The effect of the application of different norms of liquid pig manure on microflora composition and microbiological activity of leached chernozem, as well as on its sanitary and microbiological indicators, has been studied. It was found that at the maximum application rate, the number of bacteria increased to a greater extent than that of actinomycetes and fungi. At the same time, the genera Pseudomonas, Cellvibrio, and Bacillus predominate among the bacteria. Micromycetes of the genus Mukor and Aspergillus predominate among fungi. The nitrate content remained within acceptable limits, the mineralization coefficient for this variant is quite high. According to sanitary and microbiological indicators, when pig manure is applied in the maximum norm, the samples fall into the group of heavily polluted soil. According to the index of the coli-titer for enterobacteria, all the studied soil samples fall into the group of slightly polluted, the sanitary and epidemiological situation has not worsened. The application of the maximum manure rate had a positive effect on wheat yield.