads to the appearance of compressive macrostresses in the surface layers of the sample, elastic microdeformation in the crystal lattice of the alpha-phase, to the formation of a gradient structure from nanocrystalline at a depth of 5 μm to a submicrocrystalline structure of the alpha-phase at a depth from 15 to 40 microns.A nanocrystalline phase of titanium oxides is formed in the grains of the alpha phase. UTT leads to an increase in microhardness and fatigue life. A fractographic analysis of specimen fractures after cyclic tension in the low-cycle fatigue regime has been carried out.